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A method for extracting and separating zeaxanthin and palmitate pigment monomers in wolfberry fruit

A technology of zeaxanthin and palmitate, which is applied in the direction of organic chemistry, can solve the problems of small number of compounds, separation methods that cannot be simple, fast, efficient, large-scale preparation, and low purity, and achieve improved yield, The separation method is simple and efficient, and the effect of increasing the injection volume and output

Active Publication Date: 2021-07-13
LANZHOU INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Most of the literature related to the extraction and separation of carotenoids in wolfberry concentrates on the extraction of wolfberry pigment mixture and the improvement of the total pigment content. There are few reports on the separation and preparation of wolfberry pigment monomer compounds, and the number of compounds obtained is small and the purity is high. Low, the separation method used cannot meet the needs of simple, fast, efficient, and large-scale preparation

Method used

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  • A method for extracting and separating zeaxanthin and palmitate pigment monomers in wolfberry fruit
  • A method for extracting and separating zeaxanthin and palmitate pigment monomers in wolfberry fruit

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] (1) Extraction of the crude extract of Lycium barbarum pigment: crush the dried Lycium barbarum fruit and add it to n-hexane, and reflux extraction in a water bath at 62°C for 60 minutes to obtain the extract; Dry under reduced pressure to obtain crude extract of wolfberry pigment;

[0020] (2) Preparation of high-speed countercurrent chromatography solvent system: Mix n-hexane, dichloromethane, and acetonitrile at a volume ratio of 10:3.5:6.5 to obtain a solvent system;

[0021] (3) Preparation of sample solution: prepare the crude extract of wolfberry pigment obtained in step (1) with the solvent system of step (2) to prepare a sample solution with a concentration of 90 mg / mL;

[0022] (4) High-speed countercurrent chromatographic separation of the elution-extrusion of the second-order overlapping repeated injection: the solvent system obtained in step (2) is shaken in a separatory funnel and then separated into layers to obtain the upper and lower phases of the solve...

Embodiment 2

[0028] (1) Extraction of Lycium barbarum pigment crude extract: Same as Example 1;

[0029] (2) Preparation of high-speed countercurrent chromatography solvent system: Mix n-hexane, dichloromethane, and acetonitrile at a volume ratio of 10:3:7 to obtain a solvent system;

[0030] (3) Preparation of sample solution: prepare the crude extract of wolfberry pigment obtained in step (1) with the solvent system of step (2) to prepare a sample solution with a concentration of 90 mg / mL;

[0031] (4) High-speed countercurrent chromatographic separation of the elution-extrusion of the second-order overlapping repeated injection: the solvent system obtained in step (2) is shaken in a separatory funnel and then separated into layers to obtain the upper and lower phases of the solvent system ; Respectively, the upper phase and the lower phase are used as the stationary phase of the high-speed counter-current chromatographic separation and the mobile phase of the high-speed counter-current ...

Embodiment 3

[0036] (1) Extraction of the crude extract of wolfberry pigment: the extraction process is the same as in Example 1;

[0037] (2) Preparation of high-speed countercurrent chromatography solvent system: Mix n-hexane, dichloromethane, and acetonitrile at a volume ratio of 10:4:6.5 to obtain a solvent system;

[0038] (3) Preparation of sample solution: prepare the crude extract of wolfberry pigment obtained in step (1) with the solvent system of step (2) to prepare a sample solution with a concentration of 90 mg / mL;

[0039] (4) High-speed countercurrent chromatographic separation of the elution-extrusion of the second-order overlapping repeated injection: the solvent system obtained in step (2) is shaken in a separatory funnel and then separated into layers to obtain the upper and lower phases of the solvent system ; Then the upper phase and the lower phase are used as the stationary phase and the mobile phase of the high-speed counter-current chromatographic separation respect...

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Abstract

The invention discloses a method for extracting and separating zeaxanthin and its palmitate pigment monomer in wolfberry fruit. The mixed solvent of alkane, dichloromethane and acetonitrile is used as the solvent system for countercurrent chromatographic separation, and the upper phase and the lower phase are obtained after standing in a separatory funnel; then the upper phase and the lower phase are used as the stationary phase and the mobile phase respectively, and passed Three pigment monomers, zeaxanthin, zeaxanthin monopalmitate and zeaxanthin dipalmitate, were separated by the method of elution-extrusion high-speed countercurrent chromatography based on the second-order overlapping repeated injection, and the purity reached more than 85%. , the yields of the three monomeric compounds were above 3.5%, 5.0%, and 19.9%, respectively. The preparation method of the invention has the characteristics of large sample amount, less solvent consumption, large preparation amount, etc., and can realize the macro-preparation of zeaxanthin and its ester pigment monomers in wolfberry.

Description

technical field [0001] The invention relates to a method for extracting carotenoids in wolfberry fruit, in particular to a method for extracting and separating zeaxanthin and palmitate pigment monomer three types of monomer compounds in wolfberry fruit, belonging to the field of natural product separation methods. Background technique [0002] Lycium barbarum as wolfberry ( Lycium barbarum.L ) are widely used as functional foods and dietary supplements due to their rich content of active ingredients. In addition to being a traditional Chinese medicine, modern pharmacological research has once again confirmed that it has various pharmacological activities beneficial to human health, such as antioxidant activity, anti-aging, immune regulation, anti-tumor, hypoglycemic, liver protection and neuroprotection function and so on. Therefore, wolfberry is also known as the plant fruit of "the same medicine and food". It is precisely because of the increasing health care function o...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07C403/24
CPCC07C403/24C07C2601/16
Inventor 邸多隆巩媛黄新异
Owner LANZHOU INST OF CHEM PHYSICS CHINESE ACAD OF SCI