Unlock instant, AI-driven research and patent intelligence for your innovation.

A thermophilic bacterium producing bioemulsifier and its application

A technology of biological emulsifier and thermophilic microorganisms, which is applied in the field of thermophilic bacteria, can solve the problems of destroying soil structure and components, secondary pollution, and poor treatment effect, so as to improve oil recovery, repair environmental pollution, and treat more effect

Active Publication Date: 2022-02-18
TIANJIN INST OF IND BIOTECH CHINESE ACADEMY OF SCI
View PDF5 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Before the 1980s, only physical or chemical methods were used to control petroleum hydrocarbon-contaminated soil, but these methods would destroy the soil structure and components, cause secondary pollution, and were expensive and difficult to implement
[0004] At present, there are few studies on emulsifier-producing strains, and there are few strains that can efficiently produce bio-emulsifiers, and the emulsifiers produced by the strains are unstable and have weak tolerance to the environment. When applied, they are affected by environmental factors. Impact leads to inactivation of emulsifiers, resulting in poor treatment effects and increasing the difficulty of soil remediation

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • A thermophilic bacterium producing bioemulsifier and its application
  • A thermophilic bacterium producing bioemulsifier and its application
  • A thermophilic bacterium producing bioemulsifier and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Example 1 Bacterial Strain Geobacillus sp.L-22 Culture and Activity Analysis

[0023] 1. Strain screening

[0024] 100ml inorganic salt medium (inorganic salt medium formula: glucose 2%, sodium nitrate 0.3%, potassium dihydrogen phosphate 0.042%, dipotassium hydrogen phosphate 0.12%, ferrous sulfate 0.005%, magnesium sulfate 0.05%, calcium chloride 0.005% %, pH 7.5), add 10g Algeria oily soil, add 3% high-temperature sterilized Changqing 280-1# oilfield production fluid, place the shaker flask at 60°C, 180rpm shaker culture, pass passage once every other week. Take the third-generation flora culture solution and dilute to 10 -1 、10 -2 、10 -3 100 ul of each dilution was spread on an LB plate (LB medium formula: peptone 1%, yeast extract 0.5%, sodium chloride 1%), and placed in a 60°C incubator for cultivation. Inoculate the single colony grown on the plate into the inorganic salt crude oil medium, and culture it on a shaker at 60°C and 180rpm to observe the emulsific...

Embodiment 2

[0038] Embodiment 2: L-22 bacterial strain is to the emulsification of crude oil

[0039] The L-22 strain screened in Example 1 was cultured with inorganic salt medium for 2 days; 10% Changqing crude oil was added to the obtained bacterial liquid, and shaken at 60°C and 180rpm for 2 days, with inorganic salt medium as a control . From Figure 7 It can be seen from the figure that after adding Changqing crude oil to the L-22 bacterial solution for 2 days, it was completely dissolved in the bacterial solution, and after adding Changqing crude oil in the inorganic salt medium for 2 days, it completely floated on the upper surface of the liquid, It shows that the emulsifier produced by the L-22 strain has a strong emulsifying activity for Changqing crude oil and can dissolve the crude oil in the fermentation broth.

Embodiment 3

[0040] Application of embodiment 3 L-22 bacterial strain in processing oily soil

[0041] The soil contains a large amount of crude oil due to oil spills caused during oil exploration and transportation. In order to meet the national emission requirements, it needs to be dealt with. At present, microbial treatment is the most promising treatment method at present. The bacterial strain of the present invention has a strong emulsifying ability for crude oil, so the bacterial strain is used in the application of treating oily soil, and compared with the reported bacterial strain XS2-450 as a control strain.

[0042] The L-22 and XS2-450 strains were cultured according to the culture method in Example 1 respectively. Take 100ml of the fermentation liquid of each of the two strains, add 10g of oil sludge sample respectively, and culture it on a shaking table at 60°C and 180rpm for 24 hours, fully shake it at 60°C to emulsify the crude oil in the oil sludge, let it stand, and settl...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
surface tensionaaaaaaaaaa
Login to View More

Abstract

The invention provides a thermophilic microorganism strain and a prepared emulsifier. The thermophilic microorganism is Geobacillus sp. L-22 strain, and the strain preservation number is CGMCC NO: 20421. The emulsifier produced by the thermophilic strain L-22 of the present invention has strong tolerance to temperature, pH and salinity, and can have strong adaptability in oil-polluted environments. The strain is used in the application of treating crude oil-contaminated soil, and compared with the reported emulsifier-producing strain XS2-450, it has better treatment effect in the application of treating crude oil-contaminated soil. Therefore, the bacterial strain can be applied to petroleum industries such as microbiological enhancement of oil recovery and remediation of environmental pollution, which enriches the efficient production of bioemulsifier bacterial strains in my country.

Description

technical field [0001] The invention belongs to the technical field of microbial metabolites and biological emulsifiers, and in particular relates to a thermophilic bacterium producing biological emulsifiers and its application. Background technique [0002] Oil pollution refers to the pollution caused by crude oil and various petroleum products entering the environment during the process of mining, refining, storage, transportation, and use, among which soil pollution is particularly serious. Before the 1980s, only physical or chemical methods were used to control petroleum hydrocarbon-contaminated soil, but these methods would destroy the soil structure and components, cause secondary pollution, and were expensive and difficult to implement. The subsequent microbial remediation method has become a research hotspot in the treatment of soil oil pollution because of its low price and no secondary pollution. [0003] Bioemulsifiers play an important role in the bioremediation...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12P1/04C09K8/582C09K17/14C12R1/01
CPCC12P1/04C09K8/582C09K17/14C12R2001/01C12N1/205
Inventor 赵维马磊王兴彪侍浏洋朱婉瑜张小霞黄志勇
Owner TIANJIN INST OF IND BIOTECH CHINESE ACADEMY OF SCI