Preparation method of high-efficiency composite probiotic Bestroin for poultry intestinal tracts
A technology of compound probiotics and Baijialining, which is applied in the biological field, can solve problems such as chemical and respiratory tract infections, poor feeding management, and low product quality, and achieve the effects of improving health, inhibiting intestinal diseases, and improving quality
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[0019] Put 5 parts of Lactobacillus acidophilus, 5 parts of Bacillus subtilis, 5 parts of Enterococcus and 5 parts of Bacillus Velez into the culture medium at a temperature of 37°C and culture for 24 hours to obtain Lactobacillus acidophilus Bacillus subtilis solution, Enterococcus solution and Bacillus Veles solution; secondly, weigh 20 grams of culture medium, put it into 800 ml of water, mix well, divide into four parts, sterilize for 20 minutes, and then Add 3ml each of the obtained Lactobacillus acidophilus bacterial liquid, Bacillus subtilis bacterial liquid, Enterococcus bacterial liquid and Bacillus beilesi bacterial liquid to four parts of LB liquid respectively. 150r / min, expanded culture and adjustment, the concentration of Lactobacillus acidophilus bacteria solution was 0.3×88~1.0×108CFU / ml, the concentration of Bacillus subtilis bacteria solution was 3.5-6.1×1012CFU / ml, and the concentration of Enterococcus bacteria solution The concentration of Bacillus subtilis...
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