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Application of cordyceps sinensis extract in preparation of angiogenesis promoting medicines and/or medicines for treating ischemic cardiovascular and cerebrovascular diseases

A technology for cardiovascular and cerebrovascular diseases and Cordyceps sinensis, which is applied in the field of medicine and achieves the effects of low cost, excellent application prospect and promotion of pharmacodynamic activity.

Inactive Publication Date: 2021-03-05
INST OF ZOOLOGY GUANGDONG ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The use of Cordyceps sinensis as an angiogenesis drug has not been reported yet

Method used

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  • Application of cordyceps sinensis extract in preparation of angiogenesis promoting medicines and/or medicines for treating ischemic cardiovascular and cerebrovascular diseases
  • Application of cordyceps sinensis extract in preparation of angiogenesis promoting medicines and/or medicines for treating ischemic cardiovascular and cerebrovascular diseases
  • Application of cordyceps sinensis extract in preparation of angiogenesis promoting medicines and/or medicines for treating ischemic cardiovascular and cerebrovascular diseases

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] The preparation of embodiment 1 Cordyceps sinensis extract of the present invention

[0030] 1. Take 1kg of Cordyceps sinensis powder, add 30 times the amount of ethyl acetate (30L) to soak at room temperature for 9 hours, after soaking, ultrasonic extraction for 30 minutes, ultrasonic power 250w, filter, repeat soaking and extraction 3 times, combine the filtrate, concentrate into an extract, Obtain ethyl acetate extract;

[0031] 2. After the powder residue of Cordyceps sinensis is dried, add 30 times the amount of ethanol aqueous solution (30L) with a volume fraction of 95%, heat and reflux at 90°C for 30 minutes, filter, repeat the extraction 3 times, combine the filtrates, concentrate into an extract, and obtain ethanol extraction thing;

[0032] 3. After the Cordyceps sinensis residue is dried, add 30 times the amount of pure water (30L), heat and reflux at 100°C for 60 minutes, filter, repeat the extraction 3 times, combine the filtrates, concentrate into an ext...

Embodiment 2

[0034] Example 2 Angiogenesis-promoting Experiment of Cordyceps Sinensis Extract under Normal Oxygen Concentration Conditions

[0035] Experimental purpose: Under normal oxygen concentration, the angiogenesis-promoting activity of the Cordyceps sinensis extract in Example 1 was determined using the chicken embryo chorioallantoic membrane model.

[0036] Experimental grouping: set up negative control group (DMSO solvent), positive control group (recombinant human VEGF165), Cordyceps sinensis extract group (25mg crude drug amount / mL, 35mg crude drug amount / mL, 50mg crude drug amount / mL), 25 chickens in each group Embryo.

[0037] experimental method:

[0038] Chicken embryo hatching: Chicken embryo hatching is carried out in an artificial climate chamber, with a set temperature of 37.5°C and a humidity of 50%, for continuous incubation for 7 days, and flipping 2 to 4 times a day to make the chicken embryo develop and produce blood vessels.

[0039] Open the window of the chicken...

Embodiment 3

[0049] Example 3 Cordyceps sinensis extract promotes angiogenesis experiment under low hypoxic conditions

[0050] Experimental purpose: Under low hypoxic conditions, the angiogenesis-promoting activity of the Cordyceps sinensis extract in Example 1 was determined using a chicken embryo chorioallantoic membrane model.

[0051] Experimental grouping: set up negative control group (DMSO solvent), positive control group (recombinant human VEGF165), Cordyceps sinensis extract group (25mg crude drug amount / mL, 35mg crude drug amount / mL, 50mg crude drug amount / mL), 25 chickens in each group Embryo.

[0052] Modeling under low hypoxic conditions: Add 2 packs of microaerophilic air-generating bag tranquilizer agent C-02 in a sealed bag of 60*70cm, and replace the tranquilizer every 24 hours to keep the oxygen concentration lower than the normal oxygen concentration. The oxygen concentration was between 5% and 12%. Chicken embryos were cultured in this environment to investigate the e...

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Abstract

The invention discloses an application of cordyceps sinensis extract in preparation of angiogenesis promoting medicines and / or medicines for treating ischemic cardiovascular and cerebrovascular diseases. According to the cordyceps sinensis extract, cordyceps sinensis is used as a raw material, ethyl acetate, an ethanol aqueous solution and water are sequentially used for extraction, an ethyl acetate extract, an ethanol extract and a water extract are obtained respectively, the ethyl acetate extract, the ethanol extract and the water extract are mixed evenly and then dried, and the cordyceps sinensis extract is obtained. Experiments prove that the cordyceps sinensis extract provided by the invention has remarkable pharmacodynamic activity of promoting angiogenesis under the conditions of normal oxygen concentration and hypoxia. The cordyceps sinensis extract disclosed by the invention has the potential of developing the angiogenesis promoting medicines, is used for treating the ischemiccardiovascular and cerebrovascular diseases such as coronary heart disease, myocardial infarction and cerebral infarction, and has an excellent application prospect. The preparation method of the cordyceps sinensis extract is simple, low in cost, safe, non-toxic and suitable for industrial production.

Description

technical field [0001] The invention belongs to the technical field of medicine, and specifically relates to the application of Cordyceps sinensis extract in the preparation of drugs for promoting angiogenesis and / or drugs for treating ischemic cardiovascular and cerebrovascular diseases. Background technique [0002] Cardiovascular and cerebrovascular diseases are collectively referred to as cardiovascular and cerebrovascular diseases. Disease is a common disease that seriously threatens human health. It has the characteristics of high prevalence, high disability and high mortality. The number of people who die of cardiovascular and cerebrovascular diseases in the world is as high as 15 million every year, ranking first among all causes of death. first place. [0003] Ischemic stroke (IS), also known as cerebral infarction, is a common type of cerebrovascular disease and belongs to the category of "stroke" in traditional Chinese medicine. IS is one of the major diseases c...

Claims

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Application Information

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IPC IPC(8): A61K36/068A61P9/10
CPCA61K36/068A61K2236/333A61K2236/35A61K2236/39A61P9/10
Inventor 龙海林饶中臣吴华刘桂清曹莉韩日畴
Owner INST OF ZOOLOGY GUANGDONG ACAD OF SCI
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