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Biological enhancement application method of sulfate reducing bacteria

A bio-augmentation and sulfate technology, applied in the field of environmental microorganisms, can solve problems such as pollution, irritating odor, and difficulty in the growth of sulfate-reducing bacteria, and achieve the effect of reducing environmental pollution and reducing content

Inactive Publication Date: 2020-11-27
武汉谦豫科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] However, there are obvious defects in the actual application of monosulfate-reducing bacteria, such as the difficulty in the growth of immobilized sulfate-reducing bacteria and the production of pungent odors during application (H 2 S), causing secondary potential pollution, etc.

Method used

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  • Biological enhancement application method of sulfate reducing bacteria
  • Biological enhancement application method of sulfate reducing bacteria

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] A method for applying sulfate-reducing bacteria bioaugmentation, the steps are as follows:

[0037] (1) Prepare bio-augmented medium: add 1.5g KH to 1L distilled water 2 PO 4 , 2.5 g / L NaCl, 2.0g / L sodium lactate, 0.5g / L peptone, 1.5g / L Na 2 SO 4 , 0.05 g / L MgCl 2 ·6H 2 O, 0.05g / L CaCl 2 . Add 1mol / L NaOH to adjust the pH to 7.0. The prepared culture was sterilized at 121°C for 20 minutes, and cooled for later use.

[0038] (2) Take 0.5g glucose, 1.0g NH 4 Cl, 0.1g ascorbic acid, 1.0g urea sterilized by UV alone for 30min, 20mgCdCl 2 Add to the above-prepared 1L solution.

[0039] (3) Take the culture solution of sulfate-reducing bacteria and urease-producing strains at a volume ratio of 1:1, and add the two microorganisms into the bio-augmented culture solution at the same time. .

[0040] (4) The mixed bacterial solution was cultured in a constant temperature oscillator at 30-35°C and 60-85 r / min for 24 hours, and the Cd in the medium was determined by fla...

Embodiment 2

[0042] (1) Prepare bio-augmented medium: add 1.5g KH to 1L distilled water 2 PO 4 , 2.5 g / L NaCl, 2.0g / L sodium lactate, 0.5g / L peptone, 1.5g / L Na 2 SO 4 , 0.05 g / L MgCl 2 ·6H 2 O, 0.05g / L CaCl 2 . Add 1mol / L NaOH to adjust the pH to 7.0. The prepared culture was sterilized at 121°C for 20 minutes, and cooled for later use.

[0043] (2) Take 0.5g glucose, 1.0g NH 4 Cl, 0.1g ascorbic acid, 1.0g urea sterilized by UV alone for 30min, 20mgCdCl 2 Add to the above-prepared 1L solution.

[0044] (3) Take the culture solution of sulfate-reducing bacteria and urease-producing strains at a volume ratio of 1:1, first add the urease-producing strains for 3 hours, and then add sulfate-reducing bacteria. Closed culture.

[0045] (4) The mixed bacterial solution was cultured in a constant temperature oscillator at 30-35°C and 60-85 r / min for 24 hours, and the Cd in the medium was determined by flame atomic absorption spectrophotometry. 2+ content.

Embodiment 3

[0047] (1) Prepare bio-augmented medium: add 1.5g KH to 1L distilled water 2 PO 4 , 2.5 g / L NaCl, 2.0g / L sodium lactate, 0.5g / L peptone, 1.5g / L Na 2 SO 4 , 0.05 g / L MgCl 2 ·6H 2 O, 0.05g / L CaCl 2 . Add 1mol / L NaOH to adjust the pH to 7.0. The prepared culture was sterilized at 121°C for 20 minutes, and cooled for later use.

[0048] (2) Take 0.5g glucose, 1.0g NH 4 Cl, 0.1g ascorbic acid, 1.0g urea sterilized by UV alone for 30min, 20mgCdCl 2 Add to the above-prepared 1L solution.

[0049] (3) Take the culture solution of sulfate-reducing bacteria and urease-producing strains at a volume ratio of 1:1, first add sulfate-reducing bacteria for 3 hours, and then add urease-producing strains. Closed culture.

[0050] (4) The mixed bacterial solution was cultured in a constant temperature oscillator at 30-35°C and 60-85 r / min for 24 hours, and the Cd in the medium was determined by flame atomic absorption spectrophotometry. 2+ content.

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Abstract

The invention discloses a biological enhancement application method of sulfate reducing bacteria. The method is characterized in that urease-producing strains are adopted to enhance the heavy metal immobilization capacity, a mixed bacteria culture solution is added into cadmium-containing wastewater, and closed culture is performed to obtain treated CdS precipitate. The urease-producing strain adopted by the invention has a partial symbiotic relationship with sulfate reducing bacteria, and consumes O2 to provide an expected anaerobic environment for the SRB strain while jointly participating in microbial precipitation to improve the heavy metal immobilization efficiency. Meanwhile, pollution of H2S in a cultured bacterial solution to the environment is reduced while sulfur ion S2-oxidizingbacteria growth is inhibited. In a waste liquid containing 20mg / L CdCl2, the cadmium removal rate reaches 99.0%, and the method has a good cadmium removal effect and can be widely applied to treatment of heavy metal-containing water sources and soil.

Description

Technical field: [0001] The invention belongs to the technical field of environmental microorganisms, and in particular relates to a method for the bioaugmentation application of sulfate-reducing bacteria. Background technique: [0002] At present, with the rapid development of society and economy, the enrichment of heavy metal elements in the environment is increasing, and human activities such as mineral resource development, metal processing and smelting, chemical production, factory discharge and sewage irrigation have gradually evolved into the main source of heavy metal pollution. Excessive discharge of heavy metals into the environment will not only lead to a decline in soil production capacity, but also pose a threat to human health. For example, Cd accumulates in the human body through the food chain, causing damage to the human immune system, urinary system, bones, nervous system, reproductive system, etc. At the same time, Cd also has strong carcinogenic, teratoge...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C02F3/34B09C1/10C12R1/01C12R1/63C02F101/20
CPCC12N1/20C02F3/34B09C1/10C02F2101/20
Inventor 张献波郑连爽康杰卿
Owner 武汉谦豫科技有限公司
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