Streptomyces lilacinus and application thereof in plant disease control
A technology for plant disease control and Streptomyces syringae, applied in the fields of application, plant growth regulators, and microbial-based methods, can solve the problems of obtaining new actinomycetes that have not yet been discovered
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Embodiment 1
[0037] Embodiment 1, the screening and identification of Streptomyces lilacinus (Streptomyces lilacinus) S1-3 bacterial strain
[0038] 1 strain
[0039] (1) Actinomycetes strains: isolate, purify, screen and identify actinomycetes strains from soils in different habitats (for example: paddy field soil, soybean field soil, lawn soil, camellia field soil, tomato field soil, etc.), and preserve actinomycete strains.
[0040] (2) Xanthomonas axonopodis pv. glycines (hereinafter abbreviated as Xag) is a soybean pathogenic bacterium, which is used as an indicator bacterium for the screening of actinomycete antagonism.
[0041] 2 media
[0042] (1) Gaoshi No. 1 solid medium: soluble starch 20g, KNO 3 1g, K 2 HPO 4 0.5g, MgSO 4 ·7H 2 O0.5g, NaCl 0.5g, FeSO 4 ·7H 2 O 0.01g, agar 20g, water 1000mL, pH 7.1. For the isolation, purification and identification of actinomycete strains.
[0043] (2) Gao Shi No. 1 liquid medium: the formula is the same as (1), but without adding a...
Embodiment 2
[0080] Embodiment 2, Streptomyces lilacinus (Streptomyces lilacinus) S1-3 bacterial strain antimicrobial spectrum determination
[0081] 1 strain
[0082] (1) Streptomyces lilacinus S1-3 strain.
[0083] (2) Six representative plant pathogenic bacteria: Xanthomonas oryzaepv.oryzicola, Pseudomonas suringae pv.glycinea, Xanthomonas campestris pv.campestris , tomato bacterial leaf spot (Pseudomonassyringae pv.tomato), tobacco bacterial wilt (Ralstortia solonacearum), rice bacterial blight (Xanthomonas oryzae pv.oryzae) 6 representative plant pathogenic bacteria (see Table 3), for Determination of antibacterial spectrum of fermentation filtrate of Sl-3 strain.
[0084] 2 media
[0085] (1) Beef extract peptone liquid medium (beef extract peptone culture medium): 3g beef extract, 10g peptone, 5g NaCl, add water to 1000mL, adjust pH to 7.4;
[0086] Beef extract peptone agar solid medium: Add 18g of agar powder on the basis of beef extract peptone liquid medium.
[0087] For th...
Embodiment 3
[0101] Example 3 Streptomyces S1-3 strain fermented filtrate preventive effect research on soybean bacterial spot disease
[0102] 1 Germs and Soybean Varieties
[0103] (1) Actinomycetes used for control effect: Streptomyces lilacinus Sl-3 strain.
[0104] (2) Pathogen: Soybean Bacterial Spot Disease (Xanthomonas axonopodis pv.glycines, Xag)
[0105] (3) Soybean variety: Zhoudou No. 36 variety susceptible to soybean bacterial spot disease was selected.
[0106] 2 Media and pesticides
[0107] (1) Gao Shi No. 1 solid and liquid medium, used for the cultivation, fermentation and preparation of fermentation filtrate of S1-3 bacterial strain.
[0108] (2) NA medium: beef extract 3g, peptone 5g, glucose 2.5g, agar 15-20g, water 1000mL, pH 7.2 (liquid medium without agar). For the cultivation of Xag bacteria.
[0109] (3) Bordeaux mixture pesticide (1:1:200): copper sulfate 5g, quicklime 5g, water 1000mL.
[0110] 3 Experimental methods
[0111] 3.1 Cultivation of soybean se...
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