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Application of youngia japonica in acaricidal and insecticidal aspects as well as biological control medicament and preparation method thereof

A kind of red fruit yellow quail, aspect technology, applied in the direction of biocide, botanical equipment and method, chemical used for biological control, etc., can solve the problems such as the application of red fruit yellow quail to kill mites and insects that have not been reported

Active Publication Date: 2020-12-18
CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The acaricide and insecticide application of red fruit yellow quail has not been reported in this field

Method used

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  • Application of youngia japonica in acaricidal and insecticidal aspects as well as biological control medicament and preparation method thereof
  • Application of youngia japonica in acaricidal and insecticidal aspects as well as biological control medicament and preparation method thereof
  • Application of youngia japonica in acaricidal and insecticidal aspects as well as biological control medicament and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0051] The embodiment of this group provides a preparation method of biological control agent. All the examples in this group have the following common features: the preparation method includes: boiling or distilling, condensing, and extracting the aerial parts of red-fruited yellow quail.

[0052] In a specific embodiment, the distillation refers to steam distillation for 4-8 hours, 6 hours to fully extract the volatile components in the plant material.

[0053] The poaching refers to boiling with water for 4-8 hours, preferably 6 hours, so that the essential oil components can be boiled out from the fresh plants as completely as possible.

[0054] Preferably, the extraction refers to extraction with n-hexane;

[0055] More specifically, a 2L separatory funnel was used, n-hexane:distillate=1:1, and the n-hexane layer in which the essential oil had been dissolved was retained after layering.

[0056] Preferably, the temperature of the water bath for the extraction is 38-45°C...

experiment example 1

[0061] Experimental example 1, the preparation of red fruit yellow quail essential oil

[0062] Collect the above-ground part of the red-fruited yellow quail and treat it with water for 6 hours. The obtained oil-water mixture is condensed and recovered and then extracted with n-hexane. After drying with anhydrous sodium sulfate, use a rotary evaporator to remove the n-hexane, and the obtained ingredient is red-fruited yellow quail essential oils. Wherein, the temperature of the water bath is 40°C.

[0063] The yield of the essential oil of red fruit yellow quail is 0.30% (w / w), that is, 100 g of fresh red fruit yellow quail plants can extract 0.3 g of red fruit yellow quail essential oil.

experiment example 2

[0064] Experimental example 2. Identification of essential oil components of red fruit and yellow quail

[0065] GC-MS detection method was used to identify the essential oil components of red fruit and yellow quail:

[0066] Detection instruments: gas chromatograph (Agilent6890N) and mass spectrometer (Agilent5973N). .

[0067] Detection conditions: HP-5MS 30mm×0.25mm×0.25μm quartz capillary column, the initial column temperature is 60°C, keep it for 1min, at the rate of 12°C / min, program the temperature to 180°C and keep it for 0.5min, then at the rate of 25°C / min The temperature was programmed to 280°C for 18 minutes, the vaporizer temperature was 280°C, the carrier gas was helium, the column flow rate was 1.0mL / min, and the split ratio was 1:10.

[0068] The mass spectrometer ionization mode is EI, the electron bombardment energy is 70eV, the scanning mass range is 20-550m / z, and the scanning time is 2s.

[0069] Take 1 μL (100 times acetone dilution) of red fruit yello...

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Abstract

The invention discloses an application of youngia japonica in acaricidal and insecticidal aspects as well as a biological control medicament and a preparation method thereof, and belongs to the fieldof biological control. The invention relates to the application of youngia japonica in acaricidal and insecticidal aspects, and the biological control medicament based on youngia japonica essential oil. The biological control agent can efficiently kill pest mites and storage pests, and is non-toxic, harmless, safe, reliable and low in cost.

Description

technical field [0001] The invention belongs to the field of biological control, and in particular relates to the use of red-fruited yellow quail in killing mites and insects, as well as its biological control agent and preparation method. Background technique [0002] Two-spotted spider mite (Tetranychusurticae) is a kind of organism that seriously harms crops. Because of its wide host range, small size, fast reproduction, concealment and strong drug resistance, and two-spotted spider mite mainly feeds on the back of crop leaves, it is A class of pests that are difficult to control. At present, the main measure to prevent and control Tetranychus urticae is the application of acaricides. Under the pressure of long-term selection of medicaments, the resistance of Tetranychus urticae is gradually increasing; at the same time, the use of chemical agents not only prevents mites, but also affects African species. The target organisms also have a killing effect, disrupting the ec...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N65/12A01P7/00A01P7/02
CPCA01N65/00A01N65/12
Inventor 刘志龙赵燚刘奇志
Owner CHINA AGRI UNIV
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