Method for extracting mixed tartaric acid and antibacterial essential oil from liquidambar formosana hance

A technology of Passepartout and essential oils, applied in food ingredients containing natural extracts, antibacterial drugs, pharmaceutical formulas, etc., can solve the problems of cumbersome extraction process, loss of fat-soluble beneficial ingredients, etc., and achieve simple extraction method and reduce waste Effect

Inactive Publication Date: 2020-10-30
贵州光正制药有限责任公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But it only extracts ursolic acid and oleanolic acid, but the fat-soluble beneficial ingredients in Ligustrum lucidum are lost, and the whole extraction process is relatively cumbersome

Method used

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  • Method for extracting mixed tartaric acid and antibacterial essential oil from liquidambar formosana hance
  • Method for extracting mixed tartaric acid and antibacterial essential oil from liquidambar formosana hance
  • Method for extracting mixed tartaric acid and antibacterial essential oil from liquidambar formosana hance

Examples

Experimental program
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Effect test

Embodiment 1

[0020] A method for extracting mixed fruit acids and antibacterial essential oils from Passepartout, comprising the following steps:

[0021] 1. Pre-treatment: dry Passepartout and roll it, and take the large particles after sieving, discard the powder and Passepartout stem impurities, soak the large particles in sodium hydroxide alkali solution for 1 hour, and then heat them at 60°C Soak in water for 2 hours, dry and crush to obtain Passepartout powder.

[0022] 2. Fermentation: inoculate the microbial agent in the culture medium and cultivate it for 3 days, and then irradiate the fermentation medium with a power of 400W for 4 seconds; mix the fermentation medium with Passepartout powder at a ratio of 1.5:1, and heat it at a temperature of 16°C. Fermentation for 7 days under the condition of humidity 65%;

[0023] Further, the microbial agent is prepared by mixing cellulase, pectinase and yeast in a ratio of 8:3:3.5.

[0024] 3. One-time extraction: The fermented mixture is...

Embodiment 2

[0029] A method for extracting mixed fruit acids and antibacterial essential oils from Passepartout, comprising the following steps:

[0030] 1. Pre-treatment: Dry Passepartout and roll it, and take large particles after sieving, discard the powder and Passepartout stem impurities, soak the large particles in sodium hydroxide alkali solution for 0.5h, and then use 50℃ Soak in hot water for 2 hours, dry and pulverize to obtain Passepartout powder.

[0031] 2. Fermentation: inoculate the microbial agent in the culture medium and cultivate it for 2 days, and then irradiate the fermentation medium with a power of 300W for 3 seconds; mix the fermentation medium with Passepartout powder at a ratio of 1:0.8, and heat it at a temperature of 12°C. Fermentation for 5 days under the condition of humidity 60%;

[0032] Further, the microbial agent is prepared by mixing cellulase, pectinase and yeast in a ratio of 6:2.5:2.

[0033] 3. One-time extraction: The fermented mixture is subject...

Embodiment 3

[0038] A method for extracting mixed fruit acids and antibacterial essential oils from Passepartout, comprising the following steps:

[0039] 1. Pre-treatment: dry Passepartout and then roll it, sieve and take the large particles, discard the powder and Passepartout stem impurities, soak the large particles in sodium hydroxide alkali solution for 1 hour, and then use hot water at 65°C Soak for 3 hours, dry and crush to obtain Passepartout powder.

[0040] 2. Fermentation: inoculate the microbial agent in the culture medium and cultivate it for 3 days, and then irradiate the fermentation medium with a power of 500W for 5 seconds; mix the fermentation medium with Passepartout powder at a ratio of 1.5:1.2, and heat it at a temperature of 18°C. Fermentation for 10 days under the condition of humidity 70%;

[0041] Further, the microbial agent is prepared by mixing cellulase, pectinase and yeast in a ratio of 10:4:4.5.

[0042]3. One-time extraction: The fermented mixture is subj...

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Abstract

The invention belongs to the technical field of traditional Chinese medicine extraction, and particularly relates to a method for extracting mixed tartaric acid and antibacterial essential oil from liquidambar formosana hance. The liquidambar formosana hance is extracted by adopting methods of pretreatment, primary extraction, gradient elution, secondary extraction and purification. Ursolic acid,oleanolic acid and volatile oil with an antibacterial effect can be extracted from the extract at the same time; the extraction method is simple, waste is reduced as much as possible, meanwhile, the diameters of inhibition zones of the extracted antibacterial essential oil on escherichia coli and bacillus subtilis reach 6.1 mm and 14.7 mm respectively, and the purities of ursolic acid and oleanolic acid reach 95.3% and 92.7% respectively.

Description

technical field [0001] The invention belongs to the technical field of traditional Chinese medicine extraction, and in particular relates to a method for extracting mixed fruit acids and antibacterial essential oils from Passepartout. Background technique [0002] Passepartout is the dry and mature infructescence of the Hamamelidaceae plant sweetgum. It is a traditional Chinese medicinal material. It has the functions of expelling wind, activating collaterals, diuresis, and dredging menstrual flow. It is used for arthralgia, numbness and spasm, swelling and swelling. , less milk, amenorrhea. [0003] Ursolic acid is a pentacyclic triterpenoid compound, which has various biological effects such as sedative, anti-inflammatory, antibacterial, anti-diabetic, and hypoglycemic, and also has obvious antioxidant function. Oleanolic acid is a pentacyclic triterpenoid isolated and extracted from the fruit of Gentianaceae Swerteria genus or Ligustrum lucidum. At present, it is relati...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/185C07J63/00A61K31/56A61P31/04A61K8/9789A61K8/63A61Q17/00A23L3/3508A23L3/3472
CPCA23L3/3472A23L3/3508A23V2002/00A61K8/63A61K31/56A61K36/185A61Q17/005A61K8/9789A61P31/04C07J63/008A23V2200/10A23V2250/21Y02A40/90Y02A50/30
Inventor 杨佳红熊伟王萍姚廷普陈兴菊陈云燕
Owner 贵州光正制药有限责任公司
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