A kind of glycoside compound derived from Sheng et al., its extraction method and its application
A compound and glycoside technology, applied in the field of glycoside compounds, can solve the problems that the stability and effectiveness of drugs need to be improved, the storage of raw and other extracts is troublesome, and microorganisms are easy to breed, and the effect of significantly reducing uric acid is achieved.
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Embodiment 1
[0083] Embodiment 1: a kind of raw and so on dry ointment:
[0084] Such as figure 2 and image 3 As shown, the dry paste of raw materials is prepared through the following steps:
[0085] (1) After cleaning and slicing 20kg of raw and other medicinal materials, add 120L of water to a 500L sandwich pot for the first decoction. The decoction temperature is 190°C and the decoction time is 90min. Stir once every 20min. , stirred at 15r / min for 1min; after decocting, filter for the first time (100 mesh sieve) to obtain medicinal residues I and medicinal liquid I;
[0086] (2) Add 80 L of water to the medicinal dregs I, and decoct for the second time. The decoction temperature is 190° C., the decoction time is 120 min, stirred once every 20 min, and stirred at 15 r / min for 1 min; after decoction, filter for the second time ( 100 mesh sieve), to obtain medicinal residues II and medicinal liquid II, and discard medicinal residues II;
[0087] (4) Mixing the medicinal liquid I an...
Embodiment 2
[0103] Embodiment 2: a kind of glycoside compound:
[0104] On the basis of Example 1, the glycoside compound was prepared by using the raw and other dry paste obtained, the specific steps are as follows:
[0105] 1) Take the raw raw paste described in Example 1, add 10 times the amount of an aqueous solution containing 65% ethanol and 1% glycerol, mix well, heat up to 85°C, extract 3 times, each time for 3h, and combine the extracts Concentrate under reduced pressure until there is no alcohol smell, and then sequentially extract with petroleum ether (60-90°C), chloroform, ethyl acetate, and water-saturated n-butanol;
[0106] 2) Take the part of ethyl acetate in step 1), separate it by column chromatography on silica gel (200-300 mesh), and elute with chloroform-methanol-water gradient, with volume ratios of 9:1:1 and 8:2: 1. The mixed solution of 7:3:1, 6:4:1, and 5:5:1 is eluted, and the eluted fractions 1 to 5 are collected correspondingly;
[0107] 3) Take fraction 5 (t...
Embodiment 3～12
[0111] Embodiment 3~12: a kind of glycoside compound:
[0112] On the basis of Example 1, the glycoside compound was prepared by using the raw and other dry paste obtained, the specific steps are as follows:
[0113] 1) Take the raw and other dry extracts described in Example 1, add 10 times the amount of aqueous solution, mix uniformly and then heat up to 85 ° C, extract 3 times, each time for 3 hours, combine the extracts, concentrate under reduced pressure, and then use petroleum ether ( 60~90℃), chloroform, ethyl acetate, and water-saturated n-butanol for extraction;
[0114] 2) with embodiment 2;
[0115] 3) with embodiment 2;
[0116] 4) with embodiment 2;
[0117] 5) Same as Example 2, finally separate and obtain the glycoside compound; wherein
[0118] The components of the aqueous solution of step 1) are specifically shown in Table 2.
[0119] The alcohol extraction solution composition in table 2, embodiment 2~12
[0120]
[0121] Statistical embodiment 2~12...
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