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Extraction method of tetrandrine A and tetrandrine B

A technology of tetrandrine B and tetrandrine A, which is applied in the field of material extraction, can solve the problems of cumbersome operation process, unfavorable environment and human body, and low extraction rate, and achieves the advantages of reducing pollution, shortening production cycle and improving extraction yield. Effect

Pending Publication Date: 2021-01-19
ZUNYI MEDICAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, the extraction and separation of tetrandrine and tetrandrine mostly use ethanol extraction, the extract is treated with acid and alkali, and then extracted with chloroform or acetone, or alkalized with ammonia water, etc., the operation process is cumbersome, the extraction rate is low, and the use of Highly toxic solvent, which is harmful to the environment and human body

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] After soaking 100g of Tetradandra powder (with a particle size of 45 mesh) with 100g of water for 1h, it was extracted by ultrasonic oscillation in 800g of ethanol solution with a volume concentration of 68%. first filtrate. Mix the first filter residue, water and enzyme preparation (composed of pectinase and cellulase with a weight ratio of 1:2) with a weight ratio of 10:20:0.02, and add acetic acid to the obtained mixed solution to adjust the pH value to 3.5, and then extracted by ultrasonic vibration at 40° C. for 3 h, and filtered to obtain the second filter residue and the second filtrate. The first filtrate and the second filtrate were mixed and concentrated to an extract. Mix the extract and absolute ethanol with a mass ratio of 1:5, and use an alumina chromatographic column for chromatographic purification. The chromatographic purification process uses dichloromethane with a volume ratio of 9:1, 7:1 and 5:1 in sequence. A mixture of methane and methanol was us...

Embodiment 2

[0042] Tetrandrine powder was extracted by ultrasonic vibration in an ethanol solution with a volume concentration of 55%, and other conditions were the same as in Example 1 to obtain tetrandrine.

[0043] The tetrandrine obtained in Example 2 had a purity of 99.5% and a yield of 0.78%.

Embodiment 3

[0045]After soaking 100g of Tetradandra powder (with a particle size of 50 mesh) with 100g of water for 1.5h, it was extracted by ultrasonic oscillation in 900g of ethanol solution with a volume concentration of 70%. The extraction temperature was 43°C and the time was 2.5h. Filter residue and first filtrate. Mix the first filter residue, water and enzyme preparation (composed of pectinase and cellulase with a weight ratio of 1:2.5) with a weight ratio of 10:25:0.03, and add acetic acid to the obtained mixed solution to adjust the pH value to 4.0, and then extracted by ultrasonic vibration at 43° C. for 2.5 h, and filtered to obtain the second filter residue and the second filtrate. The first filtrate and the second filtrate were mixed and concentrated to an extract. Mix the extract and absolute ethanol with a mass ratio of 1:7, and use an alumina chromatographic column for chromatographic purification. The chromatographic purification process uses dichloromethane with a volu...

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PUM

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Abstract

The invention belongs to the technical field of substance extraction. The invention provides an extraction method of tetrandrine A and tetrandrine B. The extraction method comprises the following steps: 1) performing extraction treatment on tetrandrine powder in an ethanol solution to obtain first filter residues and first filtrate; 2) carrying out extraction treatment on the first filter residue,water and an enzyme preparation to obtain a second filter residue and a second filtrate; 3) mixing the first filtrate and the second filtrate to obtain an extract; and 4) mixing the extract with absolute ethyl alcohol, and carrying out chromatographic purification by using an alumina chromatographic column to obtain tetrandrine A and / or tetrandrine B. The tetrandrine A and the tetrandrine B obtained by the extraction method disclosed by the invention are purely natural, non-irritant and free of cytotoxicity; according to the extraction method, the purity and the extraction yield of tetrandrine A and tetrandrine B are remarkably improved, the yield of tetrandrine A reaches 0.88% or above, and the yield of tetrandrine B reaches 0.78% or above.

Description

technical field [0001] The invention relates to the technical field of substance extraction, in particular to a method for extracting tetrandrine and tetrandrine. Background technique [0002] Fangji is the dry tuber of Fangji, a plant belonging to the genus Fangji, and its active ingredients are alkaloids, with a total alkaloid content of up to 2.3%, of which the main ones are tetrandrine (also known as tetrandrine) and tetrandrine ( Also known as fangchinoline base), both are natural bisbenzylisoquinoline alkaloids. Tetrandrine has analgesic, anti-tumor, anti-inflammatory, anti-arrhythmic, antibacterial, and antihypertensive effects. Tetrandrine B has anti-hepatic ischemia-reperfusion injury and induces apoptosis of human lung cancer 95D cells. [0003] At present, the extraction and separation of tetrandrine and tetrandrine mostly use ethanol extraction, the extract is treated with acid and alkali, and then extracted with chloroform or acetone, or alkalized with ammonia ...

Claims

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Application Information

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IPC IPC(8): C07D491/18
CPCC07D491/18
Inventor 付少彬孟庆峰张茂生闫松杨鸿强
Owner ZUNYI MEDICAL UNIVERSITY
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