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Method for simultaneously determining multiple plasticizers and multiple pesticide residues in fruits

A technology for pesticide residues and determination methods, which is applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problems of poisoning and determination of fruits at the same time, and achieve the effect of low cost, simple method, and no chemical reagent residue

Pending Publication Date: 2021-04-06
河北省农林科学院生物技术与食品科学研究所
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Fruits are not only rich in vitamin nutrition, but also can promote digestion. In today's era of extensive use of pesticides, various chemical preservatives and preservatives, and various growth hormones in fruits, every family was completely in a state of no resistance before this. The state can only be passively accepted, and is in a process of being poisoned by pesticides and other chemicals every day. For example, various cancers and cardiovascular diseases are related to long-term intake of excessive pesticides and various chemicals. Method for Simultaneous Determination of Multiple Plasticizers and Multiple Pesticide Residues in Fruit

Method used

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  • Method for simultaneously determining multiple plasticizers and multiple pesticide residues in fruits
  • Method for simultaneously determining multiple plasticizers and multiple pesticide residues in fruits
  • Method for simultaneously determining multiple plasticizers and multiple pesticide residues in fruits

Examples

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Effect test

Embodiment 1

[0023] Example 1, a method for simultaneously determining multiple plasticizers and multiple pesticide residues in fruits, the determination method includes the following steps: S1: Extraction: Weigh 10 g of fruit samples in a 50 mL centrifuge tube, add 10 mL of acetonitrile, 4 g Magnesium sulfate, 1g sodium chloride, 1g sodium citrate, 0.5g disodium hydrogen citrate and 1 ceramic homogenate, cover the cap of the centrifuge tube, shake vigorously for 1min, then centrifuge at 4200r / min for 5min; S2: Solution preparation: Transfer 1mL of 100μg / mL epoxyheptachloride B standard solution to a 10mL volumetric flask, and dilute the internal standard stock solution with ethyl acetate to 5mg / L before use to obtain the internal standard solution; S3: Purification: Add 6 mL of supernatant to a 15 mL glass centrifuge tube containing 900 mg of magnesium sulfate and 150 mg of PSA, vortex and mix for 1 min, centrifuge at 4200 r / min for 5 min, accurately pipette 1 mL of supernatant into a 10 m...

Embodiment 2

[0024] Example 2, a method for simultaneously determining multiple plasticizers and multiple pesticide residues in fruits, the determination method includes the following steps: S1: Extraction: Weigh 10 g of fruit samples in a 50 mL centrifuge tube, add 10 mL of acetonitrile, 4 g Magnesium sulfate, 1g sodium chloride, 1g sodium citrate, 0.5g disodium hydrogen citrate and 1 ceramic homogenate, cover the centrifuge tube cover, shake vigorously for 1min, centrifuge at 4200r / min for 5min; the fruit sample in S1 passes Weigh with an electronic balance, and the weight is accurate to 0.01g; S1 uses a centrifuge and a shaker to vibrate and centrifuge the solute in the centrifuge tube; S2: Solution preparation: transfer 1mL100μg / mL epoxyheptachloro B standard solution to In a 10mL volumetric flask, dilute ethyl acetate as the internal standard stock solution. Before use, dilute the internal standard stock solution with ethyl acetate to 5mg / L to obtain the internal standard solution; S3:...

Embodiment 3

[0025] Example 3, a method for simultaneously determining multiple plasticizers and multiple pesticide residues in fruits, the determination method includes the following steps: S1: Extraction: Weigh 10 g of fruit samples in a 50 mL centrifuge tube, add 10 mL of acetonitrile, 4 g Magnesium sulfate, 1g sodium chloride, 1g sodium citrate, 0.5g disodium hydrogen citrate and 1 ceramic homogenate, cover the centrifuge tube cover, shake vigorously for 1min, centrifuge at 4200r / min for 5min; the fruit sample in S1 passes Weigh with an electronic balance, and the weight is accurate to 0.01g; S1 uses a centrifuge and a shaker to vibrate and centrifuge the solute in the centrifuge tube; S2: Solution preparation: transfer 1mL100μg / mL epoxyheptachloro B standard solution to In a 10mL volumetric flask, dilute ethyl acetate as the internal standard stock solution. Before use, dilute the internal standard stock solution with ethyl acetate to 5mg / L to obtain the internal standard solution; S3:...

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Abstract

The invention discloses a method for simultaneously determining multiple plasticizers and multiple pesticide residues in fruits in the field of fruit detection, which comprises the following steps: S1: extraction: weighing 10g of fruit sample in a 50mL centrifuge tube, adding 10mL of acetonitrile, 4g of magnesium sulfate, 1g of sodium chloride, 1g of sodium citrate, 0.5g of disodium hydrogen citrate and one ceramic homoproton, covering the centrifuge tube, and after violently oscillating for 1 minute, centrifuging for 5 minutes at the speed of 4200 r / min; and S2, preparing a solution, namely transferring 1mL of 100 [mu]g / mL epoxy heptachloroB standard solution into a 10mL volumetric flask, fixing the volume to be an internal standard stock solution by using ethyl acetate, and diluting the internal standard stock solution to 5mg / L by using the ethyl acetate before use to obtain an internal standard solution. According to the invention, a sample is subjected to acetonitrile homogenate extraction, salting-out centrifugation, Quickers purification and nitrogen blowing, then an internal standard substance (epoxy heptachloroB) is added and redissolved with ethyl acetate, gas chromatography tandem mass spectrometry is used for determination, an internal standard method is used for quantification, and the method is simple, low in cost, safe, environmentally friendly and free of chemical reagent residues.

Description

technical field [0001] The invention relates to the field of fruit detection, in particular to a method for simultaneously measuring multiple plasticizers and multiple pesticide residues in fruits. Background technique [0002] Fruit refers to edible plant fruit that is juicy and mainly tastes sweet and sour. Fruits are not only rich in vitamin nutrition, but also can promote digestion. In today's era of extensive use of pesticides, various chemical preservatives and preservatives, and various growth hormones in fruits, every family was completely in a state of no resistance before this. The state can only be passively accepted, and is in a process of being poisoned by pesticides and other chemicals every day. For example, various cancers and cardiovascular diseases are related to long-term intake of excessive pesticides and various chemicals. A method for the simultaneous determination of multiple plasticizers and multiple pesticide residues in fruit. Therefore, those ski...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/88
CPCG01N30/88G01N2030/8809
Inventor 钱训郑振山陈勇达张嘉坤李丽梅张少军
Owner 河北省农林科学院生物技术与食品科学研究所
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