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Rice PAL1 gene as well as encoding protein and application thereof

A rice and protein technology, applied in the field of genetic engineering, can solve the problems of unknown specific models and insufficient number of genes, etc., and achieve the effect of increasing yield

Active Publication Date: 2020-12-01
INST OF CROP SCI CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, although some rice panicle development-related genes have been identified, the specific mode of action is still unknown, and the number of genes that have been reported to regulate panicle development is not abundant enough

Method used

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  • Rice PAL1 gene as well as encoding protein and application thereof
  • Rice PAL1 gene as well as encoding protein and application thereof
  • Rice PAL1 gene as well as encoding protein and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] Example 1 pal1 Mutant acquisition and phenotypic analysis

[0040] Mutagenesis of Japonica Rice Variety Huaidao 5 by 60Co-γ Radiation , to obtain a mutant with reduced plant height and shorter panicle length pal1 ( panic length 1 ). 20 wild-type and pal1 For the samples of mutants, statistical analysis was carried out on the plant height, panicle length, primary branch number, secondary branch number and panicle grain number. The results of the analysis showed that rice pal1 The mutant plant height was significantly reduced by 15.2% compared with the wild type ( figure 1 A, E). Compared with wild type, pal1 The panicle length of the mutant was significantly reduced by 25.6%, and the number of primary branches and secondary branches were also significantly reduced by 22.0% and 45.9%, respectively ( figure 1 B, C, D, F, G, H), which finally led to a significant decrease of 40.2% in the number of grains per panicle ( figure 1 I).

Embodiment 2

[0041] Embodiment 2 Rice PAL1 Gene acquisition

[0042] Will pal1 F 1 , F 1 F 2 To separate groups, use F 2 Populations for genetic analysis and gene mapping. to F 2 The analysis of the strains segregating generation traits showed that the segregation ratio of normal and mutant plants was 3:1, which indicated that the mutant traits were controlled by a pair of recessive genes.

[0043] with F 2 30 mutants of the japonica rice variety Nipponbare provided by NCBI (http: / / www.ncbi.nlm.nih.gov / ) were developed by comparing the whole genome sequence of the indica rice variety Dular sequenced in this experiment with the genome sequence of the japonica rice variety Nipponbare. A large number of Indel markers evenly distributed on the 12 chromosomes of rice were initially mapped, and the candidate gene was located between the R3-17 and R3-20 markers on chromosome 3 ( figure 2 A). In order to further fine-tune the candidate genes, continue to expand the F 2 Generation mapp...

Embodiment 3

[0050] Example 3 pCAMBIA1305.1::PAL1 vector transformed rice pal1 mutant

[0051] In order to conduct functional complementarity experiments, a PAL1 Functional complementation vector driven by the gene's own promoter. The 2525 bp before the translation initiation site ATG was selected as the promoter of the gene, and the promoter region and genomic DNA (excluding 3'UTR) were simultaneously amplified. The 5' end of the amplified fragment was introduced into the EcoRI site, and the 3' end was introduced into the PmlI site. The length of the PCR product was 8836 bp (including the homologous sequence on the vector). will eventually PAL1 A total of 8802 bp (shown in SEQ ID NO.2) of the self-promoter and the entire genome were connected together between the EcoRI and PmlI sites of the pCAMBIA1305.1 vector to form a reversion vector driven by the self-promoter ( image 3 ).

[0052] Transform the constructed complementary vector into Agrobacterium EHA105, and infect pal1 Mu...

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Abstract

The invention relates to the field of gene engineering, in particular to a rice PAL1 gene as well as an encoding protein and application thereof. The nucleotide sequence of the rice PAL1 gene is shownas SEQ ID NO.1, and the amino acid sequence of the rice PAL1 protein is shown as SEQ ID NO.4. Mutation of the gene leads to reduction of plant height and ear length of rice, and the number of first-stage branches, the number of second-stage branches and the number of ear grains are reduced. A transgenic technology finds that the PAL1 gene can restore the mutant ear type to a normal phenotype. According to the invention, the PAL1 gene with the function of regulating and controlling the ear length of the rice and the encoding protein of the PAL1 gene are discovered, and the ear type of the riceis an important character influencing the yield of the rice, so that the PAL1 gene is used for regulating and controlling the ear character of the rice, and the plant type is expected to be directionally designed to improve the yield of the rice.

Description

technical field [0001] The invention belongs to the field of genetic engineering, in particular, relates to rice PAL1 Genes and their encoded proteins and applications. Background technique [0002] Rice ( Oryza sativa L.) is one of the important food crops in my country, its yield and quality directly affect the food security and people's living standards in my country. Rice panicle shape has always been one of the important contents of rice genetics and breeding research, and is an important trait affecting rice yield. The rice panicle structure is a panicle, which consists of a main shaft, first-level branches, second-level branches and spikelets on the branches. According to the length of the cob, the density of the number of branches and spikelets, and whether it is erect or not, the panicle type of rice can be divided into long and short panicle, sparse dense panicle and curved straight panicle. [0003] In recent years, with the completion of rice genome sequenc...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/82C07K14/415C12N15/29
CPCC12N15/8261C07K14/415C12N9/88C12N15/8262Y02A40/146C12N15/8223
Inventor 李学勇淳雁赵金凤房静静袁守江
Owner INST OF CROP SCI CHINESE ACAD OF AGRI SCI
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