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Primer, kit and detection method for detecting vibrio parahaemolyticus

A hemolytic Vibrio and detection method technology, applied in the field of detection of Vibrio parahaemolyticus, can solve the problems of cumbersome primer design and achieve the effects of avoiding non-specificity, high sensitivity, and simple operation

Inactive Publication Date: 2020-11-03
HEBEI AGRICULTURAL UNIV.
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, LAMP amplification requires 4 to 6 primers, and the primer design is cumbersome, and the interaction between multiple primers may produce non-specific results

Method used

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  • Primer, kit and detection method for detecting vibrio parahaemolyticus
  • Primer, kit and detection method for detecting vibrio parahaemolyticus
  • Primer, kit and detection method for detecting vibrio parahaemolyticus

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Experimental program
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Effect test

Embodiment

[0041] 1.1 Test material

[0042] 1.1.1 Test strains

[0043] A total of 41 strains of bacteria were selected in this study, including 12 strains of Vibrio parahaemolyticus and 29 strains of non-parahaemolyticus Vibrio, as shown in Table 1:

[0044] Table 1 Bacteria used in research

[0045]

[0046] ATCC: America Type Culture Collection

[0047] CMCC: China Medical Culture Collection

[0048] CICC: China Center of Industrial Culture Collection

[0049] 1.1.2 Test medium

[0050] The medium used in this study mainly includes 3% sodium chloride alkaline peptone water, 3% sodium chloride tryptone soybean agar, nutrient broth, LB liquid medium and LB solid medium. All the above media were purchased from Beijing Land Bridge Technology Co., Ltd., and were used according to the instructions corresponding to the media.

[0051] 1.1.3 Test kits and reagents

[0052] Genomic DNA Extraction Kit, Gene Cloning T 3 Vector kit, DNA fragment recovery kit, Taq DNA polymerase, deoxyri...

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Abstract

The invention provides a primer, kit and detection method for detecting vibrio parahaemolyticus. According to the method provided by the invention, the specific primer is adopted, so that the method provided by the invention not only has the advantages of being high in specificity and high in sensitivity, but also is simple to operate and low in detection cost, and the reaction process can be completed only by virtue of a water bath kettle. SRCA can be subjected to an amplification reaction only by a pair of primers, so that non-specificity is avoided to a certain extent. In addition, the method does not need other equipment to detect the result, and the result can be obtained by directly observing fluorescence or precipitation with naked eyes. Therefore, the research can provide a certainreference for on-site screening of the vibrio parahaemolyticus and rapid specific detection of the basic level.

Description

technical field [0001] The invention relates to the technical field of detection of Vibrio parahaemolyticus, in particular to a primer, a kit and a detection method for detection of Vibrio parahaemolyticus. Background technique [0002] Vibrio parahaemolyticus (Vibrio parahaemolyticus) is a Gram-negative, facultative anaerobic halophilic bacterium widely distributed in coastal waters and seafood such as fish, shrimp and shellfish. important foodborne pathogens. The bacteria can cause food poisoning and cause acute diarrhea and other diseases, and in severe cases, it can lead to sepsis and even death. People with underlying chronic medical conditions, especially those with liver disease, are at increased risk of sepsis from V. parahaemolyticus infection, which can be life-threatening in severe cases. With the rapid development of the economy, seafood has become a new favorite on people's tables. At the same time, human food poisoning incidents caused by Vibrio parahaemolyt...

Claims

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Application Information

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IPC IPC(8): C12Q1/689C12Q1/04C12N15/11C12R1/63
CPCC12Q1/689
Inventor 张伟苑宁张蕴哲杨倩徐慧王晓凯
Owner HEBEI AGRICULTURAL UNIV.
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