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Cucumis sativus grafting method and application thereof in meloidogyne spp. control

A technology of root-knot nematode and root-knot nematode, applied in grafting, fruit tree cultivation and other directions, can solve the problem of low level of resistance of pumpkin to root-knot nematode, and achieve rich choices, reduce use, improve income and product quality. Effect

Pending Publication Date: 2021-02-23
甘肃省农业科学院蔬菜研究所
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Researchers conducted a large number of nematode resistance identifications on cucurbit crops and found that there are very few pumpkin germplasm resources resistant to M. incognita, and the resistance level of squash to M. incognita is low

Method used

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  • Cucumis sativus grafting method and application thereof in meloidogyne spp. control
  • Cucumis sativus grafting method and application thereof in meloidogyne spp. control
  • Cucumis sativus grafting method and application thereof in meloidogyne spp. control

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] Example 1, Screening and identification of root-knot nematode resistant cucumber germplasm resources

[0039] 1. Collection of germplasm resources

[0040] Collected 110 germplasm resources of the genus Cucumber from home and abroad, including 4 North China type cucumber materials, 2 South China type cucumber materials, 2 fruit cucumber materials, 2 white cucumber materials, 50 cucumber wild resources, interspecific hybrid introgression There are 46 copies of materials and 4 copies of semi-wild resources. Some representative materials are listed in Table 1.

[0041] 2. Identification of nematode pathogens and preparation of inoculum

[0042] (1) Identification and propagation of nematode pathogens

[0043] Cucumber roots infected with root-knot nematodes were collected from the field, and light yellow, large and plump high-quality single egg masses were picked from the cucumber root knots, inoculated into the infected tomato roots pre-cultivated in sterilized soil, a...

Embodiment 2

[0065] Embodiment 2, the grafting adaptability test of anti-root-knot nematode germplasm

[0066] 1. Seedling cultivation

[0067] 2 rootstocks, which are the selected Cucumis metuliferus lines PI 482443 and PI482452, and 3 scions, including dense thorn cucumber 'Jinlu 3', white cucumber 'Baiye San', and fruit cucumber 'corn Mini'. The rootstock was raised in a nutrient pot, and the scion was directly sown on the seedbed. The rootstock was sown 20 days earlier than the scion. After emergence, normal fertilizer and water management was carried out to cultivate strong seedlings.

[0068] 2. Screening of grafting methods

[0069] Through the screening of suitable grafting methods, two methods of grafting and cleaving were determined to be used for grafting.

[0070] Insertion: first dig out the true leaves and growth points of the rootstock, and use a bamboo stick to insert obliquely from the base of the main vein of one cotyledon to the other cotyledon to a depth of 5-7 mm; t...

Embodiment 3

[0079] Embodiment 3, the application of anti-root-knot nematode cucumber stock

[0080] 1. Selection of test site

[0081] The field experiment was arranged in the solar greenhouse of Shun'an Village, Fafa Town, Liangzhou District, Wuwei City, Gansu Province. The plot is a cucumber stubble field. Cucumbers have been planted continuously for 5 years. The occurrence of root-knot nematodes is serious. The average density of root-knot nematodes in the soil is 71.3 / 100g dry soil.

[0082] 2. Test treatment

[0083] A total of 6 treatment combinations were set up in the experiment, among which No. 1, No. 2 and No. 3 were grafting cultivation. The rootstocks were black-seeded pumpkin, Cucumis metuliferus (Cucumis metuliferus) strains PI 482443 and PI 482452, and the scions were Jinlu No. 3. The whole growing season No nematicides were applied, No. 4, 5, and 6 were self-rooted cultivation of Jinlv No. 3, No. 4 did not apply nematocides, No. 5 put plant nematode powder in the planti...

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Abstract

The invention discloses a cucumis sativus grafting method and application thereof in meloidogyne spp. control. The invention relates to a cucumis sativus grafting method. The cucumis sativus graftingmethod comprises the following steps that cucumis metuliferus (cucumis metuliferus) with meloidogyne spp. resistance is used as a rootstock, and cucumis sativus (cucumis sativus) is used as a scion for grafting. According to the method, co-rootstock grafting of the cucumis sativus is achieved, the selection range of the cucumis sativus rootstock is enriched, the obvious yield increasing effect isachieved, the control efficiency on the meloidogyne spp. is remarkably superior to that of pumpkin grafting and nematicide, and meanwhile the flavor and quality of grafted cucumis sativus are improved; and in addition, by using the method to control the meloidogyne spp., the use of the nematicide is reduced, the production cost is reduced, the pesticide residue is reduced, the cucumis sativus planting income and the product quality are improved, and the sustainable development of the cucumis sativus industry is guaranteed.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a cucumber grafting method and its application in the prevention and treatment of root-knot nematodes. Background technique [0002] Root-knot nematode (Meloidogyne spp.) is an important pathogen that harms the production of cucumber (Cucumis sativus L., 2n=14), causing significant losses to global cucumber production, with a reduction of 10% to 15% in normal years and 30% to 40% in severe cases. %, or even no harvest, has become a serious obstacle affecting cucumber production. The wounds caused by the nematode invasion of the root system can also cause the compound infection of cucumber wilt (Fusarium oxysporum), root rot (Pythium aphanidermatum) and other soil-borne pathogens, and then lead to the occurrence of continuous cropping obstacles. Meloidogyne incognita (M.incognita), Java (M.javanica), peanut (M.arenaria) and northern (M.hapla) root-knot nematodes can all cause damage ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G2/30A01G22/05
CPCA01G2/30A01G22/05
Inventor 叶德友漆永红
Owner 甘肃省农业科学院蔬菜研究所
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