Injectable protein hydrogel stent and application thereof in prolonging in-vivo stability of drug
A hydrogel and protein technology, applied in the application field of injectable protein hydrogel scaffolds and prolonging the stability of drugs in vivo, can solve the problems of unfavorable gel fixed-point molding tolerance, poor biocompatibility and biodegradability and other problems, to achieve good volume support effect, prolong the stability in the body, and fast coagulation speed
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Embodiment 1
[0043] Effects of hydrogel decomposition on the body:
[0044] Preparation process of injectable protein hydrogel:
[0045] Accurately weigh 16mg of Fmoc-Phe and 12mg of Phe2 and slowly add it to the mixed solution of 1mL PBS (pH 7.4) and 420μL of 0.5mol / L NaOH and stir slowly until it is completely dissolved. At this time, the final concentration of the solution is Fmoc-Phe11.3mg / mL and Phe2 8.5mg / mL. Adjust the pH to 7.0 with 0.1 mol / L HCl solution, and the volume of the solution is about 3.5 mL. Pseudomonas fluorescens lipase (PFL) was completely dissolved in PBS (pH 7.4) to prepare 100 μL of a 50 mg / mL PFL solution. Slowly add the prepared PFL solution into the amino acid hydrogel precursor solution and mix well.
[0046] The prepared hydrogel sample was transferred to a quartz glass slide, and the sample was fully dehydrated and dried using a vacuum dryer, then ground, and the Fourier transform infrared (FTIR) spectrum of the hydrogel dry powder was measured and record...
Embodiment 2
[0049] The light transmittance of hydrogel with a thickness of 1cm at different wavelengths:
[0050] Preparation process of injectable protein hydrogel:
[0051] The amino acid hydrogel sample is placed in the detection cell, and the UV spectrum is recorded with an ultraviolet-visible spectrophotometer, and the wavelength range of the recorded spectrum is from 200nm to 1000nm. The light transmittance of the current wavelength can be calculated from the absorbance value corresponding to the wavelength.
[0052] Such as figure 2 Shown is the transmittance at different wavelengths of the hydrogel with a thickness of 1cm. It can be seen from the figure that at the ICG absorption wavelength peak at 808nm, the transmittance of the amino acid hydrogel with a thickness of 1cm (light path of the cuvette) is about 84.5 %, has higher light transmission ability.
Embodiment 3
[0054] Injectable hydrogel scaffold entrapped red blood cell-insulin:
[0055] Preparation process of erythrocyte-insulin hydrogel:
[0056] Accurately weigh 16mg of Fmoc-Phe and 12mg of Phe2 and slowly add it to the mixed solution of 1mL PBS (pH 7.4) and 420μL of 0.5mol / L NaOH and stir slowly until it is completely dissolved. At this time, the final concentration of the solution is Fmoc-Phe11.3mg / mL and Phe2 8.5mg / mL. Adjust the pH to 7.0 with 0.1 mol / L HCl solution, and the volume of the solution is about 3.5 mL.
[0057] Pseudomonas fluorescens lipase (PFL) was completely dissolved in PBS (pH 7.4) to prepare 100 μL of 50 mg / mL PFL solution. Slowly add the prepared PFL solution into the amino acid hydrogel precursor solution and mix well.
[0058] Take 1 mL of insulin-loaded red blood cells and add amino acid hydrogel precursor solution in an appropriate volume ratio, then add 100 μl of 50 mg / mL PFL solution, mix slowly, and place in a constant temperature water bath at 3...
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