A kind of halotolerant bacillus sy1836 and its application
The technology of bacillus halide and inoculum is applied in the field of microorganisms, which can solve the problems of soil salinization and groundwater pollution, decrease in utilization rate of pesticides, decrease in quality of peppers, etc., achieve good biological control effect, increase plant height, and reduce disease conditions. The effect of the index
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Embodiment 1
[0026] This example provides a process for isolating and purifying Bacillus halotolerans SY1836 from soil, specifically as follows:
[0027]Soil samples were collected from the salinized plot in Shuiyuan Town, Jinchang City, Gansu Province. Randomly select 5 points in the sampling plot, take 0-5cm salinized soil, bring it back in a sealed bag after mixing, remove soil clods and plant residues with a 0.2cm mesh sieve, and air dry at room temperature. Weigh 10g of soil sample into a conical flask filled with 90mL sterilized water, shake it for 10min, and make 10 -1 Concentration of soil suspension, let it stand until the soil particles settle, then draw 1mL supernatant and transfer it into a test tube filled with 9mL sterilized water to make 10 -2 Concentration of the soil suspension, followed by serial dilution to make the final concentration is 10 -5 soil suspension. choose 10 -3 , 10 -4 and 10 -5 200 μL of each of the suspensions of the three concentrations was drawn, a...
Embodiment 2
[0034] In this example, substances related to disease prevention and growth promotion of Bacillus halotolerans (Bacillus halotolerans) SY1836 are detected, and the specific method is as follows:
[0035] 1.1 Production of siderophore
[0036] Pick the strain and inoculate it on the CAS medium, place it in a 30°C incubator and cultivate it for 14 days, observe whether there is a yellow-orange circle, if there is, it means that the strain has the ability to produce siderophiles.
[0037] 1.2 Production of amylase
[0038] Pick the strain and inoculate it on the NA medium containing 1% soluble starch, culture it in a 30°C incubator for 5 days, observe whether there is a transparent circle, if there is, it means that the strain has the ability to produce amylase.
[0039] 1.3 Production of protease
[0040] Pick the strain and inoculate it on a casein yeast medium containing 7% skimmed milk powder (casein 5g, yeast extract 2.5g, glucose 1g, water 1000mL), place it in an incubato...
Embodiment 3
[0061] This embodiment further detects the greenhouse disease prevention and growth promotion effect of Bacillus halotolerans (Bacillus halotolerans) SY1836, the specific method is as follows:
[0062] 2.1 Preparation of Bacillus halotolerans SY1836 biocontrol agent
[0063] Bacillus halotolerans (Bacillus halotolerans) SY1836 was inoculated on LB solid medium for activation, then transferred to a 100mL Erlenmeyer flask containing 50mL of LB liquid medium, and cultured at 28°C for 24h with shaking at 150rpm to obtain seed liquid. The seed solution was inoculated in the fermentation medium (1 / 2PDB+1 / 2LB) at a ratio of 1:100, and cultured at 28°C with shaking at 150rpm for 24h.
[0064] 2.2 Determination of the seedling tray control effect of Bacillus halotolerans SY1836 on pepper blight
[0065] The seeds of pepper variety "Changfeng" were surface-sterilized in 0.1% sodium hypochlorite for 20 min, and then washed four times with sterile water. Seeds were sown in 50-well seedl...
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