Supercharge Your Innovation With Domain-Expert AI Agents!

Egg yolk antibody for inhibiting new coronavirus SARS-CoV-2 and preparation method and application of egg yolk antibody

A yolk antibody, sars-cov-2 technology, applied in the field of biomedicine, can solve the problems of potential danger, limited plasma source, uncomfortable promotion, etc., and achieve the effects of no toxic side effects, convenient use, and prevention of severe acute respiratory syndrome.

Inactive Publication Date: 2021-04-09
南京蛋球球生物医学技术合伙企业(有限合伙)
View PDF3 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The use of SARS-CoV-2 neutralizing antibodies in this response can effectively treat severe acute respiratory syndrome caused by SARS-CoV-2, but the plasma sources of cured patients are limited, and the processing procedures are complicated, which is potentially dangerous and not suitable for widespread promotion
[0006] In the prior art implementation, there is no report of anti-SARS-CoV-2 egg yolk antibody

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] 1. Preparation of antigen by gene recombination method:

[0038] The epitope of S protein is:

[0039] S1 IAWNSNNLDSKVGGNYNYLY

[0040] S2 LKPFERDISTEIYQ

[0041] S3 AVRDPQTLEILDIT

[0042] S4 VEQDKNTQEVFAQV

[0043] S5 DPSKPSKRSFIED

[0044] S6 NHTSPDVDLGDISGIN;

[0045] (11) Competent preparation of Escherichia coli: inoculate the prokaryotic expression bacteria BL21, Rosetta or OrigamiB by streaking onto LB culture plates, and put them in an incubator at 35-40°C overnight. On the second day, pick a single colony and place it in LB medium, culture overnight on a shaker at 35-40°C, 200-300rpm; on the third day, inoculate the overnight cultured bacterial solution into fresh LB medium at a ratio of 1:100, Cultivate at 35-40°C, 3000-5000 rpm for 1-2 hours until OD600 reaches 0.6. Transfer the bacterial culture solution into a centrifuge tube, place it on ice for 5-15 minutes, centrifuge at 3000-5000rpm at 4°C for 10 minutes; discard the supernatant, and use pre-coo...

Embodiment 2

[0058] Example 2: The specific process of this example is basically the same as that in Example 1, the difference being that the specific process of extracting antibodies from chicken eggs is as follows: PEG precipitation method is used to extract egg yolk antibodies: egg yolks are separated from collected immunized chicken eggs, Add 4% PEG 3 times the volume of egg yolk to dilute, stir well, centrifuge at 12000rpm for 10 minutes at 4°C, take the supernatant, and then add 1 / 6 of the volume of the supernatant dropwise to the supernatant After 40% PEG was fully stirred and mixed, centrifuged under the same conditions, the precipitate was collected and freeze-dried to obtain egg yolk antibody.

[0059] The egg yolk antibody in this embodiment can be used to prepare a spray, and the specific formula is: 1 g of egg yolk antibody for inhibiting the new coronavirus SARS-CoV-2, disodium hydrogen phosphate (Na 2 HPO 4 2H 2 O) 4.45g, sodium dihydrogen phosphate (NaH 2 PO 4 ·H 2 O) ...

Embodiment 3

[0060] Embodiment 3: The specific process of this embodiment is basically the same as that of Example 1, the difference being that the specific process of extracting antibodies from chicken eggs is: extracting yolk antibodies by ammonium sulfate precipitation: separating the volume from the collected immunized chicken eggs is V obtained egg yolk, add normal saline with a volume of V, slowly add 50% saturated ammonium sulfate with a volume of 2V dropwise, stir slowly at 4°C for 3 hours, centrifuge at 12000rpm for 10 minutes, discard the supernatant, dissolve the precipitate with normal saline, add 40% saturated ammonium sulfate with a volume of 2 / 3V, stirred slowly at 4°C for 3 hours, centrifuged at 12000rpm for 10 minutes, discarded the supernatant, dissolved the precipitate with normal saline, added 33% saturated ammonium sulfate with a volume of 1 / 2V, Stir slowly at 4°C for 3 hours, take the precipitate and freeze-dry to obtain egg yolk antibody.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
diameteraaaaaaaaaa
Login to View More

Abstract

The invention discloses a preparation method of an egg yolk antibody for inhibiting a new coronavirus SARS-CoV-2. The preparation method comprises the following steps: preparing an S protein antigen of the SARS-CoV-2 coronavirus by adopting a gene recombination method; emulsifying the antigen; performing immunization: selecting healthy egg laying hens for isolated feeding, and performing injection immunization; and extracting the antibody from the chicken eggs. An antigen epitope expression plasmid containing SARS-CoV-2 coronavirus S proteins is constructed by using a genetic engineering method and expressed in escherichia coli, then an expression product is separated and purified to serve as an antigen, the antigen is mixed and emulsified with an adjuvant, healthy egg laying hens are immunized, eggs are collected, an egg yolk antibody in egg yolk is extracted, the antibody is natural immunoglobulin, and the SARS-CoV-2 coronavirus can be effectively inhibited and eliminated.

Description

technical field [0001] The invention belongs to the technical field of biomedicine, and in particular relates to an egg yolk antibody for inhibiting the new coronavirus SARS-CoV-2 and its preparation method and application. Background technique [0002] The clinical features of the new coronavirus include fever, fatigue, and dry cough. A small number of patients are accompanied by symptoms such as nasal congestion, runny nose, and diarrhea. The incubation period is generally between 1 and 14 days. During the incubation period, there is a risk of infection. The mode of transmission It mainly includes direct droplet transmission and contact transmission. [0003] The SARS-CoV-2 virus belongs to the βcoronavirus, and the virus particles are mostly round or oval, with an envelope, and there are coronal-arranged fibrils around the periphery, and the diameter of the virus is between 60-140nm. Its genetic characteristics are significantly different from those of SARSr-Cov and MERS...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C07K16/10C07K16/02A61K39/42A61K9/08A61K9/12A61K47/12A61P31/14A61P11/00
CPCA61K9/08A61K9/12A61K47/12A61K2039/505A61P11/00A61P31/14C07K16/02C07K16/10C07K2317/76
Inventor 唐小军杨严俊杨昌顺张秀芳张小莺
Owner 南京蛋球球生物医学技术合伙企业(有限合伙)
Features
  • R&D
  • Intellectual Property
  • Life Sciences
  • Materials
  • Tech Scout
Why Patsnap Eureka
  • Unparalleled Data Quality
  • Higher Quality Content
  • 60% Fewer Hallucinations
Social media
Patsnap Eureka Blog
Learn More

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2025 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com