Raman detection kit and Raman detection method for organophosphorus

A detection kit and detection method technology, applied in Raman scattering, measurement devices, instruments, etc., can solve the problems of low accuracy, low detection sensitivity, long time consumption, etc., and achieve low cost, simple components, and simple preparation. Effect

Active Publication Date: 2021-02-26
XIAMEN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the above methods generally have problems such as long time-consuming, low accuracy, and low detection sensitivity.

Method used

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  • Raman detection kit and Raman detection method for organophosphorus
  • Raman detection kit and Raman detection method for organophosphorus
  • Raman detection kit and Raman detection method for organophosphorus

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] In this example, the preparation of the Raman detection kit for organophosphorus and the Raman detection of the analytes were carried out at room temperature.

[0040] Prepare the sample to be tested: prepare a solution with a concentration of 1000mg / LDMMP as the sample to be tested.

[0041] For the preparation of Au sol, add chloroauric acid solution into 50mL water, heat to boil, then add sodium citrate solution, continue heating for 30min, and cool to room temperature, wherein the molar ratio of chloroauric acid to sodium citrate is (4-6): 3. Obtain Au sol with a particle size of 30-100nm.

[0042] For the preparation of core-shell nanoparticle sol, disperse the obtained Au sol in 50mL ultrapure water, add about 3mL of sodium citrate with a mass fraction of 1% and ascorbic acid with a mass fraction of 1%, mix well and then add 10mL of concentration dropwise 20mmol / L silver nitrate solution, stirred for a certain period of time, centrifuged, washed, and concentrated...

Embodiment 2

[0047] In this example, the preparation of the Raman detection kit for organophosphorus and the Raman detection of the analytes were carried out at room temperature.

[0048] Prepare the sample to be tested: prepare DMMP mother solution with a concentration of 1000mg / L, and dilute them respectively to obtain DMMP standard solutions with a concentration of 100mg / L, 10mg / L, 1mg / L, 100μg / L, 50μg / L and 10μg / L as the sample to be tested .

[0049] For the preparation of Au sol, take 1% chloroauric acid solution and add it to 50mL water, heat to boil, then add 2mL sodium citrate solution with a concentration of 1%, continue heating for 30min, and cool to room temperature to obtain a particle size of 30 - 100nm Au sol.

[0050] For the preparation of core-shell nanoparticle sol, disperse the obtained Au sol in 50mL ultrapure water, add about 3mL of sodium citrate with a mass fraction of 1% and ascorbic acid with a mass fraction of 1%, mix well and then add 10mL of concentration drop...

Embodiment 3

[0055] Prepare the sample to be tested: prepare a DMMP mother solution with a concentration of 1000 mg / L, and dilute it to obtain a DMMP standard solution with a concentration of 100 mg / L as the sample to be tested.

[0056] In Example 3, the same process conditions as in Example 2 of the present invention were used to detect the 100 mg / L DMMP sample to be tested. The difference was that Example 3 was carried out at 10°C and 50°C respectively.

[0057] Figure 5 is the Raman detection spectrum of the solution to be tested under different temperature conditions. The results show that the organophosphorus Raman detection kit and the Raman detection method of the present invention can obtain relatively stable and strong signals in the face of large fluctuations in the temperature of the test environment.

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Abstract

The invention provides a Raman detection kit for organophosphorus. The detection kit is characterized by comprising an SERS enhanced chip and an enhancement aid, wherein the SERS enhanced chip comprises a substrate and core-shell structure nanoparticles, and the core-shell structure nanoparticles are attached to the substrate, and the enhancement aid is KI. The invention also provides a Raman detection method using the organophosphorus detection kit. According to the invention, the SERS enhanced chip is prepared by coating the surface of the substrate with the core-shell structure nanoparticles as an enhanced matrix, and a certain amount of an enhancing aid is added into a to-be-detected sample, so that the detection sensitivity of the organophosphorus toxic agent is improved, the concentration of organophosphorus can be detected to be as low as 10 micrograms per liter, and the limitation of low sensitivity of a portable Raman spectrometer is broken through; in addition, the Raman detection kit and the Raman detection method provided by the invention can obtain relatively stable and relatively strong signals when facing relatively large test environment temperature fluctuation.

Description

Technical field: [0001] The invention relates to the field of rapid Raman detection, in particular to a Raman detection kit and a Raman detection method for organophosphorus. Background technique: [0002] Organophosphorus poisons include organophosphorus pesticides and nerve agents (NAs), both of which belong to organophosphorus compounds. Among them, nerve agents are the most toxic chemical warfare agents, and have been used many times in wars or terrorist attacks. Organophosphorus poisons include sarin (GB), soman (GD), tabun (GA), Viex (VX), malathion, phoxim, methyl parathion, paraoxon, etc. Wherein chemical warfare agent sarin (isopropyl meflodronate, GB) is a kind of commonly used military nerve agent, can destroy the function of nervous system by inhibiting acetylcholinesterase, and the degradation rate in human body is very slow, has cumulative toxicity. Therefore, in recent years, chemical defense and other departments urgently need a high-sensitivity, portable ...

Claims

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Application Information

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IPC IPC(8): G01N21/65
CPCG01N21/658
Inventor 李剑锋温宝英
Owner XIAMEN UNIV
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