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Immunodeficient mouse feed and preparation process thereof

A technology for immunodeficiency mice and feed, applied in animal feed, animal feed, food processing, etc., can solve problems affecting tumor and immunology research, difficulty in feeding and breeding, weak constitution of young mice, etc., to achieve good absorption and reproduction The effect of increasing the rate and reducing the difficulty

Pending Publication Date: 2021-01-22
北京华阜康生物科技股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to the special physiological characteristics of immunodeficiency mice, their feeding and breeding are more difficult than conventional strains, and there are common problems such as low reproductive ability, fast replacement of breed mice, and weak constitution of young mice. This is also a common problem faced by manufacturers. How to improve immunity The reproductive rate and animal quality of defective animals restrict the development of manufacturers, and directly affect the research of tumor and immunology

Method used

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  • Immunodeficient mouse feed and preparation process thereof

Examples

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preparation example 1

[0043] A kind of organozinc, its preparation steps are as follows:

[0044] 1) Add 0.5kg of iminodiacetonitrile to 10kg of tetrahydrofuran, stir until dissolved, raise the temperature to 50°C, and then add 0.45kg of epichlorohydrin dropwise at a rate of 0.5mL / min. After the dropwise addition, continue Stir the reaction for 3 hours, then add 1.9 L of 3 mol / L sodium hydroxide solution, stir the reaction for 2 hours, then dropwise add 3 mol / L hydrochloric acid solution, adjust its pH to 7, to obtain a mixed solution, and carry out rotary evaporation of the mixed solution, Obtain solid A;

[0045] 2) Add solid A to 20kg of distilled water, stir until dissolved, and then add 5.2kg of chitosan oligosaccharides. Chitosan oligosaccharides are added in 5 times on average, and the time interval between two adjacent additions is 30min. After the addition is complete, continue After stirring and reacting for 2 hours, solid sodium hydroxide was added to adjust its pH to 9 to obtain a mixe...

preparation example 2

[0049] A kind of organozinc, its preparation steps are as follows:

[0050]1) Add 0.6kg of iminodiacetonitrile to 12kg of tetrahydrofuran, stir until dissolved, raise the temperature to 55°C, and then add 0.50kg of epichlorohydrin dropwise at a rate of 1.0mL / min. After the dropwise addition, continue Stir the reaction for 3.5 hours, then add 2.2 L of sodium hydroxide solution with a concentration of 3.5 mol / L, stir and react for 2.5 hours, then add dropwise a 3.5 mol / L hydrochloric acid solution, adjust its pH to 8, and obtain a mixed solution. Perform rotary evaporation to obtain solid A;

[0051] 2) Add solid A to 25kg of distilled water, stir until dissolved, then add 5.5kg of chitosan oligosaccharides, the addition of chitosan oligosaccharides is divided into 6 times on average, and the time interval between two adjacent additions is 35min. After the addition is complete, continue After stirring and reacting for 2.5 hours, solid sodium hydroxide was added to adjust the pH...

preparation example 3

[0055] A kind of organozinc, its preparation steps are as follows:

[0056] 1) Add 0.7kg of iminodiacetonitrile to 15kg of tetrahydrofuran, stir until dissolved, raise the temperature to 60°C, and then add 0.55kg of epichlorohydrin dropwise at a rate of 1.5mL / min. After the dropwise addition, continue Stir the reaction for 4 hours, then add 2.0 L of sodium hydroxide solution with a concentration of 4 mol / L, stir the reaction for 3 hours, then add dropwise 3 mol / L hydrochloric acid solution, adjust its pH to 7, and obtain a mixed solution, which is rotatively evaporated, Obtain solid A;

[0057] 2) Add solid A to 30kg of distilled water, stir until dissolved, and then add 6.0kg of chitosan oligosaccharides. Chitosan oligosaccharides are added in 7 times on average, and the time interval between two adjacent additions is 40min. After the addition is complete, continue After stirring and reacting for 3 hours, solid sodium hydroxide was added to adjust its pH to 9 to obtain a mix...

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Abstract

The invention relates to the field of laboratory mouse feed, and particularly discloses immunodeficient mouse feed. The immunodeficient mouse feed is prepared from the following raw materials in partsby weight: 80-90 parts of corn flour, 4-8 parts of bran, 5-9 parts of soybean meal, 1-2 parts of beer yeast, 5-8 parts of vegetable oil, 0.001-0.003 part of organic zinc, 0.001-0.002 part of selenoprotein, 0.2-0.5 part of methionine, 0.2-0.5 part of lysine, 0.1-0.2 part of vitamin A, 0.1-0.2 part of vitamin B6, 0.01-0.02 part of ferrous sulfate and 0.08-0.12 part of calcium bicarbonate, wherein the organic zinc is prepared as follows: iminodiacetonitrile reacts with epoxy chloropropane, then an obtained product reacts with chitosan oligosaccharide, a generated compound is hydrolyzed, and thencoordination reaction with zinc ions is conducted. The immunodeficient mouse feed has the advantage of improving the reproductive rate of immunodeficient mice. In addition, the invention further provides a preparation method of the immunodeficient mouse feed.

Description

technical field [0001] This application relates to the field of experimental mouse feed, more specifically, it relates to a kind of immunodeficiency mouse feed and its preparation process. Background technique [0002] Tumor and immunology research requires a large number of immunodeficient mice, which have no immune system and low resistance. For users, the higher the degree of immunodeficiency of mice, the higher the survival rate of tumor inoculation, but at the same time the weaker their constitution, the lower their resistance and reproductive rate. Due to the special physiological characteristics of immunodeficiency mice, their feeding and breeding are more difficult than conventional strains, and there are common problems such as low reproductive ability, fast replacement of breed mice, and weak constitution of young mice. This is also a common problem faced by manufacturers. How to improve immunity The reproductive rate and animal quality of defective animals restri...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23K10/16A23K10/30A23K10/37A23K20/142A23K20/147A23K20/158A23K20/174A23K20/20A23K20/24A23K50/50C08B37/08
CPCA23K10/16A23K10/30A23K10/37A23K20/142A23K20/147A23K20/158A23K20/174A23K20/24A23K20/30A23K50/50C08B37/003Y02P60/87
Inventor 刘云波
Owner 北京华阜康生物科技股份有限公司
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