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Streptomyces lilacinus and application thereof in plant disease control

A technology for plant disease control and Streptomyces syringae, applied in the fields of application, plant growth regulators, and microbial-based methods, can solve the problems of obtaining new actinomycetes that have not yet been discovered

Active Publication Date: 2021-04-13
ZHEJIANG NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0018] In the research on the isolation and screening of antagonistic microorganisms for the control of soybean bacterial spot disease, no new reports of actinomycetes have been found for the control of the disease

Method used

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  • Streptomyces lilacinus and application thereof in plant disease control
  • Streptomyces lilacinus and application thereof in plant disease control
  • Streptomyces lilacinus and application thereof in plant disease control

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] Embodiment 1, the screening and identification of Streptomyces lilacinus (Streptomyces lilacinus) S1-3 bacterial strain

[0038] 1 strain

[0039] (1) Actinomycetes strains: isolate, purify, screen and identify actinomycetes strains from soils in different habitats (for example: paddy field soil, soybean field soil, lawn soil, camellia field soil, tomato field soil, etc.), and preserve actinomycete strains.

[0040] (2) Xanthomonas axonopodis pv. glycines (hereinafter abbreviated as Xag) is a soybean pathogenic bacterium, which is used as an indicator bacterium for the screening of actinomycete antagonism.

[0041] 2 media

[0042] (1) Gaoshi No. 1 solid medium: soluble starch 20g, KNO 3 1g, K 2 HPO 4 0.5g, MgSO 4 ·7H 2 O0.5g, NaCl 0.5g, FeSO 4 ·7H 2 O 0.01g, agar 20g, water 1000mL, pH 7.1. For the isolation, purification and identification of actinomycete strains.

[0043] (2) Gao Shi No. 1 liquid medium: the formula is the same as (1), but without adding a...

Embodiment 2

[0080] Embodiment 2, Streptomyces lilacinus (Streptomyces lilacinus) S1-3 bacterial strain antimicrobial spectrum determination

[0081] 1 strain

[0082] (1) Streptomyces lilacinus S1-3 strain.

[0083] (2) Six representative plant pathogenic bacteria: Xanthomonas oryzaepv.oryzicola, Pseudomonas suringae pv.glycinea, Xanthomonas campestris pv.campestris , tomato bacterial leaf spot (Pseudomonassyringae pv.tomato), tobacco bacterial wilt (Ralstortia solonacearum), rice bacterial blight (Xanthomonas oryzae pv.oryzae) 6 representative plant pathogenic bacteria (see Table 3), for Determination of antibacterial spectrum of fermentation filtrate of Sl-3 strain.

[0084] 2 media

[0085] (1) Beef extract peptone liquid medium (beef extract peptone culture medium): 3g beef extract, 10g peptone, 5g NaCl, add water to 1000mL, adjust pH to 7.4;

[0086] Beef extract peptone agar solid medium: Add 18g of agar powder on the basis of beef extract peptone liquid medium.

[0087] For th...

Embodiment 3

[0101] Example 3 Streptomyces S1-3 strain fermented filtrate preventive effect research on soybean bacterial spot disease

[0102] 1 Germs and Soybean Varieties

[0103] (1) Actinomycetes used for control effect: Streptomyces lilacinus Sl-3 strain.

[0104] (2) Pathogen: Soybean Bacterial Spot Disease (Xanthomonas axonopodis pv.glycines, Xag)

[0105] (3) Soybean variety: Zhoudou No. 36 variety susceptible to soybean bacterial spot disease was selected.

[0106] 2 Media and pesticides

[0107] (1) Gao Shi No. 1 solid and liquid medium, used for the cultivation, fermentation and preparation of fermentation filtrate of S1-3 bacterial strain.

[0108] (2) NA medium: beef extract 3g, peptone 5g, glucose 2.5g, agar 15-20g, water 1000mL, pH 7.2 (liquid medium without agar). For the cultivation of Xag bacteria.

[0109] (3) Bordeaux mixture pesticide (1:1:200): copper sulfate 5g, quicklime 5g, water 1000mL.

[0110] 3 Experimental methods

[0111] 3.1 Cultivation of soybean se...

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Abstract

The invention belongs to the field of microbial biological control, and discloses streptomyces lilacinus Sl-3, the preservation number of which is CCTCC NO: M 2020423. The invention also discloses application of the streptomyces lilacinus Sl-3. The streptomyces lilacinus Sl-3 is used for preventing and treating plant diseases, and especially used for biological prevention and treatment of Xanthomonas campestris pv.

Description

technical field [0001] The invention belongs to the field of microbial biological control, and relates to antagonizing soybean bacterial spot pathogen Streptomyces lilacinus (Streptomyces lilacinus) S1-3 strain and its application in plant disease control. Background technique [0002] Bacterial diseases of soybean run through the entire growth period of soybean, causing damage to soybean seedlings, leaves, and pods, resulting in a decline in soybean yield and quality. The disease is more serious in the warm and humid environment with high temperature and rain, and it is prevalent and occurs to varying degrees in the main soybean producing areas in the north and south of China. [0003] The pathogen of soybean bacterial spot disease is Xanthomonas axonopodis pv. glycines, Xag. The typical symptom at the time of its onset is that the lesions appear as light brown dots at the beginning, and then transform into small dark brown polygonal spots with a size of 1-2 mm, with yello...

Claims

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Application Information

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IPC IPC(8): C12N1/20A01N63/28A01P1/00C12R1/465
CPCA01N63/28
Inventor 蒋冬花倪峰郭鑫王炫栋杨孙玉悦胡凌鸣徐春毅
Owner ZHEJIANG NORMAL UNIVERSITY
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