Method for detecting effective components of stomach-invigorating syrup for children
A detection method and pediatric technology, applied in the field of medicine, can solve problems such as the inability to comprehensively monitor the quality of medicines
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Embodiment 1
[0076] Example 1 Identification item
[0077] Instruments and reagents
[0078] DigiStore 3 digital imaging system (Switzerland CAMAG); double-slot expansion cylinder (Shanghai Xinyi Instrument Factory);
[0079] Silica gel G prefabricated plate (Qingdao Ocean Chemical Factory), silica gel G prefabricated plate (Zhejiang Province Luqiao Sijia Biochemical Plastic Factory); TLC Silica 60 (Merck ordinary silica gel G plate); self-made plate (with 0.3% carboxymethyl cellulose Sodium-based silica gel G plate (thickness: 500 μm); self-made plate (silica gel G plate containing 1% sodium hydroxide).
[0080] Chemical reagents: cyclohexane, ethyl acetate, methanol, glacial acetic acid, 95% ethanol, sodium hydroxide, all of which are of analytical grade.
[0081] Reference substances and reference medicinal materials: lotus leaf reference medicinal material (batch number: 121008-200505); tangerine peel reference medicinal material (lot number: 120904-200512); hawthorn reference medici...
Embodiment 2
[0091] Embodiment two [content determination] the content determination of paeoniflorin and hesperidin
[0092] 1 Instruments and reagents
[0093] High performance liquid chromatography: Agilent 1200.
[0094] Acetonitrile is chromatographically pure, water is purified water, and other reagents are analytically pure.
[0095] Paeoniflorin reference substance (batch number 110736-201135, content 96.5%; 110736-201136 content 96.0%; 110736-201842 content 97.4%;), hesperidin reference substance (batch number 110721-201818, content In terms of 96.2%), all of the above are provided by China National Institutes for Food and Drug Control. Pediatric Jianwei syrup samples and negative samples were provided by Guangzhou Wanglaoji Pharmaceutical Co., Ltd.
[0096] 2 Chromatographic conditions
[0097] Chromatographic column: Welch ultimate XB-C18 (4.6×250mm, 5μm)
[0098] Kromasil 100-5 C18 (4.6×250mm, 5μm)
[0099] Capcell Pak MG S5 C18 (4.6×250mm, 5μm)
[0100] Mobile phase: ace...
Embodiment 3
[0110] Example 3 Methodology Verification
[0111] 5.1 Preparation of each solution
[0112] Preparation of reference substance solution: Accurately weigh paeoniflorin and hesperidin reference substances and put them in measuring bottles respectively, add an appropriate amount of methanol to dissolve and dilute to the scale, and prepare paeoniflorin reference substance stock solution (about 0.25mg / ml) and orange Cortexin reference substance stock solution (about 0.4mg / ml).
[0113] Accurately draw the above two reference substance stock solutions respectively, add methanol to dilute and constant volume, and prepare paeoniflorin reference substance solution (about 50 μg / ml) and hesperidin reference substance solution (about 30 μg / ml).
[0114] Preparation of the test solution: Accurately draw 5ml of this product, place it in a 10ml measuring bottle, dilute to the mark with methanol, shake well, centrifuge, take the supernatant, filter through a 0.45um microporous membrane, and...
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