Unlock instant, AI-driven research and patent intelligence for your innovation.

Collagenous fiber-based membrane material for rapidly detecting biogenic amine and preparation method thereof

A technology of collagen fibers and biogenic amines, which is applied in the direction of material analysis by observing the influence of chemical indicators, and analysis by making materials undergo chemical reactions, which can solve the problems of long time consumption, high equipment requirements, and high cost of detection reagents

Inactive Publication Date: 2020-10-27
DALIAN POLYTECHNIC UNIVERSITY
View PDF8 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, high performance liquid chromatography and capillary liquid chromatography-tandem mass spectrometry are mainly used to detect biogenic amines. Although these methods are accurate, they have the disadvantages of cumbersome operation, high equipment requirements, high cost of detection reagents, and long time consumption.
Samples with low biogenic amine content cannot be quickly qualitatively checked, which makes it time-consuming to detect biogenic amines in food. Therefore, it is necessary to establish a new method. This method quickly screens samples, so that qualified samples in the tested samples are quickly separated

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Collagenous fiber-based membrane material for rapidly detecting biogenic amine and preparation method thereof
  • Collagenous fiber-based membrane material for rapidly detecting biogenic amine and preparation method thereof
  • Collagenous fiber-based membrane material for rapidly detecting biogenic amine and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036]A collagen fiber-based membrane material for rapid detection of biogenic amines was prepared by tearing calfskin (3 years old): 2mol / LCa(OH) 2 Soak cowhide in aqueous solution for 12 hours, remove excess fat, rinse with tap water until pH = 7-8, then soak cowhide in hydrochloric acid solution with pH = 3 for 4 hours until the cortex is soft, then tear the swollen cowhide, Obtain collagen fibers. Dissolve the obtained collagen fibers with 1.25% solute content in acetic acid solution at pH=3, add 25% glycerol and 0.012% bromocresol green in dry matter, adjust pH=5 with 0.1mol / L NaOH solution After that, it was dried naturally for 48 hours to form a film.

[0037] Wet the membrane material with different concentrations of cadaverine (respectively 0mg / kg, 100mg / kg, 200mg / kg, 300mg / kg, 400mg / kg, 500mg / kg), and the color development results are as follows: figure 1 shown.

[0038] Depend on figure 1 It can be seen that the original picture before the membrane is wet is lig...

Embodiment 2

[0040] A commercially available calfskin (3-year-old) collagen fiber casing raw material was used to prepare a collagen fiber-based membrane material for rapid detection of biogenic amines: the purchased collagen fiber casing raw material (Devro Defule (Nantong) Technology Co., Ltd.) was mixed with 1% The solute content is dissolved in the acetic acid solution of pH=3, after adding the glycerin of 25% dry matter and 0.006% of bromocresol purple, without adjusting the pH, bake in a constant temperature and humidity box with a relative humidity of 50% at 25°C Dry for 24h to form a film.

[0041] Wet the membrane material with different concentrations of histamine (0mg / kg, 100mg / kg, 200mg / kg, 300mg / kg, 400mg / kg, 500mg / kg respectively), and the color development results are as follows: figure 2 shown.

[0042] Depend on figure 2 It can be seen that the original image before the membrane is wet is yellow, and the wet membrane cannot be unfolded well due to the low concentration...

Embodiment 3

[0044] A commercially available calfskin (3-year-old) collagen fiber casing raw material was used to prepare a collagen fiber-based membrane material for rapid detection of biogenic amines: the purchased collagen fiber raw material (Devro Defule (Nantong) Technology Co., Ltd.) was mixed with 2.5% The solute content is dissolved in the acetic acid solution of pH = 3, after adding glycerin with a dry matter amount of 25% and bromocresol green at 0.006%, without adjusting the pH, bake in a constant temperature and humidity box with a relative humidity of 50% at 25°C Dry for 24h to form a film.

[0045] Wet the membrane material with different concentrations of histamine (0mg / kg, 100mg / kg, 200mg / kg, 300mg / kg, 400mg / kg, 500mg / kg respectively), and the color development results are as follows: Figure 4 shown.

[0046] Depend on image 3 It can be seen that the original image of the membrane before wetting is pale yellow with high transparency and is relatively thin. Due to the lo...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a collagenous fiber-based membrane material for rapidly detecting biogenic amine and a preparation method thereof. According to the method, animal skin is used as a raw material, collagen fiber obtained by tearing after alkali washing, rinsing and acid swelling is used as a raw material, a plasticizer and a color developing agent are added, pH is adjusted or not adjusted, and the product can be used after drying and film forming. The detection membrane material has the characteristics of rapidness, convenience, low cost, sensitive detection and the like. The rapid detection method is a new means for rapidly and qualitatively detecting biogenic amine, and has far-reaching significance for popularizing food quality and safety.

Description

technical field [0001] The invention relates to the fields of animal product processing, food safety, and the like, and more specifically relates to a collagen fiber-based membrane material for rapid detection of biogenic amines and a preparation method thereof. Background technique [0002] Biogenic amine is a general term for a class of nitrogen-containing organic compounds with low molecular weight. According to the structure, biogenic amines are divided into aliphatic, aromatic and heterocyclic groups, which respectively include: putrescine, spermine, cadaverine, spermidine, etc.; phenylethylamine, tyramine, etc.; tryptamine, histamine, etc. The existence of biogenic amines is relatively common, and often exists in fermented foods, aquatic products, etc., such as: wine, sauces, etc. Some biogenic amines are beneficial to the human body, such as: histamine can regulate nerve activity and regulate blood pressure; tyramine and phenylethylamine have a pressor effect. Howev...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C08L89/00C08K5/053C08K5/46C08J5/18G01N21/78
CPCC08J5/18C08J2389/00C08K5/053C08K5/46G01N21/78
Inventor 牟光庆吴晓萌张俊鹏朱雪梅姜淑娟钱方妥彦峰
Owner DALIAN POLYTECHNIC UNIVERSITY