Application of Picrite Compounds in Controlling Plant Fungal Diseases
A technology of plant fungal diseases and bitters, which is applied in the field of pesticides, can solve problems such as pollution, residual environment, and increased drug resistance of pathogenic bacteria, and achieve the effect of convenient use and good application prospects
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Embodiment 1
[0048] Example 1: Extraction, Separation and Structural Identification of Bitterpicrin Compounds from Tongkat Ali, a Bitterwoodaceae Plant
[0049] Tongkat Ali root 10kg, pulverized into coarse powder, extracted by cold soaking with 95% ethanol, combined extracts, concentrated under reduced pressure to obtain 270g of total extract. Suspend the total extract with water, extract sequentially with petroleum ether, ethyl acetate, and n-butanol, and recover the solvent under reduced pressure respectively to obtain petroleum ether fraction (40g), ethyl acetate fraction (99g), and n-butanol fraction (85g) and water fractions (26g).
[0050] The petroleum ether part was subjected to silica gel column chromatography, chloroform-methanol system gradient elution (100:0→0:100, v / v), combined by TLC analysis, and 7 main fractions (Fr.a-Fr.g) were obtained .
[0051] Fr.e was subjected to ODS column chromatography, methanol-water gradient elution (20:80→100:0, v / v), combined by TLC analys...
Embodiment 2
[0074] Example 2: Inhibitory effect of picrite compounds on phytopathogenic fungi
[0075] (1) Test drug
[0076] Picropicrin compounds (compounds 1-9), prochloraz (positive control compound, commercial pesticide).
[0077] (2) The tested fungal strains
[0078] There are 6 fungal strains tested, which are Fusarium spp. causing banana wilt, Glionesporium anthracnose causing papaya anthracnose, Thielavia parvum causing guava leaf blight, and Fusarium solani causing dry rot of potato. fungus, apple rot for apple rot and Botrytis cinerea for tomato early blight.
[0079] (3) Determination of anti-plant fungal activity
[0080]Preparation of the test bacteria plate: under sterile conditions, inoculate the pure strains of the test bacteria on the sterile PDA medium plate with dense wave line drawing method, and cultivate it at 27°C for about one week. The plate is covered with a lawn. use.
[0081] Preparation of medium and its sterilization: the medium is PDA solid medium, an...
Embodiment 3
[0090] Example 3: Compound 8 (eurycomanone) can be sucked into the inside of the banana root through the root from the growth environment of the banana, and transported upwards to inhibit the banana wilt
[0091] Dissolve eurycomanone with methanol to make a 10000μg / mL mother solution, and then add 0.5mM CaCl 2 Dilute the stock solution with aqueous solution to 100 μg / mL culture solution. Banana seedlings (6-leaf stage, not previously treated with eurycomanone) that had been cultivated in advance were placed in 0.5mM CaCl 2 After pre-cultivation in aqueous solution for 2 hours, transfer it to the prepared eurycomanone culture solution. After 24 hours, take 0.5 grams of banana stems and leaves, mash them and dissolve them in 2 mL of methanol. The extract can be detected by high performance liquid chromatography. Contains eurycomanone in banana, after calculation, the content of eurycomanone in banana stem and top leaf (fresh leaf) is 67μg / g and 53μg / g respectively. The leaves...
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