Multi-frequency point resonance biosensor, its preparation method and use thereof in cell concentration detection

Abstract

The present invention provides a multi-frequency point resonance biosensor, a preparation method thereof and a method for detecting cell concentration with the biosensor. The multi-frequency point resonance biosensor comprises a plurality of basic units. The basic unit comprises a metal layer and a dielectric layer: the metal layer is composed of an asymmetric U-shaped structure and a rectangularantenna structure. The metal layer comprises a metal upper layer and a metal lower layer, wherein the metal upper layer is made of gold, and the metal lower layer is made of titanium. The dielectric layer comprises polyimide film. The biosensor of the invention can realize high-sensitivity cell sensing with fast and multi-resonance label-free detection in the terahertz frequency.

Description

technical field [0001] The invention relates to the intersection field of terahertz technology and biotechnology, in particular to a terahertz non-bianisotropic high-order mode Fano resonance multi-frequency point resonant metamaterial label-free biosensor, a preparation method thereof, and a method for testing cell concentration. Background technique [0002] The development of terahertz technology has attracted worldwide attention and has become one of the most important core technologies in the new century. Many countries have listed terahertz technology as a key research and development project. Due to the unique electric field response, terahertz technology is widely used in public safety, communication, and biomedicine. The current methods for detecting cell concentration mainly include labeled fluorescence detection and labeled flow cytometry. Although these have very high sensitivity in practical applications, their detection costs are very high, and most of them ar...

AI Technical Summary

Problems solved by technology

The current methods for detecting cell concentration mainly include labeled fluorescence detection and labeled flow cytometry. Although these have very high sensitivity in practical applications, their detection costs are very high, and most of them are used in conjunction with other chemical substances, which in turn will cause cause a certain amount of pollution

For example: CCK-8 method is sensitive to detection, but CCK-8 is expensive. In addition, the color of CCK-8 reagent is light red, which is similar to the color of the medium, and it is easy to cause incorrect operations such as missing or adding more in the experiment; There is also flow cytometry, which uses flow cytometry to quantitatively detect and analyze a single cell. It integrates a series of technologies such as monoclonal antibodies, immunocytochemistry, laser and computer science, and has a fast detection speed. , high sensitivity, but also can not avoid the need for marking, high cost, long time-consuming and other problems

There is currently no low-cost, label-free cell detection solution

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