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Preparation method of fermented protein feed and fermented protein feed

A technology of fermented protein feed and fermented material, which is applied in the field of biological fermented feed, can solve the problem of acid-soluble protein content, protein solubility, removal of toxic and anti-nutritional components, unsatisfactory amino acid balance and palatability, difficulty in adapting to the strong demand for protein feed, fermentation The temperature is difficult to control and other problems, to achieve the effect of inhibiting the growth and reproduction of miscellaneous bacteria, improving the palatability of feed, and increasing the content of acid-soluble protein

Pending Publication Date: 2020-11-10
SICHUAN UNIVERSITY OF SCIENCE AND ENGINEERING
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Problems solved by technology

Therefore, there are problems such as single raw material, mostly lactic acid bacteria, high content of miscellaneous bacteria during fermentation, difficult to control fermentation temperature, high fermentation failure rate, only microbial fermentation without enzymatic hydrolysis synergy, etc.
The obtained fermented protein feed is not ideal in terms of acid-soluble protein content, protein solubility, removal of toxic and anti-nutritional components of raw materials, amino acid balance, and palatability, and there are unavoidable resource and price problems, so it is difficult to satisfy The nutritional requirements of full-price compound feed production or animal breeding are also difficult to meet the strong demand for protein feed in the rapid development of animal husbandry

Method used

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preparation example Construction

[0026] The invention provides a kind of preparation method of fermented protein feed, comprises the following steps:

[0027] 1) protein raw material, water, Tween 80 and peracetic acid solution are mixed, obtain mixed material; Described protein raw material comprises rapeseed meal, soybean meal and corn gluten meal; The quality of described rapeseed meal, soybean meal and corn gluten meal The ratio is (70~80):(10~30):(2~5);

[0028] 2) Inoculate Aspergillus oryzae in the mixed material obtained in the step 1), and perform solid-state aerobic fermentation to obtain a fermented material; the temperature of the solid-state aerobic fermentation is 28-32°C; the time of the solid-state aerobic fermentation 32~36h;

[0029] 3) After mixing the fermented material obtained in step 2) with Enterococcus faecium culture solution and water, carry out anaerobic fermentation and enzymolysis to obtain fermented protein feed; the temperature of the anaerobic fermentation and enzymolysis is ...

Embodiment 1

[0055] 1) Weigh 100kg of 40°C warm water, 800.5kg of Tween, 1.0kg of 16% peracetic acid solution (16% by mass), 80kg of rapeseed meal, 15kg of soybean meal and 5kg of corn gluten powder. Warm water, Tween 80, and peracetic acid solution were mixed evenly, and then mixed with the protein material, and left to moisten the material for 50 minutes to obtain the mixed material;

[0056] 2) Insert Aspergillus oryzae into the mixed material according to the inoculation amount of 2.5%, fully mix, control the thickness of the material to 30cm, carry out solid-state aerobic fermentation, the fermentation temperature is 28 ° C, ferment for 36 hours, and turn the material once every 8 hours during the period to obtain fermentation material;

[0057] 3) Fermented material and Enterococcus faecium culture solution (bacterial content of Enterococcus faecium ≥ 3.5×10 9 cfu / ml) according to the mass ratio of 100:0.5, after fully mixing, control the water content in the fermentation material t...

Embodiment 2

[0059] 1) Weigh 100kg of 60°C warm water, 801.5kg of Tween, 0.88kg of 16% peracetic acid solution (mass percentage content is 16%), 70kg of rapeseed meal, 28kg of soybean meal, and 2kg of corn gluten powder. Warm water was mixed with Tween 80 and peracetic acid solution, then mixed with protein raw material, and left to moisten the material for 20 minutes to obtain the mixed material;

[0060] 2) Insert Aspergillus oryzae into the mixed material according to the inoculation amount of 3.0%, fully mix, control the thickness of the material to 20cm, carry out solid-state aerobic fermentation, the fermentation temperature is 32°C, ferment for 32h, and turn the material once every 12h during the period to obtain fermentation material;

[0061] 3) Fermented material and bacterial culture solution (bacterial content of Enterococcus faecium ≥ 3.5×10 9 cfu / ml) according to the mass ratio of 100:1, after fully mixing, control the water content of the fermentation material to 50%, carry...

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Abstract

The invention provides a preparation method of a fermented protein feed and the fermented protein feed, and belongs to the technical field of biological fermented feeds. The preparation method comprises the following steps: 1) mixing a protein raw material with water, Tween 80 and a peroxyacetic acid solution to obtain a mixed material; 2) inoculating the mixed material obtained in the step 1) with aspergillus oryzae, and performing solid-state aerobic fermentation to obtain a fermented material; and 3) mixing the fermented material obtained in the step 2) with an enterococcus faecium culturesolution and water, and performing anaerobic fermentation and enzymolysis to obtain the fermented protein feed. Experimental results show that the preparation method of the fermented protein feed hasthe effects of reducing the contents of toxic, harmful and anti-nutritional ingredients in protein raw materials, increasing the content of acid-soluble protein, increasing the solubility of protein,improving and regulating amino acid balance and improving the palatability of the feed, so that the nutritional requirements for production of complete formula feeds or breeding of animals can be better met.

Description

technical field [0001] The invention belongs to the technical field of biological fermented feed, and in particular relates to a preparation method of fermented protein feed and fermented protein feed. Background technique [0002] Soybean meal is rich in protein. Although it contains various anti-nutritional factors, due to the balanced proportion of various essential amino acids and high content of lysine, it is easy for animals to digest and absorb. It also has good palatability, good color, and is easy to eat. eaten by livestock. Therefore, soybean meal, as a high-quality vegetable protein, is widely used in the feed industry and occupies a very important position. However, with the rapid development of animal husbandry, the shortage of soybean meal consumption is increasing day by day, and the price remains high, and it must rely on a large amount of imports to meet the demand. Therefore, it is imminent to seek new high-quality protein resources to alleviate the huge ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23K10/12A23K10/14A23K10/37A23K20/147
CPCA23K10/12A23K10/14A23K10/37A23K20/147Y02P60/87
Inventor 刘军李丽温雪瓶
Owner SICHUAN UNIVERSITY OF SCIENCE AND ENGINEERING
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