Extraction and separation method and application of homogeneous morinda citrifolia polysaccharides
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A polysaccharide extraction and separation method technology, which is applied in the direction of medical preparations containing active ingredients, pharmaceutical formulas, organic active ingredients, etc., can solve the problems of long time consumption, low extraction rate, and easy destruction of ingredients, so as to shorten the extraction time, The effect of improving extraction efficiency
Active Publication Date: 2020-12-01
哈尔滨镀淀医药科技有限公司
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[0004] The present invention aims to solve the technical problems that the existing noni polysaccharide extraction method has components that are easily destroyed, takes a long time, has a low extraction rate, and has unclear pharmacological effects of noni polysaccharide, and provides noni polysaccharide and its extraction and separation Methods and Applications
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specific Embodiment approach 1
[0036] Specific implementation mode one: the noni fruit homogeneous polysaccharide extraction and separation method of this implementation mode is specifically carried out according to the following steps:
[0037] 1. Remove impurities from the surface of the dried Noni fruit, crush it, and sieve it to obtain the Noni fruit coarse powder;
[0038] 2. Soak noni fruit coarse powder in distilled water, then apply a voltage for flash extraction, and control the voltage at 100-140V to obtain the extract;
[0039] 3. Cool the extract to room temperature, then filter to obtain the filtrate, and use a rotary evaporator to concentrate the filtrate to obtain a concentrate;
[0040] 4. Add the ethanol solution to the concentrated solution, control the temperature at 4-5°C for alcohol precipitation for 24-25 hours, and then perform centrifugation to obtain the precipitate;
[0041] 5. Dissolve the precipitate in distilled water, then centrifuge, take the supernatant to concentrate, dry, ...
specific Embodiment approach 2
[0046] Specific embodiment 2: The difference between this embodiment and specific embodiment 1 is that the aperture of the sieve is 40 mesh when sieving in step 1. Others are the same as in the first embodiment.
specific Embodiment approach 3
[0047] Specific embodiment three: the difference between this embodiment and specific embodiment one or two is: the mass volume ratio of noni fruit coarse powder and distilled water is 1g: (20~60) mL in the step 2, soaks 6~7h. Others are the same as in the first embodiment.
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Abstract
The invention discloses an extraction and separation method and application of homogeneous morinda citrifolia polysaccharides, belonging to the field of extraction, separation and application of polysaccharides. The invention aims to solve the technical problems that components are easy to damage, consumed time is long, an extraction rate is low and the pharmacological action of the morinda citrifolia polysaccharides is not clear in conventional morinda citrifolia polysaccharide extraction methods. The method comprises the following steps: preparing coarse morinda citrifolia powder; carrying out flash extraction, concentration, alcohol precipitation, concentration and drying to obtain crude morinda citrifolia polysaccharides; carrying out deproteinization treatment and decoloration; and carrying out elution by adopting a DEAE-52 fiber column, and carrying out Sephadex G-75 gel column chromatography so as to obtain five kinds of homogeneous morinda citrifolia polysaccharides. Accordingto the invention, a morinda citrifolia polysaccharide extraction method is improved and optimized, effective components in morinda citrifolia can be reserved to the maximum extent by utilizing a flashextraction method, extraction time is remarkably shortened, and extraction efficiency is greatly improved. It is verified that the homogeneous morinda citrifolia polysaccharides have an inhibiting effect on human hepatoma carcinoma cells (HepG2). The homogeneous morinda citrifolia polysaccharides extracted by the method are applied as bioactive components for inhibiting human hepatoma carcinoma cells to preparation of antitumor drugs.
Description
technical field [0001] The invention relates to the field of extraction, separation and application of polysaccharides. Background technique [0002] Noni, scientific name Morinda citrifolia, also known as seashore Morinda citrifolia, Morinda citrifolia, Noni, is a shrub to small tree of Morinda citrifolia in the Rubiaceae family. Medicinal plants have a history of more than 2000 years. The fruit of noni fruit is green when immature, turns yellow when ripe, and finally brown. The medicinal properties of Noni fruit make it popular in my country, Polynesia and Hawaii. However, since the plant cell wall of Noni fruit is relatively strong, the main task of extracting plant polysaccharides is to break the cell wall. Therefore, the existing methods for extracting noni polysaccharides are often time-consuming, and the extraction rate is low, only between 6% and 8%, and the extraction method needs to be improved. [0003] At the same time, more and more domestic and foreign scho...
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