Biological antidote for relieving or treating harm of mycotoxin to cells

A technology of mycotoxins and antidotes, which is applied in the direction of anti-toxins, drug combinations, medical preparations containing active ingredients, etc., and can solve problems such as poor adsorption effect, difficult industrialization, and environmental pollution

Active Publication Date: 2020-12-04
固原市畜牧技术推广服务中心 +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, since the physical adsorption method is only effective for aflatoxins, and poor for other mycotoxins, the adsorbent not only adsorbs mycotoxins, but also absorbs nutrients such as vitamins and mineral elements, and there is also a desorption process. problem; thus, phased out in production
Chemical methods are mainly to treat mycotoxins through organic acid treatment, alkali treatment, oxidant treatment or other compounds to reduce or eliminate their toxicity by destroying their chemical structure; the disadvantage is that the process of using some chemical reagents will cause environmental pollution , causing damage to nutrients in the feed, resulting in nutrient loss, high production costs and difficult industrialization

Method used

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  • Biological antidote for relieving or treating harm of mycotoxin to cells
  • Biological antidote for relieving or treating harm of mycotoxin to cells
  • Biological antidote for relieving or treating harm of mycotoxin to cells

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] First, using different concentrations of aflatoxin B 1 (AFB 1 ), zearalenone (ZEA) and deoxynivalenol (DON) on the toxicity of piglet jejunum epithelial cells (IPEC-J2), the specific process is as follows:

[0042] Cell recovery: take out the frozen cells stored at -80°C, quickly put them into a 37°C constant temperature water bath and shake continuously, and the cells are completely dissolved in about 1min. Centrifuge for 5 minutes, remove the supernatant, add 1 mL of preheated high-glucose medium containing 10% FBS and 1% double antibody to resuspend the cells, then transfer 1 mL of the cell suspension into a cell culture bottle, and then add 4 mL of fresh culture medium. Gently make a cross to spread the cell suspension evenly on the wall of the bottle. 37°C, 5% CO 2 Cultured under certain conditions, the freshly recovered cells need to change the medium once after 24 hours, and the cells are subcultured when the cells grow to 80%-90% of the bottle wall.

[0043...

Embodiment 2

[0060] The inventor screened out a degrading AFB from more than a dozen microorganisms of the genus Bacillus, Saccharomyces, and Lactobacillus. 1 , ZEA, DON have better effects on Bacillus licheniformis, Bacillus coagulans and Clostridium butyricum. Among them, Bacillus licheniformis or Bacillus coagulans have been reported to relieve zearalenone (ZEA), aflatoxin B 1 (AFB 1 ) or deoxynivalenol (DON), but usually, the application of Bacillus licheniformis or Bacillus coagulans only targets one or two of the above three mycotoxins The degradation effect of synergistic virulence of mycotoxins has not been reported before. Clostridium butyricum has been reported to relieve DON, but its effect on ZEA, AFB 1 There is not much disclosure about the detoxification effect on the Internet.

[0061] The inventors first determined the degradation effects of Bacillus licheniformis, Bacillus coagulans and Clostridium butyricum on the combined toxicity of three mycotoxins:

[0062] Take ...

Embodiment 3

[0086] In Example 2, the degradation effects of different probiotics or combinations of probiotics on three mycotoxins were investigated. Then the inventors investigated the specific effects of the above probiotic combination on the cells after the joint treatment of the three mycotoxins:

[0087] IPEC-J2 cells in the logarithmic growth phase were taken, routinely digested and counted, and inoculated into a 96-well plate, 100 μL / well, and the number of cells per well was 1×10 4 , and then place the cell culture plate in CO 2 Cultivate in the incubator for 24 hours, discard the original culture solution, and add PBS buffer solution to wash again.

[0088] Add different concentrations of mycotoxins diluted with cell culture medium, and treat with probiotics and probiotics + chlorogenic acid combination respectively; among them, AFB in high damage group and low damage group 1 , the addition amount of ZEA and DON with reference to embodiment 1, the addition amount of chlorogenic...

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Abstract

The invention belongs to the technical field of a biological antidote, and particularly relates to a biological antidote for relieving or treating harm of mycotoxin to cells. The biological antidote consists of 1*105 to 1*107 CFU/mL of bacillus licheniformis, 1*105 to 1*107 CFU/mL of bacillus coagulans and 1*105 to 1*107 CFU/mL of clostridium butyricum; the bacillus licheniformis, the bacillus coagulans and the clostridium butyricum are purchased from Guangdong Microbial Culture Collection Center; the preservation number of the bacillus licheniformis is GDMCC1.11, the preservation number of the bacillus coagulans is GDMCC1.645, and the preservation number of the clostridium butyricum is GDMCC1.676; and the mycotoxin is aflatoxin B1, zearalenone and vomitoxin. Based on a total inventive concept, the invention further discloses application of chlorogenic acid and composite probiotics which are used as the biological antidote for relieving or treating harm of the mycotoxin. the Accordingto the invention, the chlorogenic acid, composite probiotics or a composition of chlorogenic acid and composite probiotics is used for relieving the damage of mycotoxin to cells, so that the combineddetoxification of aflatoxin B1, the zearalenone and vomitoxin is implemented.

Description

technical field [0001] The invention belongs to the technical field of biological antidotes, and in particular relates to a biological antidote for alleviating or treating the damage of mycotoxins to cells. Background technique [0002] Mycotoxins in feed mainly include aflatoxin B 1 (AFB 1 ), zearalenone (ZEA) and deoxynivalenol (DON), etc. AFB 1 It has obvious hepatotoxicity, carcinogenicity and teratogenicity to humans and animals, and reduces the immunity of humans and animals and the production performance of animals; therefore, it is designated as a class I carcinogen by the International Agency for Research on Cancer (IARC). ZEA has an estrogen-like effect, which can increase the estrogen level of female animals, thereby causing changes in the reproductive physiology and function and morphology of reproductive organs of animals, resulting in cytotoxicity and genotoxicity, and easily leading to reproductive disorders. DON has strong cytotoxicity and immunosuppressi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K35/742A61P1/00A61P39/02A61K31/216
CPCA61K35/742A61P1/00A61P39/02A61K31/216A61K2300/00
Inventor 尹清强王晓敏张国坪谢建亮杨春莲常娟王平党晓伟朱群刘超齐李茂龙张瑞
Owner 固原市畜牧技术推广服务中心
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