Specific human gene fragment and primer probe and application thereof

A gene fragment, primer probe technology, applied in the field of molecular biological detection, can solve the problems of inability to specifically detect a variety of animal and human genes, lack of specificity of target genes, and inability to directly correspond to cell volume, etc., to reduce costs and operation. Simple, time-saving effects
CN112322706AInactive Publication Date: 2021-02-05北京昭衍新药研究中心股份有限公司
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
北京昭衍新药研究中心股份有限公司
Publication Date
2021-02-05
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention provides a human cell specific gene fragment, a specific primer and a probe thereof, and a method for specific quantitative detection of a human gene. According to the invention, throughthe NCBI website and a BLAST sequence comparison function of the NCBI website, a human specific gene fragment with a length of 166 bp on FOXP2 gene is obtained, and human genes in tissues, blood andother non-human samples of various preclinical animals are quantified by qPCR. The human cell specific gene fragment and qPCR quantitative detection method adopted by the invention are high in specificity, strong in sensitivity, good in repeatability and simpler and more convenient to operate, and provide a new thought and a corresponding technical support for quantitative detection of specific human genes.
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Description

technical field

[0001] The invention relates to the technical field of molecular biological detection, in particular to a specific human gene fragment, primers, probes and applications thereof. Background technique

[0002] In recent years, the administration of cell therapy drugs such as mesenchymal stem cells, hematopoietic stem cells, embryonic stem cells, and immune cells has become a research hotspot in the fields of tumors, diabetes, and various organ function declines, and many innovative drugs have entered the clinical trial stage , showing great application potential of cell therapy drugs. At present, many cell therapy drugs are still in the preclinical research stage, and there is still a lack of sufficient understanding of the distribution, metabolism, and colonization and differentiation of stem cells in vivo after cell drugs enter the body. The distribution and metabolism of cellular drugs in the body have a direct impact on their efficacy, so preclinical pharm...

Claims

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