Unlock instant, AI-driven research and patent intelligence for your innovation.

Streptomyces badius and application thereof

A technology of Streptomyces chestnutii and fermented products, applied in Streptomyces chestnutus and its application fields, can solve the problem of high investment and achieve the effect of high resistance to root nematodes and high lethality

Active Publication Date: 2021-03-19
INST OF MICROBIOLOGY - CHINESE ACAD OF SCI
View PDF3 Cites 1 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] In recent years, root-knot nematodes have become a serious problem for growers. When dealing with root-knot nematodes, not only the investment is high, but it is also difficult to

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Streptomyces badius and application thereof
  • Streptomyces badius and application thereof
  • Streptomyces badius and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0053] Embodiment 1, isolation, identification and preservation of bacterial strains

[0054]1. Collection of strain MS170035

[0055] In September 2017, a strain was isolated from sea mud in the South China Sea, named strain MS170035.

[0056] 2. Identification of strain MS170035

[0057] Morphological characteristics: colonies are raised, mycelia in the base are dark brown, a small amount of white aerial hyphae, spores are white, and no water-soluble pigment is produced.

[0058] The partial sequence of the 16S rRNA gene is shown in the sequence 1 of the sequence list, and has the highest sequence similarity with GENBANK ACCESSION NO.MK156399.1, with a similarity of 99.79%.

[0059] According to the identification results, the strain MS170035 belongs to Streptomyces badius.

[0060] 3. Preservation of strain MS170035

[0061] Streptomyces badius MS170035 was deposited in the General Microorganism Center of China Committee for Culture Collection of Microorganisms (CGMCC f...

Embodiment 2

[0062] Example 2, Preparation of the crude extract of Streptomyces chestnutus MS170035 and detection of its inhibitory effect on root-knot nematodes 1. Preparation of the crude extract of Streptomyces chestnutus MS170035

[0063] 1. Streptomyces chestnutus MS170035 was streak-inoculated on the slant medium, and cultured at 28° C. for 5 days (to make the aerial hyphae grow plumply) to obtain the slant strain.

[0064] Preparation method of slant medium: dissolve 4 grams of yeast extract, 10 grams of malt extract, 4 grams of glucose and 20 grams of agar powder in water, adjust the pH to 7.0-7.2, dilute to 1L with water, and extinguish at 115 °C Bacteria for 30 minutes.

[0065] 2. Divide the seed culture medium into 500ml Erlenmeyer flasks (100ml / bottle), inoculate the slanted bacterial species into the seed culture medium, shake and culture at 28°C and 200rpm for 5 days to obtain OD 600nm = 1.2-1.4 seed liquid.

[0066] The preparation method of the seed medium: dissolve 4 gr...

Embodiment 3

[0080] Embodiment 3, prepare compound and detect its inhibitory action to root-knot nematode

[0081] 1. Preparation of compounds

[0082] 1. The crude extract prepared in Step 1 of Example 2 was redissolved with methanol, filtered to remove insoluble matter, and then reversed high performance liquid chromatography was performed.

[0083] Chromatographic column: Agilent Eclipse SB C-18 reverse phase chromatographic column (9.4×250mm).

[0084] Detection wavelength: 254nm.

[0085] Mobile phase: 65% methanol aqueous solution (that is, the volume percentage of methanol is 65%).

[0086] Mobile phase flow rate: 3ml / min.

[0087] Collect the post-column solution of the target peak (the target peak is the fifth elution peak, and the retention time corresponding to the peak is 11.7 min). Chromatogram see figure 1 , the arrow marks the target peak.

[0088] 2. Take the post-column solution obtained in step 1, and then use a rotary evaporator to distill under reduced pressure at...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
wavelengthaaaaaaaaaa
Login to View More

Abstract

The invention discloses streptomyces badius and application thereof. The preservation registration number of the streptomyces badius MS170035 is CGMCC No. 21280. The invention further provides a method for preparing streptomyces badius extract. The method comprises the following steps of fermenting the streptomyces badius MS170035 to obtain a fermentation product; taking supernatant of the fermentation product, extracting by adopting an organic reagent, and collecting an extracting solution; and subjecting the extracting solution to reduced pressure distillation to remove an organic solvent toobtain the extract. The invention further protects the application of the streptomyces badius MS170035 or the extract, namely the application in preparing a nematode inhibitor and in nematode inhibition. The fermentation extract of the streptomyces badius provided by the invention has very high resistance to root nematode activity, compound separation is carried out on the fermentation extract ofthe streptomyces badius to obtain a compound represented by formula (I), and the compound has a high fatality rate on root nematode.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a strain of Streptomyces chestnutus and application thereof. Background technique [0002] In recent years, root-knot nematode has become a serious problem for growers. When dealing with root-knot nematode, not only the investment is high, but it is also difficult. Root-knot nematode is a worldwide pest. Generally, affected greenhouses can reduce production by 20%-30%. In severe greenhouses, the production reduction can reach 60%, or even extinction. The degree of damage is quite serious, and once introduced, it is difficult to eradicate. [0003] The insects are small and concealed: nematodes are a kind of soil-borne insect pests. They are small in size and strong in invading ability. [0004] Damage to the root, difficult to find: When the plant shows symptoms, the root has been invaded by nematodes, which has caused damage to the plant. The plant performance is simila...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12P17/18A01N63/28A01P5/00C07D495/04A01N43/90C12R1/465
CPCC12N1/20C12P17/187A01N63/28C07D495/04A01N43/90
Inventor 宋福行杨娜王龙
Owner INST OF MICROBIOLOGY - CHINESE ACAD OF SCI