Preparation method of high-efficiency composite probiotic Bestroin for poultry intestinal tracts

A technology of compound probiotics and Baijialining, which is applied in the biological field, can solve problems such as chemical and respiratory tract infections, poor feeding management, and low product quality, and achieve the effects of improving health, inhibiting intestinal diseases, and improving quality

Inactive Publication Date: 2021-01-12
陆良佰佳益康生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] With the increasing intensification of the current farming industry, the development trend of poultry diseases is becoming more and more complex and changeable; poultry colibacillosis is a disease caused by pathogenic Escherichia coli, which is found in nature Widely distributed, such as air, dust, litter, feed, drinking water, poultry body surface, hatchery, incubator and other appliances in poultry houses, there are many poultry diseases, which will lead to low product quality, and the remaining poultry will cause food safety problems Threats to human health, and poultry feces contamination of eggs is the main source of infection in poultry, because the contaminated egg Escherichia coli can invade through the eggshell and shell membrane to cause poultry embryo infection, and can also be infected through the digestive tract and respiratory tract. Poor feeding and management, poor sanitation, malnutrition and other diseases can induce this disease. Because poultry colibacillosis is easy to be co-infected with mycoplasma disease, it is often secondary to other infectious diseases such as Newcastle disease, avian flu infection, infectious disease, etc. Bronchitis, pasteurellosis, etc. are very difficult to treat and have become important infectious diseases that endanger the breeding industry, bringing huge economic losses

Method used

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Examples

Experimental program
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Effect test

Embodiment 1

[0019] Put 5 parts of Lactobacillus acidophilus, 5 parts of Bacillus subtilis, 5 parts of Enterococcus and 5 parts of Bacillus Velez into the culture medium at a temperature of 37°C and culture for 24 hours to obtain Lactobacillus acidophilus Bacillus subtilis solution, Enterococcus solution and Bacillus Veles solution; secondly, weigh 20 grams of culture medium, put it into 800 ml of water, mix well, divide into four parts, sterilize for 20 minutes, and then Add 3ml each of the obtained Lactobacillus acidophilus bacterial liquid, Bacillus subtilis bacterial liquid, Enterococcus bacterial liquid and Bacillus beilesi bacterial liquid to four parts of LB liquid respectively. 150r / min, expanded culture and adjustment, the concentration of Lactobacillus acidophilus bacteria solution was 0.3×88~1.0×108CFU / ml, the concentration of Bacillus subtilis bacteria solution was 3.5-6.1×1012CFU / ml, and the concentration of Enterococcus bacteria solution The concentration of Bacillus subtilis...

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PUM

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Abstract

The invention discloses a preparation method of a high-efficiency composite probiotic Bestroin for poultry intestinal tracts. According to the preparation method, lactobacillus acidophilus, bacillus subtilis, enterococcus and bacillus velezensis are respectively cultured in a culture base to obtain a lactobacillus acidophilus solution, a bacillus subtilis solution, an enterococcus solution and a bacillus velezensis solution; and the lactobacillus acidophilus solution, the bacillus subtilis solution, the enterococcus solution and the bacillus velezensis solution which are subjected to amplification culture are mixed to obtain a mixed bacterial solution. The preparation method of the high-efficiency composite probiotic Bestroin for poultry intestinal tracts has the advantages that the composite probiotic is applied to poultry feed, and achieves the effects of promoting digestion, softening excrement and the like; the immunity of poultry bodies can be enhanced, and good prevention and treatment effects are achieved on diseases caused by pathogenic bacteria in the poultry intestinal tracts; meanwhile, digestive tract, intestinal tract and respiratory tract diseases of the poultry can be prevented and treated; and healthy and rapid growth of the poultry is promoted.

Description

technical field [0001] The invention belongs to the field of biology, and in particular relates to a preparation method for high-efficiency compound probiotics of baccarinin used in poultry intestines. Background technique [0002] With the increasing intensification of the current farming industry, the development trend of poultry diseases is becoming more and more complex and changeable; poultry colibacillosis is a disease caused by pathogenic Escherichia coli, which is found in nature Widely distributed, such as air, dust, litter, feed, drinking water, poultry body surface, hatchery, incubator and other appliances in poultry houses, there are many poultry diseases, which will lead to low product quality, and the remaining poultry will cause food safety problems Threats to human health, and poultry feces contamination of eggs is the main source of infection in poultry, because the contaminated egg Escherichia coli can invade through the eggshell and shell membrane to cause...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12R1/23C12R1/125C12R1/07C12R1/01
CPCC12N1/20
Inventor 王昆
Owner 陆良佰佳益康生物科技有限公司
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