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Analysis method of aristolochic acid and/or aristololactam

An aristolochid lactam and analysis method technology, which is applied in the fields of mass spectrometry analysis, Chinese herbal medicine analysis, and analytical chemistry, can solve the problems of inability to achieve quantitative analysis, difficult quantification, long analysis time, etc., achieve qualitative and quantitative analysis, reduce batch The effect of improving the analysis efficiency

Active Publication Date: 2021-03-02
SHIMADZU (CHINA) CO LTD
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

For the combination of LC and high-resolution mass spectrometry, it is mainly used in the qualitative analysis of aristolochic acids, and it is difficult to perform accurate quantification
[0005] As can be seen above, in the prior art, in the quantitative analysis of aristolochic acids by mass spectrometry, the LC separation step must be performed in advance, and the analysis time is very long. On the other hand, based on high-resolution mass spectrometry, although it can achieve rapid Qualitative analysis, but unable to achieve reliable quantitative analysis

Method used

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  • Analysis method of aristolochic acid and/or aristololactam
  • Analysis method of aristolochic acid and/or aristololactam

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Embodiment

[0126] Examples of the present invention will be described below, but the present invention is not limited to the following examples.

[0127] In this example, a probe electrospray ion source is used in series with a triple quadrupole mass spectrometer to analyze aristolochic acid A, aristolochic acid B, aristolochic acid C, and aristolochic acid D in Chinese herbal medicines Tianxianteng and Asarum , Aristolocholactam I.

[0128] The configuration of the device and the conditions, steps and results of the detection are described in detail below.

[0129] In the present example, according to the following steps, the Caulis radix and Asarum as samples were respectively crushed, extracted with methanol water, and after dilution, they were supplied to the probe electrospray ion source series triple quadrupole mass spectrometer, and the internal standard method was used to for analysis.

[0130] (1) Get a 100g sample, crush it with a pulverizer, and pass a 0.35mm sample sieve; ...

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Abstract

The present invention relates to an analysis method of aristolochic acid and / or aristololactam. According to the invention, for a plant-derived sample, a probe electrospray ionization mass spectrometry is used; the analysis method of aristolochic acid and / or aristololactam comprises a dilution step for adding a buffer solution to the sample and diluting the sample; and a mass spectrometry step inwhich the diluted sample is subjected to probe electrospray ionization and introduced into a mass spectrometry device, wherein the buffer solution contains an ammonium salt and carboxylic acid havingan acid dissociation constant pKa below 4.8.

Description

technical field [0001] The invention relates to the fields of analytical chemistry, mass spectrometry, and Chinese herbal medicine analysis, and in particular to an analysis method for aristolochic acid and / or aristolochic lactam. Background technique [0002] Aristolochic acid is a large class of nitrophenanthrene compounds, mainly aristolochic acid A, B, C, D (also sometimes called aristolochic acid I, II, III, IV) and so on. Aristolochic acid is contained in many Aristolochiaceae plants such as Aristolochia and Asarum, and often coexists with aristolochic lactam. These plants are widely used as Chinese herbal medicines, such as Tianxianteng, Aristolochia, Xugufeng, Zhushalian, Asarum, etc. On October 27, 2017, in the list of carcinogens released by the International Agency for Research on Cancer of the World Health Organization, aristolochic acid and plants containing aristolochic acid are included in the list of carcinogens. In addition, aristolochic acid can induce re...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/62G01N1/28G01N1/38H01J49/16
CPCG01N27/62G01N1/286G01N1/38H01J49/167G01N2001/386
Inventor 陈振贺滨田尚树
Owner SHIMADZU (CHINA) CO LTD
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