A kind of nano-sustained-release preparation and its preparation method and its application in the preparation of medicines for the treatment of mitochondrial dysfunction diseases

A nano-sustained-release and nano-particle technology, applied in the field of nano-materials, can solve problems such as inability to cure, limit therapeutic effect, and poor ability of cyclosporin A to penetrate the blood-brain barrier

Active Publication Date: 2021-07-23
NANJING UNIV
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Problems solved by technology

[0004] The current drugs for the treatment of diseases related to mitochondrial dysfunction include cyclosporine A (CSA) and cyclosporine A analogs, mitochondrial coenzyme Q 10 , α-lipoic acid, etc., but the ability of cyclosporin A to penetrate the blood-brain barrier is poor, which limits its therapeutic effect; mitochondrial coenzyme Q 10 It can clear the ROS level of reactive oxygen species in the mitochondria, but it can only alleviate the disease, but not cure it; α-lipoic acid is a mitochondrial antioxidant, but it also has defects that can only alleviate but not cure

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  • A kind of nano-sustained-release preparation and its preparation method and its application in the preparation of medicines for the treatment of mitochondrial dysfunction diseases
  • A kind of nano-sustained-release preparation and its preparation method and its application in the preparation of medicines for the treatment of mitochondrial dysfunction diseases
  • A kind of nano-sustained-release preparation and its preparation method and its application in the preparation of medicines for the treatment of mitochondrial dysfunction diseases

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preparation example Construction

[0044] The present invention provides the preparation method of above-mentioned nano-sustained-release preparation, comprises the following steps:

[0045] (1) providing an alcohol dispersion of dextran-modified cerium oxide nanoparticles;

[0046] (2) Mix silk fibroin with lithium bromide solution, carry out hydrolysis and first dialysis successively, obtain silk fibroin solution in the dialysis bag;

[0047] (3) mixing the alcohol dispersion of the dextran-modified cerium oxide nanoparticles with the silk fibroin solution, and performing ultrasonication to obtain a suspension;

[0048] (4) Freeze storage, thawing and second dialysis of the suspension to obtain a sustained-release nano preparation;

[0049] The steps (1) and (2) are not limited by time sequence.

[0050] The present invention provides an alcohol dispersion of dextran-modified cerium oxide nanoparticles. The present invention has no special requirements for the preparation method of the dextran-modified cer...

Embodiment 1

[0063] (1) After the natural silk fiber (purchased from Nantong Xinyuan Co., Ltd.) is desericinized with alkali, the remaining cotton-like bulky solid is silk fibroin; take 20g of silk fibroin, and then use 50mL, 9M lithium bromide (purchased from Aladdin Company) hydrolysis, the hydrolysis time is 240min, and the hydrolysis temperature is 60 ℃. Remove impurity molecules through a dialysis bag with a molecular weight cut-off of 12,000 to 14,000 to obtain a silk fibroin solution;

[0064] (2) Dextran-coated Cerium Oxide Nanoparticles: A Computed Tomography ContrastAgent for Imaging the Gastrointestinal Tract and Inflammatory Bowel Disease with a diameter of 1-10nm dextran-modified cerium oxide nanoparticles (for the preparation method refer to "Dextran-coated Cerium Oxide Nanoparticles: A Computed Tomography Contrast Agent for Imaging the Gastrointestinal Tract and Inflammatory Bowel Disease") and absolute ethanol The solutions were mixed to obtain an alcohol dispersion of ceri...

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Abstract

The invention provides a nano sustained-release preparation, which belongs to the technical field of nano materials. The nano sustained-release preparation provided by the present invention comprises dextran-modified cerium oxide nanoparticles and silk fibroin coated on the outer layer of the dextran-modified cerium oxide nanoparticles. In the present invention, cerium oxide nanoparticles coated with dextran have good water-soluble dispersibility, and have excellent antioxidant activity and oxygen-carrying capacity, which can significantly improve neuron loss caused by hypoxia and oxidative damage, and restore mitochondria The morphology and structure of silk fibroin have good biocompatibility, and the use of silk fibroin to coat dextran-modified cerium oxide nanoparticles can achieve the effect of slow release of nanoparticles, avoiding the shortcomings of instantaneously high drug concentration and rapid metabolism; At the same time, silk fibroin is biodegradable, and its degradation products are safe and pollution-free.

Description

technical field [0001] The invention relates to the technical field of nanometer materials, in particular to a nanometer sustained-release preparation, a preparation method thereof and an application in preparation of medicines for treating mitochondrial dysfunction diseases. Background technique [0002] Mitochondria is an organelle covered by the outer mitochondrial membrane (OMM) and the inner mitochondrial membrane (IMM), and is the site of oxidative metabolism in eukaryotes. Mitochondria is one of the organelles most sensitive to various damages. Inhibition of the respiratory chain, reduction of enzyme activity, damage to mitochondrial DNA and damage to mitochondrial membranes can all cause mitochondrial dysfunction (mitochondrial dysfunction, MD). MD generally manifests as changes in the number, size, and shape of mitochondria. Quantitatively, mitochondria will hyperplasia, which is an adaptive response to chronic non-specific cell damage. In the case of acute injury o...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K9/52A61K47/42A61K47/36A61K33/244A61P25/00A61P25/28A61P25/16A61P21/00
CPCA61K9/5161A61K9/5169A61K9/5192A61P21/00A61P25/00A61P25/16A61P25/28A61K33/244
Inventor 李宽钰徐莉
Owner NANJING UNIV
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