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A method for the determination of selenium speciation in selenium-enriched plant dry powder

A technology for selenium-enriched plants and dry powder, which is applied in the field of analysis and testing, can solve the problems of high cost of protease, selenium form conversion, long reaction time, etc., and achieves the effects of high extraction efficiency, short time and simple operation.

Active Publication Date: 2022-04-01
JIANGNAN UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The existing selenium form separation method has a long reaction time, which easily leads to the conversion of selenium form; and the selected protease is expensive, and its promotion in application is limited

Method used

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  • A method for the determination of selenium speciation in selenium-enriched plant dry powder
  • A method for the determination of selenium speciation in selenium-enriched plant dry powder
  • A method for the determination of selenium speciation in selenium-enriched plant dry powder

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] (1) Dissolve 0.1 g of dry powder of rice chestnuts in 5 mL of deionized water, control the temperature at 40°C, and ultrasonicate at 40KHz for 15 minutes. After the ultrasonication is completed, centrifuge the obtained mixture at 4000 rpm for 15 minutes and take the supernatant to obtain supernatant 1;

[0036] (2) Dissolve 1 mg of sodium dodecyl sulfate (SDS) in 7 mL of Tris-HCL with a pH of 7.5, dissolve the precipitate after centrifugation in all steps (1), and add 10 mg of flavor protease (Flavourzyme500MG, Novozymes Biotechnology Co., Ltd.) was stirred for 4 hours, and then 10 mg of protease E (Pronase E, Type XIV, ≥3.5 units / mg, Sigma-aldrich Company) was stirred for 4 hours, and the temperature of the enzyme was 40 °C. After solution, centrifuge at 4000rpm for 15min to take the supernatant to obtain supernatant 2;

[0037] (3) Take the centrifuged precipitate in step (2) and repeat the operation in step (2) for enzymolysis. After enzymolysis, centrifuge at 4000rp...

Embodiment 2

[0048] (1) Dissolve 0.1g of dry cabbage powder in 5mL of deionized water, control the temperature at 40°C, and ultrasonicate at 40KHz for 25min. After the ultrasonication is completed, centrifuge the obtained mixture at 4000rpm for 15min and take the supernatant to obtain supernatant 1;

[0049] (2) Dissolve 1 mg of SDS in 7 mL of Tris-HCL with pH 7.5, dissolve the precipitate after centrifugation in step (1), first add 10 mg of flavor protease and stir for 4 hours, then add 10 mg of protease E and stir Enzyme hydrolysis for 4 hours, the temperature of enzymolysis is 40°C, after enzymolysis, centrifuge at 4000 rpm for 15 minutes to take the supernatant, and obtain supernatant 2;

[0050] (3) Take the centrifuged precipitate in step (2) and repeat the operation in step (2) for enzymolysis. After enzymolysis, centrifuge at 4000rpm for 15min to take the supernatant to obtain supernatant 3;

[0051] (4) Collect the supernatant obtained in each step and filter it through a 0.45 μm ...

Embodiment 3

[0053] (1) Dissolve 0.1g of broccoli dry powder in 7mL of deionized water, control the temperature at 40°C, and ultrasonicate at 40KHz for 15min. After the ultrasonication is completed, centrifuge the obtained mixture at 4000rpm for 15min and take the supernatant to obtain supernatant 1;

[0054] (2) Dissolve 1 mg of SDS in 7 mL of Tris-HCL with pH 7.5, dissolve the precipitate after centrifugation in step (1), first add 10 mg of flavor protease to it and stir for 5 hours, then add 10 mg of protease E and stir Enzymolysis was performed for 5 hours at a temperature of 40°C. After enzymolysis, centrifuge at 4000 rpm for 15 minutes to obtain supernatant 2;

[0055] (3) Take the centrifuged precipitate in step (2) and repeat the operation in step (2) for enzymolysis. After enzymolysis, centrifuge at 4000rpm for 15min to take the supernatant to obtain supernatant 3;

[0056] (4) Collect the supernatant obtained in each step and filter it through a 0.45 μm nylon filter head. The con...

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Abstract

The invention discloses a method for determining the form of selenium in selenium-enriched plant dry powder, belonging to the field of analysis and testing. Ultrasound-assisted extraction of free selenium-containing substances in selenium-enriched plant dry powder, after centrifugation, an extraction solution containing sodium dodecyl sulfate (SDS) was added to the sediment, and a double-enzyme method was used to extract bound selenium-containing substances twice . Collect the supernatant obtained in each step to pass through the membrane, inject the concentrated solution into the GPC system for separation, collect small molecular fractions, concentrate and use liquid chromatography-atomic fluorescence spectrometer (HPLC-AFS) or high performance liquid chromatography-inductor Coupled plasma mass spectrometry (HPLC‑ICP‑MS) was used to determine the content of each selenium species. The method has the advantages of short extraction time, high extraction efficiency, low enzyme cost and stable selenium form during the process.

Description

technical field [0001] The invention relates to a method for determining the form of selenium in selenium-enriched plant dry powder, belonging to the field of analysis and testing. Background technique [0002] Selenium is an essential trace element for the human body and is listed as one of the 15 nutrients. Selenium has anti-oxidation, anti-tumor, anti-inflammation, anti-aging and other effects. Many countries and regions are in a state of dietary selenium deficiency, and 70% of my country is in a selenium deficiency zone. It is reported that the daily selenium intake of many adults is lower than the recommended dose of the Chinese Nutrition Society. When selenium is deficient in the human body, it will cause many diseases, so daily selenium supplementation is very necessary. Now the development of safe and effective selenium supplement products has become the focus of selenium-enriched health care products in my country. The utilization value of many selenium supplement...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/06G01N30/60G01N30/88
CPCG01N30/06G01N30/60G01N30/88
Inventor 朱松林樾丛欣于添李玥
Owner JIANGNAN UNIV