Bovine pasteurella multocida inactivated vaccine and preparation method thereof
A technology of Pasteurella and inactivated vaccines, which is applied in the field of inactivated vaccines against Pasteurella multocida and its preparation, which can solve the problems of low number of Pasteurella cultured viable bacteria, poor product potency, and toxic and side effects and other problems, to achieve the effect of increasing the number of viable bacteria, improving quality, and reducing side effects
Pending Publication Date: 2021-01-01
CHONGQING AULEON BIOLOGICALS
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Problems solved by technology
However, under the existing production process conditions, the number of viable Pasteurella multocida cultured bacteria is low, and the cultured bacteria solution is not purified and directly mixed with vaccines, the product potency is not good, and in c
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Embodiment 1
[0031] The preparation method of bovine pasteurellosis multocida inactivated vaccine in the present embodiment may further comprise the steps:
[0032] 1) Primary seed preparation: Inoculate 1% of each freeze-dried strain into Martin broth medium containing 0.1% lysed whole blood and 4% healthy horse serum, and culture on a shaker at 150rpm at 37°C After 12 hours, the culture was streaked on a modified Martin agar plate containing 0.5% lysed whole blood and 4% healthy horse serum, cultivated for 12 hours at 37°C, and more than 5 smooth typical colonies were selected and inoculated on A number of blood slant were cultivated for 24 hours at 37°C to obtain first-grade seeds;
[0033] 2) Secondary seed preparation: inoculate the primary seeds of each strain in the Martin Broth medium containing 0.1% lysed whole blood and 4% healthy horse serum according to the inoculum size of 1%, and culture them on a shaking table at 37°C for 12 Hours, microscopic examination and pure inspectio...
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The invention discloses a bovine pasteurella multocida inactivated vaccine and a preparation method thereof, and belongs to the technical field of veterinary biological products. The vaccine comprisesantigens and adjuvants, wherein the antigens are a bovine pasteurella multocida capsule type B C45-2 strain and a C47-2 strain. The preparation method comprises the following steps: performing inoculated culture on strains, performing shake culture, streaking a culture on an agar plate for culture, selecting bacterial colonies, inoculating a fresh blood slant with the selected bacterial colonies,performing culturing to obtain primary seeds, inoculating a culture medium with the primary seeds, and performing shake culture to obtain secondary seeds; mixing the secondary seeds, and performing inoculated culture to obtain zymophyte liquid, wherein the culture process is optimized, so that the antigen quantity is multiplied; and performing centrifuging to remove endotoxin, transferring bacterial sludge into normal saline, adding formaldehyde, performing inactivating, adding aluminum hydroxide glue, thiomersalate and phenol, performing full oscillating, and performing sub-packaging. The dosage of the bovine pasteurella multocida inactivated vaccine for immunizing a calf is 2-3mL and is less than the existing injection dosage, the side effect is greatly reduced, and the bovine pasteurella multocida inactivated vaccine has popularization and application values in the fields of bovine pasteurella multocida inactivated vaccines and even all bacterial culture processes.
Description
technical field [0001] The invention belongs to the technical field of veterinary biological products, and in particular relates to an inactivated vaccine for bovine pasteurellosis multocida and a preparation method thereof. Background technique [0002] Bovine Pasteurella multocida is an infectious disease caused by Pasteurella multocida infection in cattle. At present, there are mainly two types of Pasteurella multocida prevailing in my country, one is bovine fibrinous suppurative pneumonia caused by Pasteurella multocida capsule type A bacteria, and the other is caused by Pasteurella multocida capsule type A bacteria, and the other is caused by Pasteurella multocida capsule type A bacteria, Hemorrhagic septicemia in cattle caused by Pasteurella spp. capsulatus type B. Among them, bovine hemorrhagic septicemia is an infectious disease characterized by high fever, pneumonia, acute gastroenteritis and extensive hemorrhage of internal organs, with a high fatality rate. The d...
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Login to view more IPC IPC(8): A61K39/102A61P31/04C12N1/20C12R1/01
CPCA61K39/102A61K2039/521A61K2039/552A61K2039/55505A61P31/04C12N1/20
Inventor 王崇俊刘昕唐茂森黎国华唐杰杨梅
Owner CHONGQING AULEON BIOLOGICALS
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