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Photoelectric energy method for promoting biological metallurgical leaching rate of microorganisms Ar-4

An optoelectronic and electronic technology, applied in the field of microorganisms, can solve the problem that the molecular biology mechanism has not been revealed yet.

Active Publication Date: 2020-12-15
INST OF MICROBIOLOGY - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the current molecular mechanism of photoelectron promotion of growth and metabolism of non-photosynthetic microorganisms has not yet been revealed.

Method used

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  • Photoelectric energy method for promoting biological metallurgical leaching rate of microorganisms Ar-4
  • Photoelectric energy method for promoting biological metallurgical leaching rate of microorganisms Ar-4
  • Photoelectric energy method for promoting biological metallurgical leaching rate of microorganisms Ar-4

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0094] Example 1. Isolation, Purification and Identification of Thermoacidophilus Metallosphaera sp.Ar-4

[0095] 1. Separation and purification identification

[0096] Metallosphaera sp.Ar-4 was isolated and purified from a sulfur-rich hot spring mud sample in Tengchong County, Yunnan Province, China on June 6, 2008. Ar-4 can utilize FeSO 4 ·7H 2 O, elemental sulfur and reduced sulfide K 2 S 4 o 6 It is an aerobic autotrophic growth for energy, has the characteristics of thermophilic, acidophilic, and metal toxicity resistance, and has autotrophic growth by oxidizing inorganic sulfur, ferrous iron or sulfide ore, heterotrophic growth by utilizing organic carbon sources, and utilization of inorganic carbon sources and The ability of organic carbon sources to grow polytrophically.

[0097] Based on the 16S rRNA gene homology of strain Ar-4, a phylogenetic tree of 16 related acidophilic archaea including Ar-4 was constructed. The species are clustered together, and have a...

Embodiment 2

[0104] Example 2, Research on the Response of Metallosphaera sp.Ar-4 to Photoelectric Energy

[0105] 1. Set up 5 groups of experiments to determine whether the strain M. cuprinaAr-4 has the ability to respond to mineral photoelectric energy

[0106] The negative electrode constant potential of -400mV was provided by the constant current potentiometer, and the photoelectric energy was simulated by the constant potential to study the response process of the strain to the photoelectron.

[0107] The experimental conditions are as follows: 1) Introducing CO 2 As the sole energy source; 2) into CO 2 , add sulfur powder as energy; 3) pass CO 2 , using Fujian Zijin Chalcopyrite as energy; 4) Natural ventilation, using Fujian Zijin Chalcopyrite as energy; 5) Natural ventilation, using Yichuan Chalcopyrite as energy. Each group of experiments was cultured without electricity as a control, and 3 groups of parallel experiments with electricity and 3 parallel experiments with no elect...

Embodiment 3

[0128] Example 3. Simulation of photoelectron catalysis for bioleaching of Aureococcus acidophilus Metallosphaera sp.Ar-4

[0129] 1. Study on M. cuprinaAr-4 bioleaching effect of simulated photoelectron catalysis

[0130] 1. Metallosphaera sp.Ar-4 seed solution

[0131] The Metallosphaera sp.Ar-4 obtained in Example 1 was cultured in a 500mL Erlenmeyer flask, and each Erlenmeyer flask was inserted with 200mL of pH 3.0 modified Allen medium, placed on a high-temperature shaker at 65°C, and cultivated at 150rpm for 7 days. Obtain Ar-4 seed solution.

[0132] 2. Inoculation and cultivation

[0133] The electrification experiment group: the Ar-4 seed liquid obtained in the above 1 is inserted into the working electrode chamber (cathode chamber) of the dual-chamber reactor that has been added with sterilized modified Allen medium according to the volume of 5% (sampling and recording the initial cell number) , and then add 2g of purple gold chalcopyrite to 100mL Ar-4 suspension,...

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Abstract

The invention discloses a photoelectric energy method for promoting the biological metallurgical leaching rate of microorganisms Ar4. The invention provides an application of photoelectron catalysis or external constant potential electrons in improving a leaching rate of target metal or target metal ions leached from sulfide metal ore by using Metallospharomyces acidophilus sp. Ar4CGMCC NO.3402 ora culture thereof or a fermentation product thereof or a suspension thereof. According to the photoelectric energy method for promoting the biological metallurgical leaching rate of the microorganisms Ar-4, under the catalytic action of simulated photoelectrons, the leaching rate of the M.cuprina Ar4 to copper ions and iron ions in the chalcopyrite is increased, and the good biological metallurgical capacity is achieved.

Description

technical field [0001] The invention belongs to the field of microorganisms, in particular to a photoelectric energy method for promoting the leaching rate of microorganism Ar-4 biometallurgy. Background technique [0002] In the practical application of biometallurgy, mesophilic (20-40°C) bacteria were mainly used at first, mostly derived from the genera Acidithiollus and Leptospirillum (Zhao et al., 2015). However, since the minerals often generate a lot of heat during the oxidation process, the temperature of the leaching tank and the leaching heap increases. In order to maintain the growth conditions of mesophilic bacteria, various cooling measures need to be taken, which greatly increases the operation of biometallurgy. cost and lead to a decline in the controllability of leaching efficiency. In addition, the generation time of mesophilic bacteria is long, the leaching efficiency is low, and the treatment efficiency of some insoluble minerals such as chalcopyrite is lo...

Claims

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Application Information

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IPC IPC(8): C22B3/18C22B15/00C12N1/20C12R1/01
CPCC12N1/20C22B3/18C22B15/0065C12N1/205C12R2001/01Y02P10/20
Inventor 姜成英刘双江宋金龙樊华周楠刘畅
Owner INST OF MICROBIOLOGY - CHINESE ACAD OF SCI
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