Polyarylthiopyridine positive ion salt light-controlled cell pyroptosis material as well as preparation method and application of polyarylthiopyridine positive ion salt light-controlled cell pyroptosis material
A technology of arylthiopyridine and ylthiopyridine, which is applied in the field of polyarylthiopyridine positive ion salt light-controlled pyroptosis materials and its preparation, can solve the problem of unreachable pyroptosis and uncontrollable pyroptosis process and other issues, to achieve the effects of easy large-scale commercialization, good biocompatibility, and cheap and easy-to-obtain raw materials
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Embodiment 1
[0030] Embodiment 1 (synthesis of photoluminescence material)
[0031] The synthesis of compound 1, the specific steps are as follows:
[0032] Add 12 mmol of ethyl 4-mercaptobenzoate, 1 mmol of hexachlorobenzene into 20 mL of DMF solvent, and simultaneously add 24 mmol of K 2 CO 3 , reacted at 60°C for 60 hours under a nitrogen atmosphere, and stopped the reaction; when cooled to room temperature, 100 mL of distilled water was added to produce a yellow precipitate, which was purified by column chromatography after filtration, and the eluent was ethyl acetate and petroleum ether (1:3 ) to obtain compound 1 with a yield of 72%.
[0033] The synthesis of compound 2, the specific steps are as follows:
[0034] Add 20 mL of THF to 1 mmol of compound 1 to dissolve it, then add 20 mL of 1.5 M sodium hydroxide aqueous solution, and react at room temperature for 24 hours; after the reaction, add 30 mL of 2 M hydrochloric acid to precipitate an orange-yellow precipitate; After filt...
Embodiment 2
[0042] Example 2 (macromolecule binding luminescent phenomenon)
[0043] Take the solution of the concentration obtained in step 3, dilute it 10 times with deionization, take 3 mL in a cuvette, detect the emission peak in the wavelength range of 385 nm ~ 600 nm, and obtain the absorption spectrum under 365 nm light as figure 1 As shown in the gray curve of the emission spectrum, the solution emits almost no light. Take 1 mL of the diluted solution, add 0.5 mg of DNA aqueous solution dropwise to it continuously, place the mixed solution in a cuvette, use 365 nm as the excitation wavelength, and detect the emission spectrum between 385 nm and 600 nm. The maximum emission wavelength was obtained at 535 nm, and the luminous intensity increased significantly with the increase of DNA content ( figure 2 ).
Embodiment 3
[0044] Example 3 (specific markers)
[0045] The solution with the concentration obtained in step 3 was diluted 100 times, and the standard mitochondrial fluorescent probe was added to Hela cells containing 10 μM for culture, and laser confocal imaging was used. The cultured cells were illuminated and imaged. At this time, it can be seen that the position of the compound 4 solution in the cell is completely consistent with the position of the mitochondria, indicating that the compound 4 can specifically reach the mitochondria of the cell ( figure 2 ).
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