Preparation method of H2O2 self-supply type calcium peroxide loaded curcumin nanoparticles and application thereof

A calcium peroxide, H2O2 technology, applied in the field of biomedicine, can solve problems such as no preparation method of curcumin nanoparticles, and achieve the effects of enhancing anti-tumor effect, scientific and reasonable preparation method and low cost

Active Publication Date: 2021-01-29
ZHENGZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In this way, the mechanism of calcium overload, chemotherapy, chemodynamic therapy and other mechanisms can achieve the effect of triple-strike interactive comprehensive treatment on tumors, but so far no H 2 o 2 Public report on the preparation method and application of self-supply calcium peroxide-loaded curcumin nanoparticles

Method used

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  • Preparation method of H2O2 self-supply type calcium peroxide loaded curcumin nanoparticles and application thereof

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] In the specific implementation of the present invention, a kind of H 2 o 2 The preparation method of self-supply type calcium peroxide loaded curcumin nanoparticles, comprises the following steps:

[0016] (1) Add 1.8mg of calcium chloride particles to 12.5mL of ultrapure water, stir at 2000rpm to dissolve and filter, add 7mL of 1M ammonia water to the filtrate while stirring at room temperature, and add 90mL of PEG800 at the same time, continue to stir to make it Mix evenly, then add 6mL of 30% hydrogen peroxide to the solution and continue to stir for 12h, then gradually adjust the pH to 11.5 with sodium hydroxide solution, centrifuge at 12500rpm for 15min, discard the supernatant, and obtain a white precipitate, which can be used in 7mL without Washed with water and ethanol for 3 times, each time for 7min, and dried to obtain calcium peroxide (CaO 2 ) nanoparticles;

[0017] (2), 15mg calcium peroxide (CaO 2 ) nanoparticles and 8 mg of positively charged transfer...

Embodiment 2

[0020] In the specific implementation of the present invention, a kind of H 2 o 2 The preparation method of self-supply type calcium peroxide loaded curcumin nanoparticles, comprises the following steps:

[0021] (1) Add 1.5mg of calcium chloride particles to 10mL of ultrapure water, stir at 1800rpm to dissolve and filter, add 8mL of 0.5M ammonia water to the filtrate while stirring at room temperature, and add 80mL of PEG800 at the same time, continue to stir to make it Mix well, then add 5mL of 30% hydrogen peroxide to the solution and continue to stir for 12h, then gradually adjust the pH to 11.5 with sodium hydroxide solution, centrifuge at 10000rpm for 15min, discard the supernatant, and obtain a white precipitate, which can be used with 5mL of Washed with water and ethanol for 3 times, each time for 5min, and dried to obtain calcium peroxide (CaO 2 ) nanoparticles;

[0022] (2), 10mg calcium peroxide (CaO 2 ) nanoparticles and 5mg positively charged transferrin were ...

Embodiment 3

[0025] In the specific implementation of the present invention, a kind of H 2 o 2 The preparation method of self-supply type calcium peroxide loaded curcumin nanoparticles, comprises the following steps:

[0026](1) Add 2mg of calcium chloride particles to 15mL of ultrapure water, stir at 2200rpm to dissolve and filter, add 6mL of 1.5M ammonia water to the filtrate while stirring at room temperature, and add 100mL of PEG800 at the same time, continue to stir to mix Evenly, then add 7mL of mass concentration 30% hydrogen peroxide to the solution and continue to stir for 12h, then gradually adjust the pH to 11.5 with sodium hydroxide solution, centrifuge at 15000rpm for 15min, discard the supernatant to obtain a white precipitate, and use 8mL of anhydrous Wash with ethanol 3 times, each time for 8min, and dry to obtain calcium peroxide (CaO 2 ) nanoparticles;

[0027] (2), 20mg calcium peroxide (CaO 2 ) nanoparticles and 10 mg of positively charged transferrin were added to ...

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Abstract

The invention relates to a preparation method and an application of H2O2 self-supply type calcium peroxide loaded curcumin nanoparticles, which can effectively solve the problems of preparation of theH2O2 self-supply type calcium peroxide loaded curcumin nanoparticles, calcium overload aiming at tumors, chemotherapy, chemodynamic therapy and drug use for promoting tumor cell apoptosis, the particle size of calcium peroxide is 50-100nm, the pore diameter is 5-8nm, transferrin is loaded in holes of calcium peroxide through electrostatic adsorption, the transferrin is positively charged, the mass ratio of the calcium peroxide to the transferrin is 3-5: 1, and then a micromolecular antitumor drug curcumin is loaded on the surface of the transferrin through electrostatic adsorption, so that H2O2 self-supplied calcium peroxide loaded curcumin nanoparticles with the drug loading weight of 30%-55% and the particle size of 55-105 nm are obtained. The preparation method disclosed by the invention is scientific, reasonable, stable, reliable and low in cost, and the prepared H2O2 self-supplied calcium peroxide loaded curcumin nanoparticles can play a role in triple strike interaction comprehensive treatment of tumors in the aspect of preparation of anti-tumor drugs, so that the anti-tumor effect is enhanced.

Description

technical field [0001] The invention relates to the field of biomedicine, in particular to a H 2 o 2 Preparation method and application of self-supply calcium peroxide-loaded curcumin nanoparticles. Background technique [0002] In recent years, ion-based interference therapy has become a hot spot for researchers. The homeostasis of various ions plays a pivotal role in the metabolism and proliferation of cells in the human body. Studies have shown that Ca 2+ It is the second messenger of cells and participates in the transduction of apoptosis signals. If extracellular Ca 2+ Influx or release of intracellular calcium stores can lead to intracellular calcium overload. Calcium peroxide (CaO 2 ) nanoparticles can generate a large amount of Ca in the weakly acidic environment of the tumor 2+ and H 2 o 2 , leading to oxidative stress and calcium overload. Ca 2+ The abnormal accumulation of β not only induces apoptosis, but also promotes the development of calcification ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K9/51A61K31/12A61K33/40A61K33/00A61K47/64A61P35/00
CPCA61K9/5192A61K31/12A61K33/40A61K33/00A61K47/644A61P35/00A61K2300/00
Inventor 冯倩华张雪莉陈静梅玉影王宁郝雨桐王泽颖张振中
Owner ZHENGZHOU UNIV
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