High-yield separation and purification method of prostaglandin E2

A prostaglandin, separation and purification technology, applied in the field of high-yield separation and purification of prostaglandin E2, can solve the problems of failing to meet qualified standards, low sample purity, etc., and achieve the effects of short purification cycle and no pollution cost

Active Publication Date: 2021-03-12
SUZHOU NANOMICRO TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

CN111394289A is mainly a genetic engineering production method, and the purity of the obtained sample is extremely low, only 2.547%, which is far from reaching the qualified standard

Method used

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  • High-yield separation and purification method of prostaglandin E2
  • High-yield separation and purification method of prostaglandin E2
  • High-yield separation and purification method of prostaglandin E2

Examples

Experimental program
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Effect test

Embodiment 1

[0026] A sample was taken, and the crude prostaglandin E2 was dissolved in an acidic solution with a content of 2 mg / mL. After the solution was clarified, it was filtered through a filter membrane with a pore size of 0.45 μm, and the filtrate was collected for later use. A chromatographic column of 4.6×250 mm was used, and UniSil C18 or C8 microspheres (manufactured by Suzhou Nawei Technology Co., Ltd.) were used as the packing material of the chromatographic column, and the packing volume was 4.2 ml. Pre-column pretreatment was performed on the chromatographic column, and 0.2% acetic acid aqueous solution (phase A) and acetonitrile (phase B) were used as mobile phases for gradient elution, and the flow rate was controlled at 0.7ml / min. The isocratic elution process is as follows: wash with 30% phase B for 72 minutes, collect the solution of the target peak in sections, summarize the components that meet the requirements, and analyze by high performance liquid chromatography. ...

Embodiment 2

[0029] Take a sample, and the crude prostaglandin E2 is dissolved in an acidic aqueous solution with a content of 2 mg / mL. After the solution was clarified, it was filtered with a filter membrane with a pore size of 0.45 μm, and the filtrate was collected for later use. A chromatographic column of DAC50×250mm was used, and UniSilC18 or C8 microspheres (produced by Suzhou Nawei Technology Co., Ltd.) were used as the chromatographic column filler, and the column volume was 490.6ml. Pre-column pretreatment was performed on the chromatographic column, and 0.2% acetic acid aqueous solution (phase A) and acetonitrile (phase B) were used as mobile phases for gradient elution, and the flow rate was controlled at 50 ml / min. The isocratic elution process is as follows: wash with phase B with a content of 30% for 80 minutes, collect the solution of the target peak in sections, summarize the components that meet the requirements, and analyze by high performance liquid chromatography. The ...

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Abstract

The invention provides a high-yield separation and purification method of prostaglandin E2. The method comprises the following steps: dissolving and filtering a prostaglandin crude product; loading the filtered solution into a chromatographic column filled with homogeneous reversed-phase silica gel for chromatography; eluting the target product by adopting an acetic acid aqueous solution and an organic solvent as mobile phases; and collecting solutions with target peak values in sections, and summarizing the component solutions meeting the requirements. Monodisperse UniSil C18 or UniSil C8 isadopted as a stationary phase, the separation method is simple, large-scale production can be achieved, and the recovery rate is high and stable.

Description

technical field [0001] The invention relates to the field of medicine purification, in particular to a method for separating and purifying prostaglandin E2 with high yield. Background technique [0002] Prostaglandin E2, also known as dinoprostone, the English name is abbreviated as PGE2, prostaglandin E2 (PGE2) is an important cell growth and regulatory factor, is arachidonic acid cyclooxygenase metabolites, is eicosan unsaturated fatty acid. PGE2 can cause strong uterine contraction, affect placental blood supply and placental function, and cause miscarriage. The mechanism of contraction of uterine smooth muscle may be related to the increase of free calcium release in uterine smooth muscle cells by prostaglandins. PGE2 is different from oxytocin in that it has excitatory effects on the uterus of all stages of pregnancy, and it is relatively mild, and its uterine contraction effect is 10 to 40 times stronger than that of PGE2α. PGE2 can transform and dilate the cervix, ...

Claims

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Application Information

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IPC IPC(8): C07C405/00
CPCC07C405/00C07C2601/08
Inventor 王希邓杰李建文
Owner SUZHOU NANOMICRO TECH CO LTD
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