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Peripheral blood TCR marker for novel coronavirus infection and detection kit and application thereof

A coronavirus and marker technology, applied in the field of genetic engineering, can solve the problem of short maintenance time of IgM antibody response, and achieve the effects of low cost, low labor cost, high specificity and accuracy

Pending Publication Date: 2021-02-05
SICHUAN PROVINCIAL PEOPLES HOSPITAL +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Generally, 5-7 days after infection with the new coronavirus, the human body will produce a virus antigen-specific IgM antibody response, but the IgM antibody response lasts for a short time and disappears soon

Method used

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  • Peripheral blood TCR marker for novel coronavirus infection and detection kit and application thereof
  • Peripheral blood TCR marker for novel coronavirus infection and detection kit and application thereof
  • Peripheral blood TCR marker for novel coronavirus infection and detection kit and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0051] Through immune map analysis, the new coronavirus infection TCR marker CDR3 sequence set was obtained:

[0052] 1. Sampling and immune map analysis

[0053] Collect 1161 control groups (including healthy people and patients not infected with the new crown, 1160 people for model building, 1 healthy person for verification), 81 patients with new coronavirus infection (80 people for model building, 1 person for for verification) and the peripheral blood of a subject with unknown health status (10mL per person), the amino acid sequence of the TCR epitope 3 (CDR3) of the subject and the control group was obtained by high-throughput sequencing, ensuring that each sample The total number of CDR3 sequences of the functional TCR is not less than 30,000;

[0054] 2. The CDR3 sequence of the TCR of each sample is randomly sampled without replacement, so that the total number of CDR3 sequences of each sample is 30,000. For any specific CDR3 sequence X, the number of repeated occur...

Embodiment 2

[0061] Verify the specificity of the new coronavirus infection TCR marker CDR3 sequence set:

[0062] 1. Sampling and immune map analysis

[0063] The peripheral blood (10mL per person) of 224 tumor patients not infected with the new crown and 5 subjects with unknown health status was collected, and the epitope 3 (CDR3) amino acid of the TCR of the subjects and the control group was obtained by high-throughput sequencing Sequences, to ensure that the total number of CDR3 sequences of functional TCRs in each sample is not less than 30,000; random non-replacement sampling is performed on the CDR3 sequences of TCRs in each sample, so that the total number of CDR3 sequences in each sample is 30,000.

[0064] 2. Randomly select 100 healthy people and 45 non-tumor disease patients from the control group in Example 1.

[0065] 3. According to the 100 healthy people from Example 1, 45 patients with non-tumor diseases who were not infected with the new crown, and 80 patients with new ...

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Abstract

The invention discloses a peripheral blood TCR marker for novel coronavirus infection as well as a detection kit and application thereof. The peripheral blood TCR marker comprises at least one of proteins shown in a sequence SpecSeq1-100. Based on a high-throughput sequencing method, only a small amount of peripheral blood needs to be adopted, RNA needs to be extracted, an immune map library is established by processing a sample, then through high-throughput sequencing and TCR data analysis, a characteristic TCR sequence in the peripheral blood infected by the novel coronavirus is determined firstly, then a test result of the sample to be tested is compared with the characteristic TCR sequence, and therefore, whether the patient suffers from the novel coronavirus infection or not is determined. According to the peripheral blood TCR marker for the novel coronavirus infection and the detection kit and application thereof, the huge number of the novel coronavirus infection specific TCR sequences can be simultaneously compared, the specificity and the accuracy are high compared to the single detection of one or more markers, and the diagnosis efficiency is improved.

Description

technical field [0001] The invention belongs to the technical field of genetic engineering, and in particular relates to a peripheral blood TCR marker for novel coronavirus infection, a detection kit and application thereof. Background technique [0002] The new type of coronavirus (SARS-Cov-2) is highly contagious and harmful, and there is currently no specific drug for treatment. The number of confirmed cases of new coronavirus infection worldwide has exceeded 10 million, resulting in 500,000 deaths. [0003] At present, the diagnostic methods for 2019-nCoV infection mainly include viral nucleic acid detection, serological IgM and IgG detection, and bilateral lung CT. The nucleic acid detection of the new coronavirus mainly relies on the PCR amplification reaction, which can detect whether there is a virus nucleic acid gene in the sample to be tested, so it is the standard for detecting the new coronavirus. Serological IgM and IgG detection is by detecting the humoral im...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K14/725C07K16/28G01N33/68C40B40/10
CPCC07K14/7051C07K16/2809G01N33/6818G01N33/6893C40B40/10G01N2800/26
Inventor 张志新王福生张毅邓绍平卓越张纪元杨鑫
Owner SICHUAN PROVINCIAL PEOPLES HOSPITAL
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