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Preparation method of oat beta-glucan

The technology of glucan and oat is applied in the field of preparation of oat beta-glucan, and can solve the problems of affecting the dissolution and purity of beta-glucan, high extraction cost of beta-glucan, restricting development and application, and the like, Achieve superior chemical activity, ensure product purity, and improve extraction efficiency

Inactive Publication Date: 2020-11-24
广州贝塔健康生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] In the prior art, oat bran is often used to extract β-glucan. In the extraction, starch and protein are mostly hydrolyzed by enzymatic hydrolysis, and then β-glucan is separated from the filtrate. In the process of polysaccharides, since oat bran itself has certain oils and other substances, which will affect the dissolution and purity of β-glucan, it is necessary to add a certain amount of organic solvent or ethanol to dissolve other substances in the extraction process. Then purify, but in the prior art, most of them are aimed at the removal and purification of starch and cellulose, and some organic solvents or ethanol and other substances will remain in the purified β-glucan, resulting in β-glucan The extraction cost of β-glucan is high, the purity is limited and the extraction rate is not good, and the application in different fields has certain requirements on the molecular weight of β-glucan. Seriously restrict its development and application

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] (1) After dehydrating and drying the oat grains, crush them to 100 mesh, separate and obtain oat bran, and then dry the oat bran with 20kW microwave for 10min;

[0034] (2) The oat bran in step (1) is subjected to supercritical carbon dioxide extraction at a pressure of 20 MPa and a temperature of 60° C., the extraction time is 5 hours, and the extraction flow rate is 4300 L / h;

[0035] (3) Disperse the oat bran treated in step (2) into clear water at a ratio of 1:10 to adjust the pH to 3, then insert a carbon rod electrode, and electrolyze at 15V for 15 minutes;

[0036] (4) Ultrasonicate the mixed solution after electrolysis at 60°C for 60 minutes, then adjust the temperature to 30°C and pH 6, add mixed enzymes for enzymolysis to obtain an enzymatic hydrolysis solution, wherein the mixed enzymes are α-amylase, glucoamylase and alkaline Protease, based on the weight of oat bran, the addition of the α-amylase is 0.1wt%, the glucoamylase is 0.5wt%, and the alkaline prote...

Embodiment 2

[0043] (1) After dehydrating and drying the oat grains, crush them to 100 mesh, separate and obtain oat bran, and then dry the oat bran with 40kW microwave for 8min;

[0044] (2) The oat bran in step (1) is subjected to supercritical carbon dioxide extraction at a pressure of 35 MPa and a temperature of 50° C., the extraction time is 5 hours, and the extraction flow rate is 3500 L / h;

[0045] (3) Disperse the oat bran treated in step (2) into clear water at a ratio of 1:20 to adjust the pH to 4, then insert a carbon rod electrode, and electrolyze at 20V for 10 minutes;

[0046] (4) Ultrasonicate the mixed solution after electrolysis at 50°C for 100 minutes, then adjust the temperature to 30°C and pH 7, add a mixed enzyme for enzymolysis to obtain an enzymatic hydrolysis solution, wherein the mixed enzyme is α-amylase, glucoamylase and alkaline Protease, based on the weight of oat bran, the addition of the α-amylase is 0.2wt%, the glucoamylase is 1.8wt%, and the alkaline protea...

Embodiment 3

[0053] (1) After dehydrating and drying the oat grains, crush them to 150 mesh, separate and obtain oat bran, and then dry the oat bran with 60kW microwave for 5min;

[0054] (2) The oat bran in step (1) is subjected to supercritical carbon dioxide extraction at a pressure of 50 MPa and a temperature of 40° C., the extraction time is 3 hours, and the extraction flow rate is 2200 L / h;

[0055] (3) Disperse the oat bran treated in step (2) into clear water at a ratio of 1:25 to adjust the pH to 4, then insert a carbon rod electrode, and electrolyze at 20V for 15 minutes;

[0056] (4) Ultrasonicate the mixed solution after electrolysis at 40°C for 150 minutes, then adjust the temperature to 40°C and pH8, add mixed enzymes for enzymolysis to obtain an enzymatic solution, wherein the mixed enzymes are α-amylase, glucoamylase and alkaline Protease, based on the weight of oat bran, the addition of the α-amylase is 0.3wt%, the glucoamylase is 3wt%, and the alkaline protease is 8wt%; ...

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Abstract

The invention discloses a preparation method of oat beta-glucan. The preparation method comprises the steps of performing degreasing treatment on raw materials to enable subsequent effective components to be dissolve out and reduce impurities, performing water extraction, and performing electrolysis treatment on a mixed solution to promote separation and dissolution of beta-glucan in oat bran; after electrolytic treatment, performing ultrasonic treatment and enzymolysis treatment to promote complete release of the beta-glucan, so as to effectively increase the extraction rate of the beta-glucan; and removing the impurities through isoelectric precipitation, flocculent precipitation and alcohol precipitation in the extraction process to ensure the purity of the product, and finally, obtaining products with different molecular weights by series separation of separation membranes with different specifications. The preparation method is simple, the requirements of various fields are met, finally, the chemical structure of the beta-glucan can be prevented from being damaged to the maximum extent in a vacuum drying mode, the excellent chemical activity of the beta-glucan is maintained, and the preparation method has high practical application value.

Description

technical field [0001] The invention relates to the technical field of fine chemicals, in particular to a preparation method of oat beta-glucan. Background technique [0002] Compared with other grains, oatmeal has comprehensive and high nutritional value. It ranks first in the five major nutritional indicators such as protein, fat, vitamins, mineral elements, and cellulose. Studies have shown that oats contain a large amount of dietary fiber, and the water-soluble dietary fiber β-glucan has high application value. β-glucan is a non-starch viscous polysaccharide composed of glucopyranose units connected by 1-3 and 1-4 glycosidic bonds. It has special physiological functions such as lowering cholesterol, regulating blood sugar, improving immunity, and preventing constipation. , improving the intestinal microenvironment, cosmetic intermediates, etc., widely used in the fields of medicine, health food and cosmetics. [0003] In the prior art, oat bran is often used to extrac...

Claims

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Application Information

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IPC IPC(8): C08B37/02
CPCC08B37/0003C08B37/0024Y02P20/54
Inventor 姚灿辉陆青蓉
Owner 广州贝塔健康生物科技有限公司
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