Photoelectric energy method for promoting biological metallurgical leaching rate of microorganisms Ar-4

An optoelectronic and electronic technology, applied in the field of microorganisms, can solve the problem that the molecular biology mechanism has not been revealed yet.

Active Publication Date: 2020-12-15
INST OF MICROBIOLOGY - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the current molecular mechanism of photoelectron promotion of growth

Method used

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  • Photoelectric energy method for promoting biological metallurgical leaching rate of microorganisms Ar-4
  • Photoelectric energy method for promoting biological metallurgical leaching rate of microorganisms Ar-4
  • Photoelectric energy method for promoting biological metallurgical leaching rate of microorganisms Ar-4

Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0094] 1, acidophilic thermophilic bacteria Metallosphaera golden Purification Example identified sp.Ar-4

[0095] I. Identification of Purification

[0096] Eosinophilic thermophilic golden cocci Metallosphaera sp.Ar-4 2008 June 6 separated from the hot springs rich in sulfur mud-like Tengchong County, Yunnan Province of China purified get. Ar-4 available FeSO 4 · 7h 2 O, elemental sulfur and sulfides reduced K 2 S 4 O 6 Energy aerobic autotrophic growth, with thermophilic, acidophilic, anti-metal toxic properties, but also oxidation of inorganic sulfur, ferrous sulfide ore or autotrophic growth, using an organic carbon source heterotrophically and the use of inorganic carbon and polyculture ability to grow organic carbon.

[0097] Strain Gene Ar-4 16S rRNA homology based phylogenetic tree comprises Ar-4, including the 16 associated acidophilic archaea, the phylogenetic analysis showed that the three strains Ar-4 Metallosphaera genera species together into one, and has a high app...

Example Embodiment

[0104] 2, Response golden acidophilic thermophilic bacteria Metallosphaera sp.Ar-4 Example photoelectric energy

[0105] A provided set of experiments 5 M.cuprinaAr-4 strain determined whether they have the ability to respond photoelectric mineral

[0106] A cathode electrode provided -400mV galvanostatic potentiostatic potentiostat, using a potentiostatic simulated photovoltaic energy, research strains in response to the photoelectrons process.

[0107] The experimental conditions were as follows: 1) into CO 2 As the only energy; 2) into CO 2 , Was added as sulfur powder energy; 3) into CO 2 , Made with purple Fujian chalcopyrite energy; 4) natural ventilation, by doing Fujian purple chalcopyrite energy; 5) of natural ventilation, by Yi Chuan do chalcopyrite energy. Each set of experiments are not energized culture as control, and three sets of power-culture experiments and 3 groups of parallel cultures parallel experiments not energized.

[0108] The electrochemical apparatus us...

Example Embodiment

[0128] 3 embodiment, simulation photoelectron catalytic acidophilic thermophilic bacteria golden Metallosphaera sp.Ar-4 bioleaching Example

[0129] A, Effect of analog optoelectronic catalytic bioleaching M.cuprinaAr-4

[0130] 1, Metallosphaera sp.Ar-4 seed liquid

[0131] The embodiment of Example 1 to give Metallosphaera sp.Ar-4 in 500mL conical flasks, Erlenmeyer flasks each medium access improved Allen 200mL pH 3.0, and placed in a shaker at temperature 65 deg.] C, 150 rpm for 7 days Ar-4 to give seed liquid.

[0132] 2, the seed culture

[0133] Energizing experimental groups: after the above 1 Ar-4 seed was obtained according to a volume of 5% has been added to the access medium sterilized Allen improved two-compartment reactor chamber a working electrode (cathode chamber) (Sample Record initial cell number) and then adding 2g of chalcopyrite purple suspension 100mLAr-4, the culture was adjusted to a pH 3.0,65 initial energization of the culture (leaching reactor) up to 14...

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Abstract

The invention discloses a photoelectric energy method for promoting the biological metallurgical leaching rate of microorganisms Ar4. The invention provides an application of photoelectron catalysis or external constant potential electrons in improving a leaching rate of target metal or target metal ions leached from sulfide metal ore by using Metallospharomyces acidophilus sp. Ar4CGMCC NO.3402 ora culture thereof or a fermentation product thereof or a suspension thereof. According to the photoelectric energy method for promoting the biological metallurgical leaching rate of the microorganisms Ar-4, under the catalytic action of simulated photoelectrons, the leaching rate of the M.cuprina Ar4 to copper ions and iron ions in the chalcopyrite is increased, and the good biological metallurgical capacity is achieved.

Description

technical field [0001] The invention belongs to the field of microorganisms, in particular to a photoelectric energy method for promoting the leaching rate of microorganism Ar-4 biometallurgy. Background technique [0002] In the practical application of biometallurgy, mesophilic (20-40°C) bacteria were mainly used at first, mostly derived from the genera Acidithiollus and Leptospirillum (Zhao et al., 2015). However, since the minerals often generate a lot of heat during the oxidation process, the temperature of the leaching tank and the leaching heap increases. In order to maintain the growth conditions of mesophilic bacteria, various cooling measures need to be taken, which greatly increases the operation of biometallurgy. cost and lead to a decline in the controllability of leaching efficiency. In addition, the generation time of mesophilic bacteria is long, the leaching efficiency is low, and the treatment efficiency of some insoluble minerals such as chalcopyrite is lo...

Claims

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Application Information

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IPC IPC(8): C22B3/18C22B15/00C12N1/20C12R1/01
CPCC12N1/20C22B3/18C22B15/0065C12N1/205C12R2001/01Y02P10/20
Inventor 姜成英刘双江宋金龙樊华周楠刘畅
Owner INST OF MICROBIOLOGY - CHINESE ACAD OF SCI
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