Method for quantitatively detecting short-chain fatty acid in human digestive tract
A technology for quantitative detection of short-chain fatty acids, applied in measuring devices, instruments, scientific instruments, etc., can solve problems affecting the accuracy and repeatability of the method, harmful to the environment and human health, and reduce the recovery rate, so as to achieve the accuracy of detection and high sensitivity, good precision and sensitivity, and improved recovery
Inactive Publication Date: 2020-12-04
HUAZHONG AGRI UNIV
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Problems solved by technology
In addition, derivatization of SCFAs is often also performed as a necessary pretreatment and different reagents have been used, although these pretreatments have produced good purification and higher response of flame ionization detectors, they are relatively time-consuming , may also reduce the recovery rate and affect the accuracy and repeatability of the method. In addition, in most of the above methods, a large number of organic solvents and reagents that are harmful to the environment and human health are used. A simple and environmentally friendly method is currently required to measure short chain fatty acids with small amounts of pretreatment
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[0043] see Figure 1-Figure 7 As shown, the present invention provides a method for quantitatively detecting short-chain fatty acids in human digestive tract, comprising the following steps:
[0044] Step 1: Prepare short-chain fatty acid standard solution and sample extract to be tested, and prepare short-chain fatty acid standard stock solution: Accurately weigh 240.0 mg of acetic acid standard, 296.0 mg of propionic acid, 352.4 mg of butyric acid, and 204.3 mg of valeric acid , isovaleric acid 204.3mg, isobutyric acid 88.1mg, hexanoic acid 58.1mg and heptanoic acid 19.5mg, then dissolve in 10mL three-distilled water, carry out ultrasonic heating and dissolve, as short-chain fatty acid standard stock solution one; Methods The short-chain fatty acid standard solutions with different concentrations were measured, the chromatograms and peak areas of the eight short-chain fatty acid standard solutions were recorded, linear regression analysis was carried out, and respective stan...
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Abstract
The invention discloses a method for quantitatively detecting short-chain fatty acid in the human digestive tract. The method comprises the following steps: measuring short-chain fatty acid standard solutions with different concentrations by GC detection, recording chromatograms and peak areas of the short-chain fatty acid standard solutions, carrying out linear regression analysis, and drawing astandard curve; and adding the short-chain fatty acid standard solutions with high, intermediate and low concentrations into an excrement suspention to obtain a mixed solution. According to the present invention, the method is high in accuracy, precision and sensitivity and includes fewer impurities; as the content detection method for the short-chain fatty acid in the human intestinal tract, themethod has good guiding significance for metabolic distribution detection of short-chain fatty acid in the human body; and a conventional GC method is improved, the peak appearance time is delayed, and the separation degree among peaks is increased, so that the sensitivity of a target drug peak is improved, and compared with an original GC method, the recovery rate is increased.
Description
technical field [0001] The invention relates to the technical field of quantitative detection, in particular to a method for quantitative detection of short-chain fatty acids in human digestive tract. Background technique [0002] Short-chain fatty acids (SCFAs) are formed primarily from colonic fermentation of indigestible carbohydrates, but small amounts of branched-chain SCFAs can also be formed from indigestible proteins. Although acetate, propionate, and butyrate are considered to be the most important SCFAs formed, feces also Isobutyric, n-valeric, isovaleric, caproic, and heptanoic acids can be found, as can lactic and succinic acids in large quantities. SCFAs in the colon are important nutrients for mucosal cells and may stimulate the proliferation of colonic bacteria and increase blood flow. Butyrate is a specific acid that may play a key role in the prevention and treatment of colon disease. Production of large amounts of SCFAs may also stimulate the body's absor...
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IPC IPC(8): G01N30/02G01N30/06G01N30/86
CPCG01N30/02G01N30/06G01N30/8634
Inventor 郝海红刘春贝王俊豪陶燕飞黄玲利王旭陈冬梅彭大鹏王玉莲潘源虎谢书宇程古月刘振利瞿伟谢长清黄啸郭金丽
Owner HUAZHONG AGRI UNIV