Valeriana offcinalis L. total alkaloid extract and preparation method thereof

A technology of total alkaloids and extracts, applied in the field of valerian total alkaloids extract and preparation thereof, can solve problems such as no relevant literature reports on valerian total alkaloids, achieve high product yield, good application prospects, The effect of high pharmacodynamic activity

Active Publication Date: 2021-02-26
武汉惠尔生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, there is no relevant literature report on the extraction of total alkaloids of valerian

Method used

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  • Valeriana offcinalis L. total alkaloid extract and preparation method thereof
  • Valeriana offcinalis L. total alkaloid extract and preparation method thereof
  • Valeriana offcinalis L. total alkaloid extract and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Example 1 Extraction Technology Research

[0033] (1) Take valerian root, decoct and extract twice with 10 times the weight of water, each time for 1.5 hours, combine the two extracts, concentrate, and dry.

[0034] (2) Take valerian root, reflux extract with 10 times the weight of 40% ethanol (containing 0.3% hydrochloric acid) twice, each time for 1.5 hours, combine the two extracts, add sodium hydroxide to adjust the pH to 7.5, concentrate and dry.

[0035] (3) Take valerian root, reflux extract with 10 times the weight of 60% ethanol (containing 0.3% hydrochloric acid) twice, each time for 1.5 hours, combine the secondary extracts, add sodium hydroxide to adjust the pH to 7.5, concentrate, and dry.

[0036] (4) Take valerian root, reflux extract with 10 times the weight of 80% ethanol (containing 0.3% hydrochloric acid) twice, each time for 1.5 hours, combine the secondary extracts, add sodium hydroxide to adjust the pH to 7.5, concentrate, and dry.

[0037] The to...

Embodiment 2

[0043] Embodiment 2 extraction process research

[0044] Get valerian root, first use 10 times the weight of water to decoct and extract for 1.5 hours, filter, concentrate the extract into a thick ointment, and then use 10 times the weight of 60% ethanol (containing 0.3% hydrochloric acid) to reflux extract for 1.5 hours, filter, and Add sodium hydroxide to the extract to adjust the pH to 7.5, then concentrate until it has no alcohol smell, and then add it to the above thick paste and mix.

[0045] Add petroleum ether, chloroform, ethyl acetate, acetone, and n-butanol to the mixed thick paste for extraction, respectively obtain petroleum ether, chloroform, ethyl acetate, acetone, and n-butanol extraction parts, and recover the organic solvent And concentrate, dry, detect the total alkaloid content in the dry paste and measure the inhibition rate to Malassezia, the results are shown in Table 2.

[0046] Table 2 Comparison of total alkaloid content and Malassezia inhibition rat...

Embodiment 3

[0049] Embodiment 3 purification process research

[0050] In order to further improve the pharmacodynamic activity of the extract, the total alkaloids in the above-mentioned product are purified by a macroporous resin purification method, as follows:

[0051] 3.1 Screening of resin types

[0052] Resin types: D101, D201, AB-8, HP-10, XAD-2, ADS-8, HPD-100.

[0053] Resin pretreatment: Soak the resin in absolute ethanol for 24 hours, then rinse with clean water until there is no alcohol smell.

[0054] Resin regeneration: Soak the resin in 5% hydrochloric acid for 1-3 hours and rinse with pure water until neutral, then soak in 4% sodium hydroxide for 1-3 hours, then rinse with pure water until neutral.

[0055] (1) Take 10 g of the crude extract of the ethyl acetate part, dissolve it in water, and prepare a crude extract with a concentration of 10 mg / mL.

[0056] (2) Weigh 2g of each of the above 7 kinds of macroporous resins, add them into a 100mL conical flask, then add 5...

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Abstract

The invention discloses a preparation method of a valeriana offcinalis L. total alkaloid extract. The method comprises the following three steps: extracting valeriana offcinalis L. with water and / oran ethanol solution containing 0.1-0.5% hydrochloric acid, extracting the extract with an organic solvent, and finally purifying the extraction part with macroporous adsorption resin. The high-concentration total alkaloid extract can be separated and purified from the valeriana offcinalis L. medicinal material, and the prepared extract has very high pharmacodynamic activity.

Description

technical field [0001] The invention belongs to the field of pharmacy, and relates to a valerian total alkaloid extract and a preparation method thereof. Background technique [0002] Valerian (Valeriana offcinalis L.) is a plant of Patrine family, which is widely distributed all over the world, and its roots and rhizomes are mostly used as medicine. Valerian has antispasmodic, analgesic, sedative, and calming effects, and can play a certain therapeutic effect on bruises, restlessness, palpitations, insomnia, dysmenorrhea, amenorrhea, and rheumatic arthralgia. Valerian root mainly contains active ingredients of volatile oil, iridoids, sesquiterpenes, alkaloids, lignans, and flavonoids, and is an excellent natural drug resource. At present, there are not many research reports on the chemical constituents and activities of valerian, mainly focusing on volatile oils, iridoids, sesquiterpenes, and lignans, while there are very few studies on alkaloids. According to individual ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/84A61P17/00A61P31/10
CPCA61K36/84A61P17/00A61P31/10A61K2236/333A61K2236/55
Inventor 熊义涛蔡凯曾庆奇王红芳
Owner 武汉惠尔生物科技有限公司
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