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Culture medium for large-scale culture of chlorella and culture method of culture medium

A technology of large-scale cultivation and cultivation method, which is applied in the field of culture medium for large-scale cultivation of chlorella, can solve the problems of being unsuitable for large-scale cultivation, low cultivation efficiency, and affecting the growth efficiency of chlorella, and achieves the goal of production process and cultivation The method is simple and convenient, and the effect of broad application prospects

Inactive Publication Date: 2020-12-22
ANHUI TECH BANK BIO TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Culture substrates and methods affect the growth efficiency of Chlorella, and the current Chlorella culture medium has the disadvantages of not being suitable for large-scale cultivation and low cultivation efficiency.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Weigh each component and dissolve in distilled water to prepare 700L culture medium: CH 4 N 2 O 520mg / L, K 2 HPO 4 60mg / L, MgSO 4 ·7H 2 O 170mg / L, CaCl 2 2H 2 O 22mg / L, citric acid 6mg / L, ferric citrate 6.0mg / L, EDTANA 2 1.2mg / L, sea green 201mg / L, H 3 BO 4 2.7mg / L, MnCl 2 4H 2 O 1.9mg / L, ZnSO 4 ·7H 2 O 0.20mg / L, Na 2 MoO 4 2H 2 O 0.40mg / L, CuSO 4 ·5H 2 O 0.6mg / L, C 4 h 6 CoO 4 4H 2 O 0.05mg / L. The pH value is 7.0. Inoculate the cultivated Chlorella into a translucent 1000L culture bucket containing 700L culture solution, so that the initial cell concentration is 5×10 6 cell / mL, at a temperature of 20°C and a light intensity of 3800 lx, aerate the algae solution for 80 minutes at 9:00, 12:00, and 3:00 every day. During the cultivation period, take a small amount of culture solution from the culture bucket every day, and use a hemocytometer to count to determine the growth stage of the algae cells. When the algae cell density reaches 5×10 7 ~1×...

Embodiment 2

[0022] Weigh each component and dissolve in distilled water to prepare 700L culture medium: CH 4 N 2 O 530mg / L, K 2 HPO 4 58mg / L, MgSO 4 ·7H 2 O 165mg / L, CaCl 2 2H 2 O 22.5mg / L, citric acid 5.5mg / L, ferric citrate 5.8mg / L, EDTANA 2 1mg / L, sea green pigment 200mg / L, H 3 BO 4 2.65mg / L, MnCl 2 4H 2 O 1.85mg / L, ZnSO 4 ·7H 2 O 0.225mg / L, Na 2 MoO 4 2H 2O 0.38mg / L, CuSO 4 ·5H 2 O 0.32mg / L, C 4 h 6 CoO 4 4H 2 O 0.045mg / L. The pH value is 7.2. Inoculate the cultivated Chlorella into a translucent 1000L culture bucket containing 700L culture solution, so that the initial cell concentration is 3×10 6 cell / mL, at a temperature of 25°C and a light intensity of 3800 lx, the algae solution was aerated for 60 minutes at 9 o'clock, 12 o'clock, and 3 o'clock every day. During the cultivation period, take a small amount of culture solution from the culture bucket every day, and use a hemocytometer to count to determine the growth stage of the algae cells. When the alga...

Embodiment 3

[0025] Weigh each component and dissolve in distilled water to prepare 700L culture medium: CH 4 N 2 O 535mg / L, K 2 HPO 4 60mg / L, MgSO 4 ·7H 2 O 168mg / L, CaCl 2 2H 2 O 22mg / L, citric acid 6mg / L, ferric citrate 6.0mg / L, EDTANA 2 1.2mg / L, sea green pigment 198mg / L, H 3 BO 4 2.6mg / L, MnCl 2 4H 2 O 1.8mg / L, ZnSO 4 ·7H 2 O 0.20mg / L, Na 2 MoO 4 2H 2 O 0.36mg / L, CuSO 4 ·5H 2 O 0.4mg / L, C 4 h 6 CoO 4 4H 2 O 0.04mg / L. The pH value is 7.3. Inoculate the cultured Chlorella into a translucent 1000L culture bucket containing 700L culture solution, so that the initial cell concentration is 4×10 6 cell / mL, at a temperature of 30°C and a light intensity of 3800 lx, aerate the algae solution for 100 minutes at 9 o'clock, 12 o'clock, and 3 o'clock every day. During the cultivation period, take a small amount of culture solution from the culture bucket every day, and use a hemocytometer to count to determine the growth stage of the algae cells. When the algae cell densi...

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Abstract

The invention discloses a culture medium for large-scale culture of chlorella and a culture method of the culture medium. The culture medium comprises the following components: CH4N2O, K2HPO4, MgSO4.7H2O, CaCl2.2H2O, citric acid, ferric citrate, EDTANA2, glauconite, H3BO4, MnCl2.4H2O, ZnSO4.7H2O, Na2MoO4.2H2O, CuSO4.5H2O and C4H6CoO4.4H2O. The culture method comprises the following steps: inoculating the chlorella into the culture medium, wherein the initial inoculation amount is 3*10<6>-5*10<6> cell / mL; inflating the chlorella liquid for 60-120 minutes at 9 o'clock, 12 o'clock and 3 o'clock every day in ordinary sunny days at the temperature of 20-35 DEG C to prevent the chlorella cells from sinking to the bottom; and when the chlorella cell density reaches 5*10<7>-1*10<8> cell / mL, finishing the culture. The culture medium is different from a common chlorella culture medium, and is low in cost, and the growth and reproduction speed of the chlorella can be remarkably increased, so thatthe chlorella cell density is remarkably increased within a short time, and the culture period is short. In combination with the culture method disclosed by the invention, a large-scale high-densityculture mode of the chlorella can be quickly realized.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a medium for large-scale cultivation of chlorella and a cultivation method thereof. Background technique [0002] Chlorella is a spherical single-celled green alga with a diameter of 3-8 μm. It grows and reproduces by photosynthesis. It is the first artificially cultivated microalgae. It is rich in nutrients such as amino acids, proteins, polysaccharides, and unsaturated fatty acids. , has high healthcare value. In aquaculture, chlorella promotes the growth and reproduction of zooplankton such as rotifers, copepods, cladocerans and artemia, and can also be used as a high-quality opening bait for many fish, shrimp, and shellfish. Provide adequate nutrients. In addition, chlorella can consume some organic fertilizers such as nitrogen and phosphorus in the water body during the growth process, so as to achieve the purpose of purifying and regulating water quality. It is an important m...

Claims

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Application Information

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IPC IPC(8): C12N1/12C12R1/89
CPCC12N1/12
Inventor 胡传炯李姗周小波
Owner ANHUI TECH BANK BIO TECH
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