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Silver nano hydrogel for regulating flora to activate immune response and preparation method and application thereof

An immune response and hydrogel technology, applied in the field of biomedicine, can solve the problems of inability to specifically regulate the commensal flora, hinder the effect of immunotherapy, and low responsiveness of malignant tumors, so as to improve host immunity, low equipment requirements, Addressing the effect of low tumor response rates

Active Publication Date: 2021-03-16
WUHAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] Under the background of low responsiveness of malignant tumors and impeding the effect of immunotherapy, the purpose of the present invention is to provide a gel product that can be applied to the prevention or treatment of tumors in view of the defects that the current gel products have a single function and cannot specifically regulate the commensal flora. Silver nano hydrogel for regulating bacterial flora to activate immune response and preparation method thereof

Method used

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  • Silver nano hydrogel for regulating flora to activate immune response and preparation method and application thereof
  • Silver nano hydrogel for regulating flora to activate immune response and preparation method and application thereof
  • Silver nano hydrogel for regulating flora to activate immune response and preparation method and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0029] Example 1 Preparation method of silver nanohydrogel for regulating flora to activate immune response

[0030] AgNO 3 and dextran are dissolved in deionized water at a ratio of 1:10-5:1 to form a 0.2-1wt% solution. Silver nanoparticles are obtained by centrifugation after UV irradiation at room temperature, and dispersed in 0.5-2wt% chitosan solution to obtain solution A. The concentration of silver nanoparticles in solution A is 0.2-1.2 mg / mL. Dissolve dextran in deionized water to form a 1-5wt% solution, and add KIO with a mass ratio of 1:5-1:10 to dextran 4 , after stirring for 12 hours, a solution B containing 1-5 wt% polyaldehyde dextran was formed. Such as figure 2 As shown, the silver nanohydrogel can be obtained by mixing solution A and solution B at a volume ratio of 1:2 to 3:1.

Embodiment 2

[0031] Example 2 Retention effect of silver nano hydrogel affected area by regulating flora to activate immune response

[0032] The 6-week-old female C57BL / 6 mice were fed with drinking water containing 100 μg / mL 4-NQO for 16 weeks to establish an orthotopic mouse model of oral squamous cell carcinoma.

[0033] 10 mg of cyanine dye Cy5 and 1 g of chitosan were prepared into a 1 wt % Cy5-chitosan solution and stirred overnight. Add 0.68g AgNO to 150mL deionized water3 And 0.35g dextran T100, UV irradiation at room temperature, centrifugation at 5000g after stirring for 12h to obtain AgNP, dispersed in 1wt% Cy5-chitosan solution to obtain solution A, adjust the concentration of silver nanoparticles in solution A to 0.6mg / mL. Dissolve 1 g of Dextran T100 in 50 mL of deionized water, add 0.1 g of KIO 4 , solution B was formed after stirring for 12 h. Mix solution A and solution B at a volume ratio of 1:2 to obtain a silver nanohydrogel labeled with cyanine dye Cy5.

[0034] Th...

Embodiment 3

[0035] Example 3 Adjusting the Effect of the Silver Nanohydrogel on Regulating the Bacteria to Activate the Immune Response

[0036] Add 0.57g AgNO to 150mL deionized water 3 and 0.25g dextran T100, UV irradiation at room temperature, stirring reaction for 12h, and centrifugation at 5000g to obtain AgNPs.

[0037] Add 10 g of urea to 5 mL of deionized water, and place the suspension in an alumina crucible. The mixture was heated to 550 °C, reacted for 3 hours, cooled to 25 °C, washed three times with deionized water to obtain C 3 N 4 nanoparticles.

[0038] 13g FeCl 3 ·6H 2 O and 6.5 g FeCl 2 4H 2 O was dissolved in 80 mL of deionized water, and nitrogen gas was injected into the above solution for 0.5 h. Add 45 mL of NH dropwise at 80°C 3 ·H 2 O, then 7.5 g of citric acid dissolved in 15 mL of deionized water was added. After reacting at 95°C for 90 minutes, the obtained Fe 3 o 4 Nanoparticles were purified using dialysis bags (MWCO: 14000 Da).

[0039] Cellulos...

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Abstract

The invention discloses silver nano hydrogel for regulating flora to activate immune response and a preparation method and application thereof, and belongs to the field of biological medicine. The silver nano hydrogel consists of silver nanoparticles and mucous membrane adhesion gel. The silver nano hydrogel contains inorganic silver nanoparticles, natural high-molecular polysaccharide, a polyaldehyde polymer material and a deacetylation polymer material. The silver nano hydrogel can adjust flora abundance of mucous membranes, can inhibit proliferation of other bacteria, and meanwhile, can promote proliferation of immunologically activated bacteria such as anaerobic streptococcus and the like, so that immune response is enhanced. Meanwhile, the gel can also be adhered to human mucous membranes, so that long-time retention at affected parts is implemented, and the human symbiotic flora is continuously regulated. The silver nano hydrogel is biodegradable, excellent in biocompatibility, safe and free of toxic and side effects, and has the advantages of simplicity and convenience for preparation, continuity, high efficiency and the like. The silver nano hydrogel has important social and economic significance in the field of biomedicine of regulating the symbiotic flora, activating human immunity, assisting cancer resistance and the like.

Description

technical field [0001] The invention belongs to the field of biomedicine, and in particular relates to a silver nano hydrogel for regulating bacterial flora to activate an immune response, a preparation method and application thereof. Background technique [0002] Immunotherapy is a treatment modality that works by mobilizing the body's immune system to eliminate cancer. This method is of great significance for the treatment of high antigen-loaded tumors, but the low response rate has become the main obstacle to limit the efficacy of immunotherapy. Up-regulating the response rate of immunotherapy is one of the important breakthroughs to improve the effect of immunotherapy. [0003] There is evidence that the spatial location of the microbiota and the pathogenesis of certain cancers coincides highly and is closely linked to the effectority of the host immune system. Bacteria with immune activation effect can effectively up-regulate the intensity of host immune response. It ...

Claims

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Application Information

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IPC IPC(8): A61K33/38A61K9/06A61K47/36A61K47/38A61P35/00A61P37/04
CPCA61K33/38A61K9/06A61K9/0014A61K47/36A61K47/38A61P35/00A61P37/04
Inventor 张先正郑迪威严雨曾旋
Owner WUHAN UNIV
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