Injectable and degradable dry hemostatic cryogel with good shape memory and blood coagulation capability as well as preparation method and application of injectable and degradable dry hemostatic cryogel

Abstract

The invention relates to injectable and degradable dry hemostatic cryogel with good shape memory and blood coagulation capability as well as a preparation method and an application of the injectable and degradable dry hemostatic cryogel. Chitosan is resuspended in deionized water, glacial acetic acid is dropwise added under stirring, and a CS solution is obtained after uniform mixing; dopamine hydrochloride is added to the deionized water to prepare a DA solution; the CS solution is mixed with the DA solution, an oxidant solution is added simultaneously, and the solutions are uniformly mixed to obtain a mixed solution; and finally, the mixed solution is placed at the temperature of subzero 7-subzero 20 DEG C to react for 12-36 h, a cross-linked cryogel network in a frozen state is obtained, and is placed in the deionized water to be melted, freeze drying is conducted, and the injectable and degradable dry hemostatic cryogel with good shape memory and blood coagulation capability is obtained. The cryogel dressing has high elasticity and ultrafast shape recovery capability, can quickly absorb blood, and has photo-thermal antibacterial performance and the like.

Description

technical field

[0001] The invention belongs to the technical field of biomedical materials, and in particular relates to an injectable and degradable dry-state hemostatic gel with good shape memory and blood coagulation ability, and a preparation method and application thereof. Background technique

[0002] Hemostasis is an important issue that must be faced in war wounds and common wounds. More than 30% of traumatic deaths worldwide are caused by uncontrolled bleeding, and more than half of these deaths occur before the arrival of emergency care. Although many reported or commercial hemostatic agents have high haemostatic efficiency for superficial bleeding wounds, such as tissue adhesives, glutaraldehyde-crosslinked albumin, zeolite-based QuickClot, fibrin-based bandages, or gelatin-based hemostats, they are usually Ineffective against deep wounds caused by small caliber firearms, battlefield and IEDs in everyday life. Because these wounds are irregular in shape and can...

Examples

preparation example Construction

[0044] The preparation method of the present invention comprises the following steps:

[0045] 1) Resuspend chitosan (CS) in deionized water, then add glacial acetic acid dropwise with stirring, and then heat and stir at 50-60°C for 30-60 minutes to obtain CS solution; in CS solution, chitosan, deionized water The ratio between glacial acetic acid and glacial acetic acid is 1.0g:(39~160)mL:(1000~4000)μL; add dopamine hydrochloride (DA) into deionized water to make DA solution;

[0046] 2) After the CS solution, DA solution and oxidant solution are pre-cooled in an ice bath, mix the CS solution and DA solution, and at the same time add the oxidant solution and mix evenly to obtain a mixed solution, wherein the final mass concentration of CS is 0.5~ 2.0%, the final concentration of DA is 0.5-9 mg / mL; the oxidizing agent is sodium periodate; the molar ratio of sodium periodate to dopamine is 0.5-1:1;

[0047] 3) Put the mixed solution at -7~-20°C for 12~36h to obtain the frozen ...

Embodiment 1

[0052] Preparation of CS20 / PDA0.5 crystal gel: resuspend chitosan in deionized water, then add glacial acetic acid dropwise with stirring, and then heat and stir at 55°C for 40min to obtain 2.5wt% CS solution. In CS solution, chitosan The ratio between sugar, deionized water and glacial acetic acid is 1.0g: 39mL: 1000μL; add dopamine hydrochloride to deionized water to make a 5mg / mL DA solution; add sodium periodate to deionized water to make a 5.64 mg / mL SP solution; the molar ratio of sodium periodate to dopamine is 1:1. Subsequently, the CS solution, the DA solution and the SP solution were fully pre-cooled in an ice-water mixing bath and then fully mixed to obtain a final concentration of CS of 2.0 wt % and a final concentration of DA of 0.5 mg / mL. Then the mixture was placed in a low-temperature reactor at -20°C for 36 h. After the reaction, the crystal gel was melted in deionized water and freeze-dried at -80°C to obtain the injectable and degradable dry crystal gel hem...

Embodiment 2

[0054] The final concentration of DA in the step was controlled at 1.5 mg / mL, and other conditions were the same as in Example 1 to obtain CS20 / PDA1.5 crystal gel.

technical field

[0001] The invention belongs to the technical field of biomedical materials, and in particular relates to an injectable and degradable dry-state hemostatic gel with good shape memory and blood coagulation ability, and a preparation method and application thereof. Background technique

[0002] Hemostasis is an important issue that must be faced in war wounds and common wounds. More than 30% of traumatic deaths worldwide are caused by uncontrolled bleeding, and more than half of these deaths occur before the arrival of emergency care. Although many reported or commercial hemostatic agents have high haemostatic efficiency for superficial bleeding wounds, such as tissue adhesives, glutaraldehyde-crosslinked albumin, zeolite-based QuickClot, fibrin-based bandages, or gelatin-based hemostats, they are usually Ineffective against deep wounds caused by small caliber firearms, battlefield and IEDs in everyday life. Because these wounds are irregular in shape and can...

Examples

preparation example Construction

[0044] The preparation method of the present invention comprises the following steps:

[0045] 1) Resuspend chitosan (CS) in deionized water, then add glacial acetic acid dropwise with stirring, and then heat and stir at 50-60°C for 30-60 minutes to obtain CS solution; in CS solution, chitosan, deionized water The ratio between glacial acetic acid and glacial acetic acid is 1.0g:(39~160)mL:(1000~4000)μL; add dopamine hydrochloride (DA) into deionized water to make DA solution;

[0046] 2) After the CS solution, DA solution and oxidant solution are pre-cooled in an ice bath, mix the CS solution and DA solution, and at the same time add the oxidant solution and mix evenly to obtain a mixed solution, wherein the final mass concentration of CS is 0.5~ 2.0%, the final concentration of DA is 0.5-9 mg / mL; the oxidizing agent is sodium periodate; the molar ratio of sodium periodate to dopamine is 0.5-1:1;

[0047] 3) Put the mixed solution at -7~-20°C for 12~36h to obtain the frozen ...

Embodiment 1

[0052] Preparation of CS20 / PDA0.5 crystal gel: resuspend chitosan in deionized water, then add glacial acetic acid dropwise with stirring, and then heat and stir at 55°C for 40min to obtain 2.5wt% CS solution. In CS solution, chitosan The ratio between sugar, deionized water and glacial acetic acid is 1.0g: 39mL: 1000μL; add dopamine hydrochloride to deionized water to make a 5mg / mL DA solution; add sodium periodate to deionized water to make a 5.64 mg / mL SP solution; the molar ratio of sodium periodate to dopamine is 1:1. Subsequently, the CS solution, the DA solution and the SP solution were fully pre-cooled in an ice-water mixing bath and then fully mixed to obtain a final concentration of CS of 2.0 wt % and a final concentration of DA of 0.5 mg / mL. Then the mixture was placed in a low-temperature reactor at -20°C for 36 h. After the reaction, the crystal gel was melted in deionized water and freeze-dried at -80°C to obtain the injectable and degradable dry crystal gel hem...

Embodiment 2

[0054] The final concentration of DA in the step was controlled at 1.5 mg / mL, and other conditions were the same as in Example 1 to obtain CS20 / PDA1.5 crystal gel.

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