Plant bactericide and application thereof
A plant fungicide, crop technology, applied in the directions of fungicide, plant growth regulator, application, etc., can solve the problem of less glycyrrhizic acid and other problems, and achieve the effect of no toxic side effects and difficult drug resistance.
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Embodiment 1
[0030] On the ultra-clean workbench, firstly inoculate PDA medium with Peronospora lychee, Phytophthora sojae, Fusarium oxysporum and Sclerotinia sclerotiorum respectively, and after cultivating in the dark at 25°C for 5 days, cut with a scalpel at the edge of the colony respectively. Take 0.5×0.5cm mycelium pieces and place them in new PDA petri dishes (containing 0, 200, 400, 600, 800, 1000 μg / mL glycyrrhizic acid respectively), and incubate in the dark at 25°C for 5 days without adding glycyrrhizic acid. Petri dishes were used as controls.
[0031] see Figure 1-2 According to the statistics of the growth rate of pathogenic bacteria, it was found that 1000μg / mL glycyrrhizic acid had obvious inhibitory effects on the growth and cell morphology of the tested strains of litchi downy mildew and soybean root rot.
Embodiment 2
[0033] On the ultra-clean workbench, firstly inoculate PDA medium with Pythophthora litchii, Phytophthora sojae, Fusarium oxysporum and Sclerotinia isolated from Fujian respectively, and after cultivating in the dark at 25°C for 5 days, surgically Cut out 0.5×0.5cm mycelium pieces with a blade and put them in a new PDA petri dish (containing 600 μg / mL glycyrrhizic acid), and incubate in the dark at 25°C for 5 days, as the test group (GA), and without adding glycyrrhizic acid as the control group (CK).
[0034] combined with image 3 , Microscopic observation found that compared with the control group, the top growth of the mycelia of the four pathogenic bacteria in the test group was inhibited, the separation distance was shortened, and the branching phenomenon appeared.
[0035] The cell membranes of four pathogenic bacteria were stained with SYTOX green dead cell nucleic acid dye, and the effect of glycyrrhizic acid on the formation of the cell membranes of the four pathoge...
Embodiment 3
[0038] Choose snow peas and lychee leaves with the same size and same leaf age, cut them off together with the petioles, keep the petioles moist with cotton balls, and spread the leaves face up in a wet filter paper petri dish with a diameter of 15 cm. Glycyrrhizic acid powder was prepared to a concentration of 600 μg mL -1 The liquid medicine is ready for use. Spray the medicine on the snow pea and litchi leaves first, spray the medicine for 24 hours and wait for the medicine solution to dry in the shade, then inoculate, and use a puncher with a diameter of 5mm to inoculate the edge 1 / 3 of the colonies of Fusarium oxysporum and Lychee peronosporum that have been cultured for 2-3 days Bacteria cakes were taken and each leaf was inoculated with one cake. Distilled water was used as the blank control group. Each treatment was repeated 3 times, and 6 leaves were selected for each repetition. At 26°C, light 12h / darkness 12h were alternately cultivated for 2-3d, and the size of le...
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