Preparation method and application of prunus mume flower bud total flavone extract
A technology of total flavonoids and extracts, which is applied in the field of medicine and achieves the effects of good tyrosinase activity, reduced melanin and less impurity content
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Embodiment 1
[0011] The preparation process and application of the total flavonoids extract of white plum blossoms specifically include the following steps: (1) Add white plum blossom medicinal materials according to the weight ratio of 1:10, add ethanol with a volume concentration of 50%, heat and reflux extraction twice, each time for 2 hours , the extracts were filtered and combined, concentrated under reduced pressure at 75°C to a clear ointment; (2) Take the clear ointment in step (1) and add 1 times the amount of water, cool down for 20 hours, and filter to obtain the sample drug solution (3) Sample drug The liquid was statically adsorbed by FL-1 macroporous adsorption resin, stirred for 10 minutes per hour, stirred 6 times in total, and stood overnight; adsorbed 3 times in total, in the desorption process, first eluted with 2BV of water, and discarded the eluent , and then carry out gradient elution with 4BV60% ethanol and 3BV80% ethanol solution, collect and combine the eluents, rec...
Embodiment 2
[0013] The preparation process and application of the total flavonoids extract of white plum blossoms specifically include the following steps: (1) adding ethanol with a volume concentration of 60% to the medicinal materials of white plum blossoms in a weight ratio of 1:12, heating and refluxing for 2 times, each time for 2 hours , the extracts were filtered and combined, and concentrated under reduced pressure at 75°C to a clear ointment; (2) Take the clear ointment in step (1) and add 0.7 times the amount of water, cool down for 20 hours, and filter to obtain the sample drug solution (3) Sample drug The liquid was statically adsorbed by FL-1 macroporous adsorption resin, stirred for 10 minutes per hour, stirred 6 times in total, and stood overnight; adsorbed 3 times in total, in the desorption process, first eluted with 2BV of water, and discarded the eluent , and then carry out gradient elution with 4BV60% ethanol and 3BV75% ethanol solution, collect and combine the eluents,...
Embodiment 3
[0015] The preparation process and application of the total flavonoids extract of white plum blossoms specifically include the following steps: (1) Add white plum blossom medicinal materials according to the weight ratio of 1:10, add ethanol with a volume concentration of 50%, heat and reflux extraction twice, each time for 2 hours , the extracts were filtered and combined, concentrated under reduced pressure at 75°C to a clear ointment; (2) Take the clear ointment in step (1) and add 1 times the amount of water, cool down for 20 hours, and filter to obtain the sample drug solution (3) Sample drug The liquid was statically adsorbed by D101 macroporous adsorption resin, stirred for 10 minutes per hour, stirred 6 times in total, and stood overnight; adsorbed 3 times in total, during the desorption process, first eluted with 2BV of water, discarded the eluent, and then Gradient elution is carried out with 4BV50% ethanol and 3BV870% ethanol solution, the eluents are collected, comb...
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