Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Kit for detecting adenovirus IgM antibody by magnetic particle chemiluminiscence method and preparation method of kit

A kit and magnetic particle technology, applied in the directions of biochemical equipment and methods, chemical instruments and methods, and botanical equipment and methods, can solve the problems of narrow linear range, low sensitivity, false positives, etc., and achieve improved sensitivity and linearity. range, increased sensitivity, increased specificity

Active Publication Date: 2020-11-27
BEIJING BEIER BIOENG
View PDF15 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The colloidal gold method is generally operated manually, and finally qualitatively judged by naked eyes. This technique has low sensitivity, is prone to false positives, and is greatly influenced by human subjective factors.
The sensitivity of enzyme-linked immunosorbent assay is improved compared with colloidal gold method, but it is still low, and the linear range is narrow, and the reaction time is also long, which still cannot meet the clinical application well.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Kit for detecting adenovirus IgM antibody by magnetic particle chemiluminiscence method and preparation method of kit
  • Kit for detecting adenovirus IgM antibody by magnetic particle chemiluminiscence method and preparation method of kit
  • Kit for detecting adenovirus IgM antibody by magnetic particle chemiluminiscence method and preparation method of kit

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0072] 1. A kit for detecting adenovirus IgM antibody by magnetic particle chemiluminescence method, said kit comprising:

[0073] Enzyme label working solution, magnetic bead suspension, sample diluent, calibrator and luminescent substrate solution;

[0074] Wherein, the enzyme-labeled working solution includes the following final concentration components: bovine serum albumin (BSA) 2.5g / L, newborn calf serum 100mL / L, sodium chloride 8.0g / L, disodium hydrogen phosphate 5.8g / L , sodium dihydrogen phosphate 0.593g / L and horseradish peroxidase-labeled adenovirus antigen 1μg / mL;

[0075] The magnetic bead suspension includes the following final concentration components: 1 mg / mL of magnetic particles coupled with mouse anti-human IgM antibody (based on the mass of magnetic particles), wherein 1 mg of magnetic particles is coupled with 40 μg of mouse anti-human IgM antibody; the magnetic particles are superparamagnetic particles; every 100mL magnetic particles are activated with 1...

Embodiment 2

[0095] 1. A kit for detecting adenovirus IgM antibody by magnetic particle chemiluminescence method, said kit is the same as Example 1, the difference is that:

[0096] The adenovirus antigen is recombinant antigen B.

[0097] 2. The preparation method of the kit is the same as in Example 1.

Embodiment 3

[0099] 1. A kit for detecting adenovirus IgM antibody by magnetic particle chemiluminescence method, said kit is the same as Example 1, the difference is that:

[0100] The adenovirus antigen is recombinant antigen C.

[0101] 2. The preparation method of the kit is the same as in Example 1.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
concentrationaaaaaaaaaa
Login to View More

Abstract

The embodiment of the invention relates to the field of immunodetection, and particularly relates to a kit for detecting an adenovirus IgM antibody by a magnetic particle chemiluminiscence method anda preparation method of the kit. The kit comprises a calibration product, a sample diluent, an enzyme-labeled working solution, a magnetic bead suspension and a luminous substrate solution, wherein the enzyme-labeled working solution comprises a horse radish peroxidase labeled adenovirus antigen, and the magnetic bead suspension comprises magnetic particles coupled with a mouse anti-human IgM antibody; the adenovirus antigen comprises one or more of a recombinant antigen A, a recombinant antigen B or a recombinant antigen C; the amino acid sequence of the recombinant antigen A comprises a sequence as shown in SEQ ID NO.1; the amino acid sequence of the recombinant antigen B comprises a sequence as shown in SEQ ID NO.2; and the amino acid sequence of the recombinant antigen C comprises a sequence shown as SEQ ID NO. 3. According to the kit provided by the embodiment of the invention, the sensitivity and the linear range are improved, and the specificity and the accuracy are good.

Description

technical field [0001] The invention relates to the field of immune detection, in particular to a kit for detecting adenovirus IgM antibody by magnetic particle chemiluminescence method and a preparation method thereof. Background technique [0002] Adenovirus (adenovirus, Adv) belongs to the Adenoviridae family and has a double-helix DNA genome. There is no liposome sheath around the capsid of the adenovirus, but acts directly on the host cell. There are 47 subtypes of adenoviruses that are pathogenic to humans, and they are one of the important pathogens that cause respiratory and digestive tract infections in humans. Early prevention of adenovirus is of great significance. [0003] At present, adenovirus diagnosis for clinical and scientific research mainly includes etiology diagnosis, molecular biology diagnosis and immunology diagnosis. Etiological diagnosis mainly uses the method of cell culture to separate and identify pathogens in clinical urine, blood and other s...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/569G01N33/543G01N33/535G01N21/76C07K14/075C12N15/34
CPCG01N33/56983G01N33/54326G01N33/535G01N21/76C07K14/005G01N2469/20G01N2333/075C12N2710/10022
Inventor 徐悦
Owner BEIJING BEIER BIOENG
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com