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Sinorhizobium sp. strain with phosphorus-dissolving capacity and application thereof

A technology of Sinorhizobia and strains, applied in the direction of application, bacteria, organic fertilizers, etc., to achieve the effect of improving growth conditions and increasing available phosphorus content

Active Publication Date: 2021-01-22
INST OF SOIL SCI CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are few reports on phosphorus-solubilizing bacteria that are suitable for acidic red soil and promoting crop production. How to use phosphorus-solubilizing bacteria to make bacterial agents to improve the activation and release of insoluble phosphorus in soil has important application value

Method used

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  • Sinorhizobium sp. strain with phosphorus-dissolving capacity and application thereof
  • Sinorhizobium sp. strain with phosphorus-dissolving capacity and application thereof
  • Sinorhizobium sp. strain with phosphorus-dissolving capacity and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Screening and Identification of Sinorhizobium.sp Strain YTR-S1

[0026] 1. Screening process

[0027] (1) Weigh 1g of corn rhizosphere soil and put it into a centrifuge tube, add 9mL of 0.9% sterilized normal saline to make 10 -1 Put the bacterial suspension in a shaking box at 180r / min for 10min, take it out and let it stand for 1h.

[0028] (2) Draw 5 μL and add 45 μL sterile water from the soil suspension after standing to make 10 -2 bacterial suspension. The bacterial suspension was diluted to 10 -3 、10 -4 、10 -5 , respectively take 5ml 10 -5 The bacterial suspension was added to 50ml and 500mL of KB medium to make 1 - 0 -6 and 10 -7 For the culture medium, 250 μL of 10 -6 and 10 -7 The culture medium was added to the 96-well plate. Finally, the 96-well plate was placed in a microplate reader, and its growth was observed at room temperature.

[0029] (3) Take 200 μL of the pore size of the bacteria grown in the plate and put it into a preservation tube,...

Embodiment 2

[0032] Phosphorus Solubilizing Properties of Sinorhizobium.sp Strain YTR-S1

[0033] (1) Use the phosphorus circle method. The organic phosphorus source is lecithin, and the inorganic phosphorus source is aluminum phosphate and iron phosphate, both of which are commercially available analytical reagents.

[0034] (2) Prepare Montina organophosphate (lecithin), aluminum phosphate, and iron phosphate medium plates. Each plate is divided into 6 areas, and 2 μL points are sequentially drawn in the center of each area, and cultured in a 28°C incubator for 3 days. Observe whether the strain grows and forms a phosphorus-dissolving circle, and record the diameter of the phosphorus-dissolving circle (unit D / cm) and the diameter of the colony (unit d / cm), and calculate the D / d ratio to determine the solution of different insoluble phosphorus by the strain. Phosphorus capacity and phosphorus solubilization efficiency. The formula of the culture medium is as follows: ①Mengkina organic p...

Embodiment 3

[0038] Corn pot experiment

[0039] 1. There are two treatments in this experiment, the application of Sinorhizobium YTR-S1 and the control treatment (CK) without bacterial agent, and each treatment is repeated 5 times.

[0040] 2. Soil and fertilizer dosage: according to the organic fertilizer content (mass content) 2% in the soil, fully mix the organic fertilizer with the red soil, sterilize, and put it into a plastic pot with an upper diameter of 10 cm, a lower diameter of 7 cm, and a height of 8.5 cm. Soil matrix 500g.

[0041] 3. Seedling cultivation: the corn seed is Suyu 24. 1) Seed disinfection: first disinfect with 70% alcohol for 1 min, then disinfect with 1% sodium hypochlorite for 15 min, and finally rinse with sterile water five times. Put the sterilized seeds on sterile water, place them in an incubator at 28° C. for dark cultivation and wait for them to germinate; 2) select seedlings with consistent growth and transplant them into potted red soil on the fifth ...

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Abstract

The present invention discloses a sinorhizobium sp. strain with phosphorus-dissolving capacity. The strain is the sinorhizobium sp. strain YTR-S1 and preserved in China General Microbiological CultureCollection Center on September 18, 2020, and has the preservation number of CGMCC 20681. Based on an organic fertilization long-term positioning test of a Yingtan red soil ecological experiment station of Chinese Academy of Sciences, an efficient phosphorus-dissolving bacterium is screened from corn rhizosphere soil. Sequencing identification is performed on the efficient phosphorus-dissolving bacterium. The strain YTR-S1 has capacity of efficiently dissolving inorganic phosphorus and organic phosphorus. A potting experiment finds that the added strain YTR-S1 remarkably increases available phosphorus content of red soil and improves growth condition and quality of corn.

Description

technical field [0001] The invention belongs to the technical field of agricultural microorganisms, and in particular relates to a Sinorhizobium strain and its application in phosphorus solubilization. Background technique [0002] Phosphorus (P) is one of the important element indicators to measure soil fertility, and it is also an essential element for metabolic processes such as protein and phosphoric acid synthesis and energy cycle in plants. The dry red soil in the southeastern hilly area is an important base for tropical and subtropical economic fruit, cash crops and grain production in my country, and plays an important role in the sustainable development of agriculture and the construction of ecological environment in China. The amount of total phosphorus in red soil is relatively high, of which inorganic phosphorus accounts for 35%-70% of total phosphorus, organic phosphorus accounts for 30%-65% of total phosphorus, but a large amount of phosphorus is covered by met...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A01N63/20A01P21/00C09K17/14C12R1/41C09K101/00
CPCA01N63/20C09K17/14C09K2101/00C12N1/20C12N1/205C12R2001/41
Inventor 蒋瑀霁孙波陈紫云郑洁孔培君
Owner INST OF SOIL SCI CHINESE ACAD OF SCI
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