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A kind of composite protein film and preparation method thereof

A technology of compounding protein and sericin, which is applied in the field of biomedical materials, can solve the problems of large damage to macromolecular structure and poor mechanical properties, and achieve the effects of rapid adhesion growth, improving strength and promoting regeneration.

Active Publication Date: 2021-12-24
SUZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] However, the regenerated silk (silk fibroin, sericin) protein material studied above has poor mechanical properties, and it is difficult to meet the requirements of specific tissues such as the cornea with high flexibility requirements. The key to the problem is the existing degumming method and extraction method. The method has great damage to the macromolecular structure of silk fibroin and sericin, and the degree of polymerization (molecular weight) of the macromolecule is an extremely important internal factor determining the performance of the macromolecule.

Method used

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Examples

Experimental program
Comparison scheme
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preparation example Construction

[0026] Aiming at the problems in the prior art that the silk fibroin macromolecules are severely damaged or the silk fibroin sericin composite solution is prepared separately, which affects the strength and flexibility of the silk fibroin sericin composite film, this application provides a composite protein film, which The composite protein film is prepared from a silk fibroin sericin composite solution. During the preparation of the composite protein film, a large molecular weight and high viscosity silk fibroin sericin composite solution is prepared by using deionized water temperature, degumming, and one-step dissolution method. This method can avoid The destruction of silk fiber macromolecules finally makes the silk fibroin in the prepared silk fibroin sericin composite solution have higher viscosity and large molecular weight, and introduces flexible chain segments, and then the composite protein film prepared by it has relatively high viscosity and high molecular weight. ...

Embodiment 1

[0041] 1. Take silkworm raw silk that has been boiled and reeled as raw material, take 1 kg of silkworm raw silk, take 50L of deionized preheated to 60°C according to the bath ratio of 1:50 (g / mL), put the raw silk into the raw silk at a constant temperature of 60°C and stir for treatment 1 to 10 minutes, then take it out and wash the silk with deionization at 25 to 30°C, dehydrate it, and dry it with hot air at 40 to 60°C;

[0042] 2. Put the dried silk in 90°C preheated deionized water according to the bath ratio of 1:50 (g / mL) and cook at a controlled constant temperature for 3 hours, then take out the silk containing sericin and wash it thoroughly with deionized water at 25-30°C , after dehydration, dry with hot air at 40-60°C;

[0043] 3. Weigh the dried silkworm silk and dissolve it in a 9.3M lithium bromide aqueous solution at a bath ratio of 1:25 (g / mL), and treat at 65°C until the silk is completely dissolved to obtain a silkworm silk fibroin sericin complex solution;...

Embodiment 2

[0048] 1. Take silkworm raw silk that has been boiled and reeled as raw material, take 1 kg of silkworm raw silk, take 50L of deionized preheated to 60°C according to the bath ratio of 1:50 (g / mL), put the raw silk into the raw silk at a constant temperature of 60°C and stir for treatment 1 to 10 minutes, then take it out and wash the silk with deionization at 25 to 30°C, dehydrate it, and dry it with hot air at 40 to 60°C;

[0049] 2. The dried silk is placed in 90°C preheated deionized water and boiled at a controlled temperature for 7 hours according to the bath ratio of 1:50 (g / mL), and the silk containing sericin is taken out and fully cleaned with deionized water at 25-30°C , after dehydration, dry with hot air at 40-60°C;

[0050] 3. Weigh the dried silkworm silk and dissolve it in a 9.3M lithium bromide aqueous solution at a bath ratio of 1:25 (g / mL), and treat at 65°C until the silk is completely dissolved to obtain a silkworm silk fibroin sericin complex solution;

...

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Abstract

The invention provides a method for preparing a composite protein film, comprising the following steps: A) placing silk in warm water for pretreatment, then placing it in water at 65-95°C for treatment, and drying to obtain initially treated silk; B) Treat the initially treated silk in a neutral salt solution, and then purify the obtained silk fibroin sericin compound solution to obtain a silk fibroin sericin compound solution; C) prepare the silk fibroin sericin compound solution Concentrating, reacting with additives, and drying to obtain a composite protein film; the additives are water-soluble flexible chain small molecules and biological cross-linking agents, or water-soluble flexible chain small molecule biological cross-linking agents. The present application adopts a mild degumming and one-step dissolution method to prepare a silk fibroin sericin compound solution, and uses the silk fibroin sericin compound solution to prepare a high-strength and flexible composite protein film.

Description

technical field [0001] The invention relates to the technical field of biomedical materials, in particular to a composite protein film and a preparation method thereof. Background technique [0002] In the 1990s, the concept of tissue engineering was proposed, and then the research on tissue engineering materials developed rapidly. At present, tissue engineering scaffold materials include synthetic polymer materials and natural polymer materials. Synthetic polymer materials have the advantages of wide sources, adjustable physical and chemical properties, and excellent processability, such as polyester, polytetrafluoroethylene, polycaprolactone, polyurethane, etc. , polylactic acid and other synthetic polymers, but the material lacks biological activity, poor cell compatibility, does not degrade or the degradation products affect the tissue microenvironment. Natural polymers are widely favored in the research of tissue engineering materials due to their excellent biological ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61L27/22A61L27/50A61L27/58C07K14/435C07K1/14
CPCA61L27/227A61L27/50A61L27/58C07K14/43586A61L2430/16
Inventor 王建南许建梅裔洪根
Owner SUZHOU UNIV
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