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Bone tissue engineering scaffold material for treating maxillofacial bone defects and synthesis method of bone tissue engineering scaffold material

A technology of bone tissue engineering and scaffold materials, applied in tissue regeneration, prosthesis, medical science, etc., can solve the problem of inability to achieve sustainable, stable and efficient scaffold materials, no significant improvement in mechanical properties of scaffold materials, unstable pectin structure and composition, etc. problem, achieve the effect of improving mechanical strength, simple preparation method, and promoting osteogenic differentiation

Inactive Publication Date: 2021-02-19
TONGJI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This patent has the following disadvantages: 1. In terms of composition, this patent uses pectin as one of the components of the scaffold. Due to the changes in plant species, storage time and processing technology, the structure and composition of pectin are very unstable and cannot be sustained. The preparation of stable and efficient scaffold materials, and due to the immature pectin production process, only citrus peels and apple peels are currently used as raw materials to produce pectin that can meet the requirements of commercial production
2. Functionally, the addition of pectin does not significantly improve the mechanical properties of scaffold materials

Method used

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  • Bone tissue engineering scaffold material for treating maxillofacial bone defects and synthesis method of bone tissue engineering scaffold material
  • Bone tissue engineering scaffold material for treating maxillofacial bone defects and synthesis method of bone tissue engineering scaffold material
  • Bone tissue engineering scaffold material for treating maxillofacial bone defects and synthesis method of bone tissue engineering scaffold material

Examples

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Effect test

preparation example Construction

[0050] A synthetic method of bone tissue engineering scaffold material as described above, comprising the following steps:

[0051] (1) Mix gelatin and water and heat to dissolve at 50-65°C to form a gelatin solution. The gelatin solution is placed at a temperature of ≤4°C for later use. The mass-to-volume ratio of gelatin and water is 1g / 10ml;

[0052] (2) Get chitosan, water and acetic acid solution and mix and dissolve at room temperature to form a suspension of chitosan. The suspension of chitosan is placed at a temperature of ≤4°C for subsequent use. The mass volume ratio of solution is 1g / 20ml, and the volume fraction of acetic acid solution is 2%;

[0053] (3) Take polyvinyl alcohol and water to dissolve by heating at 90-95°C to form a polyvinyl alcohol solution, and place the polyvinyl alcohol solution at a temperature of ≤4°C for later use, and the mass-to-volume ratio of polyvinyl alcohol to water is 6g / 100ml;

[0054] (4) Mix the gelatin solution, chitosan suspensi...

Embodiment 1

[0057] A bone tissue engineering scaffold material for treating maxillofacial bone defects, such as figure 1 As shown, the active ingredients include gelatin (gel), chitosan (cs), polyvinyl alcohol (pva) and nano-hydroxyapatite (nHA). The composition and structure of this material simulate natural bone tissue, and the mass ratio (w / w) of each effective component is 2:2:3:1 (gelatin: chitosan: polyvinyl alcohol: nano-hydroxyapatite) , after adding a volatile cross-linking agent, a porous composite scaffold structure that can effectively promote bone defect repair is finally formed.

[0058] The synthesis method of the bone tissue engineering scaffold material for the treatment of maxillofacial bone defects includes the following steps:

[0059] (1) Preparation of gelatin solution: Weigh a certain amount of gelatin (gel) (gel strength of the gelatin is 250 bloom g), add appropriate amount of distilled water to ensure that the mass-volume ratio (w / v) is 1g / 10ml, and then put it ...

Embodiment 2

[0066]A bone tissue engineering scaffold material for the treatment of maxillofacial bone defects, the synthesis method used is the same as that of Example 1 except for gel:cs:pva:nHA=2:2:3:7, the obtained material Denoted as MB-HA50%, the obtained material has been tested by universal force, the compression strength of the material is 1.71Mpa, the elastic modulus is 3.73Mpa, the swelling ratio is 4.05, the porosity of the material is 71%, the pore diameter of the material is 100um, nanometer The particle size of hydroxyapatite is 10-100nm, MB-HA50% prepared in this example is used to induce osteogenic differentiation of rat bone marrow mesenchymal stem cells, the results are as follows Figure 6 As shown, the expression level of Runx2 in MB-HA50% was 0.0046.

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Abstract

The present invention relates to a bone tissue engineering scaffold material for treating maxillofacial bone defects and a synthesis method of the bone tissue engineering scaffold material. The scaffold material is a gelatin-chitosan-based scaffold material and comprises gelatin, chitosan, polyvinyl alcohol and nano-hydroxyapatite in a mass ratio of (1.5-2.5):(1.5-2.5):(3-6):(1-7). The synthesis method comprises the following steps: (1) taking gelatin and water to form a gelatin solution; (2) taking chitosan, water and an acetic acid solution to form a chitosan turbid solution; (3) taking polyvinyl alcohol and water to form a polyvinyl alcohol solution; (4) mixing the gelatin solution, the chitosan turbid liquid and the polyvinyl alcohol solution, adding nano-hydroxyapatite, and performingstirring and cooling to a room temperature to obtain a material precursor solution; and (5) freezing the material precursor solution and performing freeze-drying, cross-linking and repeatedly freeze-drying. Compared with the prior art, the scaffold material has advantages of good osteogenesis promoting capability, excellent mechanical strength, good biocompatibility, low toxicity and small side effect, and provides a new material choice for repairing maxillofacial bone defects.

Description

technical field [0001] The invention belongs to the field of biological materials, and in particular relates to a bone tissue engineering scaffold material for treating maxillofacial bone defects and a synthesis method thereof. Background technique [0002] The oral and maxillofacial area is responsible for many important physiological functions such as mastication, swallowing, and language, among which the maxillofacial bone is an important bony support that supports the shape and function of the maxillofacial area. Bone defects caused by maxillofacial trauma, tumors, periodontal disease and other inflammation or other anatomical factors not only affect the appearance of patients, but also have a bad impact on patients' mental health. Achieving fast and good repair of maxillofacial bone defects has positive significance for the development of other follow-up treatments and healthy life for patients. [0003] Compared with other parts of the body, the bone of the maxillofac...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/22A61L27/20A61L27/16A61L27/12A61L27/50A61L27/56A61L27/58
CPCA61L27/222A61L27/20A61L27/16A61L27/12A61L27/50A61L27/56A61L27/58A61L2430/02C08L5/08C08L29/04
Inventor 马鹏飞林淑贤吴珺华
Owner TONGJI UNIV
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