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Application of bile acid complex microbial inoculant in preparation of mutton sheep feed additive

A feed additive and compound bacterial agent technology, applied in the field of poultry breeding, can solve the problems of reduced ewe feeding, shortage of sheep source, lack of nutrition, etc. Effect

Active Publication Date: 2021-02-26
SHANDONG LONGCHANG ANIMAL HEALTH PROD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, most mutton sheep are fattened in different places, and most of them are animal husbandry and agricultural breeding. However, due to the prohibition of grazing and restricted grazing in pastoral areas, the number of ewes is reduced, resulting in a shortage of sheep resources. In addition, due to the lack of breeding standards, the feed ratio of some fattening farms is not good. Science will also lead to different sizes of mutton sheep, lack of nutrition, yellow fat disease due to nutritional problems, or increased fat content, which will affect the quality of mutton sheep and affect the breeding income of farmers, and now some veterinary drugs or other feeds are used Additives may have certain side effects and remain in the body of mutton. People will seriously endanger their health after eating

Method used

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  • Application of bile acid complex microbial inoculant in preparation of mutton sheep feed additive
  • Application of bile acid complex microbial inoculant in preparation of mutton sheep feed additive

Examples

Experimental program
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Effect test

Embodiment 1

[0025] 1. Screening and identification of Parabacteroides distirii LCG-06

[0026] 1. Screening of Parabacteroides distirii LCG-06

[0027] Take intestinal feces from the intestines of pigs, mix 10g of feces and 100mL sterile water, filter to remove impurities, and make a feces dilution, and then continue to add sterile water to the dilution to prepare 10 -1 、10 -2 、10 -3 、10 -4 、10 -5 diluent; then take 10 -2 、10 -3 、10 -4 、10 -5 Spread the dilution on soybean casein agar medium (TSA) in a medium containing 80% N 2 , 10% CO 2 and 10%H 2 Under an anaerobic environment, cultured at 37°C for 4 days, selected obvious colonies and purified them several times to obtain a single colony, which was named LCG-06, and then placed in soybean casein agar slant medium for refrigerated storage. The formula of the TSA medium is: tryptone 15.0g, soytone 5.0g, sodium chloride 5.0g, agar 15.0g, sterile water 1000mL, pH 7.3±0.2.

[0028] Such as figure 1 As shown, the biological cha...

Embodiment 2

[0048] In a farm in Shandong, 500 Boer goats were bred, of which 20 were randomly selected as the experimental group, 20 as the control group, and 40 goats were all 4-5 generation sheep. One month before the slaughter, the experimental group fed the goats with feed containing bile acid compound bacteria at noon every day, and mixed the bile acid compound bacteria into the feed at an amount of 30-50 mL per goat, and fed them continuously for 25 days , the sheep in the control group were fed the same amount of feed without adding bile acid compound bacteria agent. Concentrate feed and grass feed with the same mixing ratio were selected as feed, and all mutton sheep continued to be raised according to conventional breeding methods. Slaughtered after 25 days, the average slaughter weight of the mutton sheep in the control group was 56.44kg, and more yellow and white fat could be seen during slaughter; the average slaughter weight of the goats in the experimental group was 54.47 ca...

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Abstract

The invention discloses an application of a bile acid complex microbial inoculant in preparation of a mutton sheep feed additive. The bile acid complex microbial inoculant is prepared from a parabacteroides distasonis bacterial suspension and bile acid, wherein the parabacteroides distasonis bacterial suspension is obtained by culturing and fermenting parabacteroides distasonis LCG-06 with the preservation number of CGMCC No.20820. The bile acid complex microbial inoculant is prepared from natural components, free of toxic and side effects and capable of remarkably increasing the growth speedof mutton sheep, promoting nutrient absorption and accelerating decomposition of fat in the mutton sheep, then the fat percentage of the mutton sheep is reduced, the slaughter weight and the slaughterrate of the mutton sheep are increased, the breeding income of the mutton sheep is increased, and therefore, the bile acid complex microbial inoculant has wide application prospects.

Description

technical field [0001] The invention belongs to the field of poultry breeding, and in particular relates to the application of a bile acid compound bacterial agent in preparing mutton sheep feed additives. [0002] technical background [0003] Meat sheep is one of the most adaptable livestock to the external environment, and with the improvement of people's living standards in our country, the demand for mutton sheep is gradually increasing. At present, most mutton sheep are fattened in different places, and most of them are animal husbandry and agricultural breeding. However, due to the prohibition of grazing and restricted grazing in pastoral areas, the number of ewes is reduced, resulting in a shortage of sheep resources. In addition, due to the lack of breeding standards, the feed ratio of some fattening farms is not good. Science will also lead to different sizes of mutton sheep, lack of nutrition, yellow fat disease due to nutritional problems, or increased fat content...

Claims

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Application Information

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IPC IPC(8): A23K50/10A23K10/18A23K20/105C12N1/20C12R1/01
CPCA23K50/10A23K10/18A23K20/105C12N1/20C12R2001/01C12N1/205A61K31/575A23V2002/00A61K35/741A23V2200/3204A61K9/0056A61K39/0216A61K2035/115
Inventor 张西雷曹爱智娄倩倩籍立民
Owner SHANDONG LONGCHANG ANIMAL HEALTH PROD
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